To accurately identify soybean pests and diseases, in this paper, a kind of deep convolution network model was used to determine whether or not a soybean crop possessed pests and diseases. The proposed deep convolutio...To accurately identify soybean pests and diseases, in this paper, a kind of deep convolution network model was used to determine whether or not a soybean crop possessed pests and diseases. The proposed deep convolution network could learn the highdimensional feature representation of images by using their depth. An inception module was used to construct a neural network. In the inception module, multiscale convolution kernels were used to extract the distributed characteristics of soybean pests and diseases at different scales and to perform cascade fusion. The model then trained the SoftMax classifier in a uniformed framework. This realized the model of soybean pests and diseases so as to verify the effectiveness of this method. In this study, 800 images of soybean leaf images were taken as the experimental objects. Of these 800 images, 400 were selected for network training, and the remaining 400 images were used for the network test. Furthermore, the classical convolutional neural network was optimized. The accuracies before and after optimization were 96.25% and 95.81%, respectively, in terms of extracting image features. This type of research might be applied to achieve a degree of automation in agricultural field management.展开更多
目的探讨来源于冷冻整鸡样本的单株猪红斑丹毒丝菌致病性、耐药性及生物学特征,为该菌防治提供科学依据。方法通过基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry,...目的探讨来源于冷冻整鸡样本的单株猪红斑丹毒丝菌致病性、耐药性及生物学特征,为该菌防治提供科学依据。方法通过基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)和全基因组测序技术进行菌株鉴定和分子生物学研究,采用纸片扩散法(K-B法)检测其耐药表型,通过体外生物被膜形成实验检测其生物被膜形成能力,通过ParSNP构建亲缘关系图。结果MALDI-TOF MS可以准确鉴定猪红斑丹毒丝菌,该菌染色体大小为1791362 bp,鸟嘌呤(G)+胞嘧啶(C)含量为36.5%,含有1730个蛋白质编码序列,53个tRNA和2个rRNA,基因组分析暂未发现质粒;携带四环素类耐药基因tet(M),抗菌药物敏感性实验结果显示仅对四环素耐药,耐药基因和耐药表型一致;携带7个与黏附和荚膜形成相关的毒力基因;体外生物被膜形成能力适中(2+);与韩国分离株KC-Sb-R1亲缘关系较相近。结论本研究阐明了冷冻整鸡样本中猪红斑丹毒丝菌分离株的生物学特性,为该菌科学有效防控提供理论依据。展开更多
目的分析TFRs占Tregs的比例与HIV感染疾病进展的关系。方法以2016—2020年于汉中三二〇一医院门诊就诊及随访的94例HIV感染者(包括未治疗者,抗病毒治疗免疫重建成功和免疫重建失败者)及性别、年龄等相匹配的健康对照为研究对象。将CD3^(...目的分析TFRs占Tregs的比例与HIV感染疾病进展的关系。方法以2016—2020年于汉中三二〇一医院门诊就诊及随访的94例HIV感染者(包括未治疗者,抗病毒治疗免疫重建成功和免疫重建失败者)及性别、年龄等相匹配的健康对照为研究对象。将CD3^(+)CD4^(+)Foxp3^(+)T细胞定义为调节性T细胞(regulatory T cells,Tregs),将CD3^(+)CD4^(+)CXCR5^(+)Foxp3^(+)T细胞定义为滤泡调节性T细胞(follicular regulatory T cells,TFRs),分析TFRs占Tregs的比例与HIV RNA及CD4^(+)T细胞计数之间的关系,并对比分析其在免疫重建成功与免疫重建失败HIV感染者之间的差异。结果TFRs/Tregs比值与HIV RNA呈负相关(r=-0.276,P<0.05),与CD4^(+)T细胞计数呈正相关(r=0.579,P<0.05)。结论HIV感染者TFRs占Tregs的比例越高,机体的免疫状态有可能越好。展开更多
Objective:To study the mechanism of Shengmai Injection(SMI)on anti-sepsis and protective activities of intestinal mucosal barrier.Methods:The contents of 11 active components of SMI including ginsenoside Rb1,Rb2,Rb3,R...Objective:To study the mechanism of Shengmai Injection(SMI)on anti-sepsis and protective activities of intestinal mucosal barrier.Methods:The contents of 11 active components of SMI including ginsenoside Rb1,Rb2,Rb3,Rd,Re,Rf,Rg1,Rg2,ophioposide D,schisandrol A and schisantherin A were determined using ultra-performance liquid chromatography.Fifty mice were randomly divided into the blank,the model,the low-,medium-and high-dose SMI groups(0.375,0.75,1.5 mL/kg,respectively)by random number table,10 mice in each group.On SMI group,SMI was administrated to mice daily via tail vein injection for 3 consecutive days,while the mice in the blank and model groups were given 0.1 mL of normal saline.One hour after the last SMI administration,except the blank group,the mice in other groups were intraperitoneally injected with lipopolysaccharide(LPS)saline solution(2 mL/kg)at a dosage of 5 mL/kg for development of endotoxemia mice model.The mice in the blank group were given the same volume of normal saline.Inflammatory factors including interferon-γ(INF-γ),tumor necrosis factor-α(TNF-α),interleukin(IL)-2 and IL-10 were measured by flow cytometry.Myosin light-chain kinase(MLCK),nuclear factorκB(NF-κB)levels,and change of Occludin proteins in jejunum samples were analyzed by Western blot.Results:The decreasing trends of INF-γ,TNF-α and IL-2 were found in serum of SMI treatment groups.In SMI-treated mice,the content of Occludin increased and MLCK protein decreased compared with the model group(P<0.05 or P<0.01).The content of cellular and nuclear NF-κB did not change significantly(P>0.05).Conclusion:SMI may exert its anti-sepsis activity mainly through NF-κB-pro-inflammatory factor-MLCK-TJ cascade.展开更多
基金Supported by 2017 Harbin Application Technology Research and Development Funds Innovation Talent Project(2017RAQXJ079)
文摘To accurately identify soybean pests and diseases, in this paper, a kind of deep convolution network model was used to determine whether or not a soybean crop possessed pests and diseases. The proposed deep convolution network could learn the highdimensional feature representation of images by using their depth. An inception module was used to construct a neural network. In the inception module, multiscale convolution kernels were used to extract the distributed characteristics of soybean pests and diseases at different scales and to perform cascade fusion. The model then trained the SoftMax classifier in a uniformed framework. This realized the model of soybean pests and diseases so as to verify the effectiveness of this method. In this study, 800 images of soybean leaf images were taken as the experimental objects. Of these 800 images, 400 were selected for network training, and the remaining 400 images were used for the network test. Furthermore, the classical convolutional neural network was optimized. The accuracies before and after optimization were 96.25% and 95.81%, respectively, in terms of extracting image features. This type of research might be applied to achieve a degree of automation in agricultural field management.
文摘目的探讨来源于冷冻整鸡样本的单株猪红斑丹毒丝菌致病性、耐药性及生物学特征,为该菌防治提供科学依据。方法通过基质辅助激光解吸电离飞行时间质谱法(matrix assisted laser desorption ionization time of flight mass spectrometry,MALDI-TOF MS)和全基因组测序技术进行菌株鉴定和分子生物学研究,采用纸片扩散法(K-B法)检测其耐药表型,通过体外生物被膜形成实验检测其生物被膜形成能力,通过ParSNP构建亲缘关系图。结果MALDI-TOF MS可以准确鉴定猪红斑丹毒丝菌,该菌染色体大小为1791362 bp,鸟嘌呤(G)+胞嘧啶(C)含量为36.5%,含有1730个蛋白质编码序列,53个tRNA和2个rRNA,基因组分析暂未发现质粒;携带四环素类耐药基因tet(M),抗菌药物敏感性实验结果显示仅对四环素耐药,耐药基因和耐药表型一致;携带7个与黏附和荚膜形成相关的毒力基因;体外生物被膜形成能力适中(2+);与韩国分离株KC-Sb-R1亲缘关系较相近。结论本研究阐明了冷冻整鸡样本中猪红斑丹毒丝菌分离株的生物学特性,为该菌科学有效防控提供理论依据。
文摘目的分析TFRs占Tregs的比例与HIV感染疾病进展的关系。方法以2016—2020年于汉中三二〇一医院门诊就诊及随访的94例HIV感染者(包括未治疗者,抗病毒治疗免疫重建成功和免疫重建失败者)及性别、年龄等相匹配的健康对照为研究对象。将CD3^(+)CD4^(+)Foxp3^(+)T细胞定义为调节性T细胞(regulatory T cells,Tregs),将CD3^(+)CD4^(+)CXCR5^(+)Foxp3^(+)T细胞定义为滤泡调节性T细胞(follicular regulatory T cells,TFRs),分析TFRs占Tregs的比例与HIV RNA及CD4^(+)T细胞计数之间的关系,并对比分析其在免疫重建成功与免疫重建失败HIV感染者之间的差异。结果TFRs/Tregs比值与HIV RNA呈负相关(r=-0.276,P<0.05),与CD4^(+)T细胞计数呈正相关(r=0.579,P<0.05)。结论HIV感染者TFRs占Tregs的比例越高,机体的免疫状态有可能越好。
基金Supported by National Natural Science Foundation of China(No.81673667)Chinese Academy of Medical Science(Peking Union Medical College)Innovation Fund for Medical Science(CIFMS,No.2016-I2M-3-015 and No.2017-I2M-B&R-09)。
文摘Objective:To study the mechanism of Shengmai Injection(SMI)on anti-sepsis and protective activities of intestinal mucosal barrier.Methods:The contents of 11 active components of SMI including ginsenoside Rb1,Rb2,Rb3,Rd,Re,Rf,Rg1,Rg2,ophioposide D,schisandrol A and schisantherin A were determined using ultra-performance liquid chromatography.Fifty mice were randomly divided into the blank,the model,the low-,medium-and high-dose SMI groups(0.375,0.75,1.5 mL/kg,respectively)by random number table,10 mice in each group.On SMI group,SMI was administrated to mice daily via tail vein injection for 3 consecutive days,while the mice in the blank and model groups were given 0.1 mL of normal saline.One hour after the last SMI administration,except the blank group,the mice in other groups were intraperitoneally injected with lipopolysaccharide(LPS)saline solution(2 mL/kg)at a dosage of 5 mL/kg for development of endotoxemia mice model.The mice in the blank group were given the same volume of normal saline.Inflammatory factors including interferon-γ(INF-γ),tumor necrosis factor-α(TNF-α),interleukin(IL)-2 and IL-10 were measured by flow cytometry.Myosin light-chain kinase(MLCK),nuclear factorκB(NF-κB)levels,and change of Occludin proteins in jejunum samples were analyzed by Western blot.Results:The decreasing trends of INF-γ,TNF-α and IL-2 were found in serum of SMI treatment groups.In SMI-treated mice,the content of Occludin increased and MLCK protein decreased compared with the model group(P<0.05 or P<0.01).The content of cellular and nuclear NF-κB did not change significantly(P>0.05).Conclusion:SMI may exert its anti-sepsis activity mainly through NF-κB-pro-inflammatory factor-MLCK-TJ cascade.
