Evaluation of a Wireless Solar Powered Personal Weather Station

We are evaluating dryland cotton production in Martin County, Texas, measuring cotton lint yield per unit of rainfall. Our goal is to collect rainfall data per 250 - 400 ha. Upon selection of a rainfall gauge, we realized that the cost of using, for example, a tipping bucket-type rain gauge would be too expensive and thus searched for an alternative method. We selected an all-in-one commercially available weather station;hereafter, referred to as a Personal Weather Station (PWS) that is both wireless and solar powered. Our objective was to evaluate average measurements of rainfall obtained with the PWS and to compare these to measurements obtained with an automatic weather station (AWS). For this purpose, we installed four PWS deployed within 20 m of the Plant Stress and Water Conservation Meteorological Tower that was used as our AWS, located at USDA-ARS Cropping Systems Research Laboratory, Lubbock, TX. In addition, we measured and compared hourly average values of short-wave irradiance (Rg), air temperature (Tair) and relative humidity (RH), and wind speed (WS), and calculated values of dewpoint temperature (Tdew). This comparison was done over a 242-day period (1 October 2022-31 May 2023) and results indicated that there was no statistical difference in measurements of rainfall between the PWS and AWS. Hourly average values of Rg measured with the PWS and AWS agreed on clear days, but PWS measurements were higher on cloudy days. There was no statistical difference between PWS and AWS hourly average measurements of Tair, RH, and calculated Tdew. Hourly average measurements of Rg and WS were more variable. We concluded that the PWS we selected will provide adequate values of rainfall and other weather variables to meet our goal of evaluating dryland cotton lint yield per unit rainfall.

Scaling Leaf Measurements to Estimate Whole Canopy Gas Exchanges of Cotton

Diurnal leaf and canopy gas exchanges of well-watered field grown cotton were measured. Our objective was to scale leaf-level values of transpiration and net assimilation to the whole canopy level using estimates of canopy leaf area. Single leaf gas exchange measurements were made with two portable photosynthesis systems and canopy measurements with four open Canopy Evapo-Transpiration and Assimilation (CETA) chamber systems. Canopy leaf area was measured at the end of the experiment and estimated during gas exchange by fitting values to a growth curve. Leaf level measurements were arithmetically scaled to estimate canopy level gas exchange based on canopy leaf area and then compared to the measured values. Scaled values of single leaf transpiration were very similar to canopy transpiration measurements, although both whole canopy transpiration and assimilation were overestimated around mid-day. We conclude that canopy cotton transpiration of well-watered field grown plants could be estimated within 5% throughout the day by scaling leaf level measurements to the whole canopy using measured canopy leaf area. Estimating canopy assimilation from leaf level measurements remains problematic.

Stable Carbon Isotope Discrimination (δ13C) of Cotton Burrs and Seeds as a Season-Long Integrator of Crop Water Stress

Plant-based irrigation management schemes typically use surrogates such as canopy temperature, alone, or in conjunction with environmental variables, to infer the degree of “crop stress” (biological strain) induced by drought. Few systematic studies of the relationship between “crop stress”, as defined by such surrogates, and physiological estimates of water use efficiency (WUE) exist over both daily and seasonal time scales relevant to agronomic irrigation control. The systematic application of stable carbon isotope discrimination (δ13C) might allow post hoc evaluation of irrigation scheduling schemes and might also be a useful germplasm screening tool if the source(s) of variability can be uncovered and/or controlled. Results from preliminary efforts comparing leaf and cotton seed δ13C to season-long water deficits showed that seeds are more useful indicators of season-long water stress and water use efficiency during crop development.

Irrigation Termination Thermal Time and Amount on Cotton Lint Yield and Fiber Quality

Cotton irrigation in the Texas High Plains (THP) is often dictated by the well capacity and not by the water needs of the crop. The source of irrigation-water is the Ogallala aquifer and in many areas of the THP, the water table has declined to well capacities that deliver 1.3 to >7.6 mm/d. There is plenty of information on cotton responses to irrigation frequency and amount;however, information on when to terminate irrigation and its effect on cotton lint yield and fiber quality is scarce. Our objective was to evaluate over a 4-year period three irrigation termination thermal times corresponding to cumulative daily heat units (∑HU) of 890 °C, 1000 °C and 1110 °C from crop emergence, and three levels of irrigation (2.5, 5.1 and 7.6 mm/d) on cotton lint yield and fiber quality. Irrigation was applied with a sprinkler system on a 3-day frequency in Lubbock, TX. Results showed that on average the 7.6 mm/d level produced the most cotton lint yield regardless of the irrigation termination thermal time. Terminating cotton at 1000- °C ∑HU resulted in water savings of 25 to 50 mm for the 2.5 and 5.1 mm/d levels without significantly affecting lint yield. For the 7.6 mm/d and terminating at 890- °C ∑HU resulted in water savings of 100 to 115 mm. Average fiber length statistically increased with termination thermal time and level. This effect was most significant in years with the least rain and warmer air temperature. Micronaire increased with the termination thermal time in years with >500 mm of rain. Average fiber length uniformity and fiber strength were minimally affected by termination thermal time. As irrigation level increased, the average micronaire decreased, and fiber strength increased for the 5.1 and 7.6 mm/d irrigation. We concluded that in the THP for well capacities that deliver 2.5 - 5.1 mm/d irrigation can be terminated when the ∑HU reaches about 1000 °C from emergence without impacting cotton lint yield.

Effect of Ambient UV-B on Stomatal Density, Conductance and Isotope Discrimination in Four Field Grown Soybean [<i>Glycine max</i>(L.) Merr.] Isolines

An experiment was designed to test whether ambient levels of UV-B radiation affect stomatal development, decrease stomatal density, and lead to increased water-use efficiency (WUE). Soybean [Glycine max (L.) Merr.] isolines with different stomatal distribution and flavonol expression patterns were field grown under shelters that either transmitted or blocked solar UV-B. All isolines exposed to solar UV-B accumulated higher concentrations of UV-screening phenolic pigments but other responses were isoline dependent. Solar UV-B decreased stomatal density and conductance in isolines expressing a unique branched kaempferol triglycoside. Decreased stomatal density was associated with increased season-long WUE and decreased internal CO2 concentration of leaf (estimated by δ13C discrimination). We concluded that photomorphogenic responses to UV-B affected stomatal density and WUE in field grown soybean;but that the magnitude and direction of these response were associated with isogenic pleiotropic differences in stomatal distribution and pigment expression. UV-B radiation had no effect on biomass accumulation or yield in a cultivar expressing only trace levels of kaempferol suggesting that flavonol expression is not prerequisite to UV-B tolerance.

Canopy Light Interception of a Conventional and an Erect Leafed Sorghum

Two sorghum lines, an erect leafed mutant sorghum and the wild type from which the mutant was generated, were field grown in rectilinear arrays at low (10 plants m-2) and high (23 plants m-2) population densities. Canopy light interception, biomass accretion and yield were measured. Photosynthetically active radiation under the canopy at ground level and midway through the canopy were higher in the erect leafed line, as compared to the normal leafed line. Planting density had less effect on mean grain yield and biomass in the erect leaf line than in the wild type. Though not conclusive, when taken together, the results suggested that optimal planting densities are higher for the erect leaf line and that the erect leaf trait could be useful for incorporation into breeding programs.

Planting Cotton in a Crop Residue in a Semiarid Climate: Water Balance and Lint Yield

Cotton (Gossypium hirsutum L.) is planted on more land area than any other crop on the Texas High Plains. Much of this area is considered highly erodible and requires a conservation compliance program to participate in government farm programs. Because this region is semiarid and because irrigation water is increasingly limited, water conservation and efficient use of water are necessary to maximize cotton lint yields. One popular conservation compliance practice used is to plant cotton into a chemically terminated small grain crop, i.e., residue that provides wind protection to the cotton seedlings. Our hypothesis was that in a semiarid region the use of a small grain cover crop under irrigated conditions would use more water than it conserves compared to conventional tilled cotton, thus reducing cotton lint yields. To test the hypothesis separate field studies over two growing seasons and on two soil textures, a loamy fine sand and a clay loam, were conducted. The main treatments were tillage systems (conventional and conservation using terminated wheat residue). The two split plot treatments were water supply based on replacement of calculated grass reference evapotranspiration (ETo). Tillage did not affect the amount of water used by the cotton crop at either location ( 0.05) except for an 80% ETo irrigation treatment at a single location where the bare soil treatment used 10% more water than the residue treatments for both years. The residue treatment decreased (P < 0.05) cotton lint yields at both locations by 12% except for the 50% ETo single irrigation treatment in which the residue treatment yielded 14% more lint than the bare soil treatment. The use of terminated wheat residue had no impact on soil water storage during any part of the year. During a 5-month period associated with wheat growth, the wheat evapotranspiration was 20 to 40 mm more water (P < 0.05) than that lost through soil water evaporation from the conventional treatments. The use of terminated wheat residue did not benefit the water balance of the cotton crop, and was associated with decreased cotton lint yields. The results were consistent with our working hypothesis, and disproved the idea that planting cotton into wheat stubble cover increases water use efficiency.

Water Balance of Two Major Soil Types of the Texas High Plains: Implications for Dryland Crop Production

Crop production in the Texas High Plains is shifting from irrigated to dryland due to the increase of the depth to the water table from the Ogallala aquifer in regions where the saturated thickness of 9 m, the minimum to sustain irrigation, has been reached. Our objective was to use the mechanistic model ENWATBAL to evaluate the daily and annual water balance for three scenarios of rainfall in this region, a dry (189 mm), an average (449 mm) and a wet (669 mm) year. These three scenarios were applied to two major soil series of this region, Pullman and Amarillo. In all simulations, we used hourly input weather data for a location near Lubbock, Texas and used measured soil hydraulic properties to simulate the water balance for each soil series and the three rainfall scenarios. Results showed that in years with average and wet rain, storage of rainfall occurred in the Pullman but not in in the Amarillo soil series. However, storage of water could be enhanced by combining furrow dikes with minimum tillage along with crop covers that provide a surface residue. The implications of our results for dryland crop production in the semiarid climate of the THP suggest that for years with average and wetter rainfall soils in the Pullman series could store water that would be available for crop use. However, this was not the case for the Amarillo soil series and these soils represent a higher risk for dryland crop production.

The Stem Heat Balance Method to Measure Transpiration: Evaluation of a New Sensor

The measurement of crop transpiration (Tcrop) under field conditions and throughout the growing season is difficult to obtain. An available method uses stem flow gauge sensors, based on the conservation of energy and mass, where the calculated sap flow (F) is a direct measure of Tcrop. This method has been extensively tested on agronomic, horticultural, ornamental aspects and tree crops and the general consensus is that F is a measure of Tcrop. A new sap flow gauge (EXO-SkinTM Sap Flow) sensor, with different placement and number of thermocouples, compared to the original sensor, was introduced, resulting in a different energy balance equation to calculate F. Our objective was to compare values of Tcrop obtained with the new sensor on cotton (Gossypium hirsutum, L) plants to values measured with lysimeters. For this purpose, cotton plants were grown in 11-liter pots in a greenhouse experiment and hourly and daily values of Tcrop were compared for eight days. We used linear regression analysis to compare the hourly and daily values of Tcrop measured with the sensor to corresponding values measured with lysimeters on the same plant. Using a t-test (p > 0.05) we tested if the slope of the line was significantly different than 1 and if the intercept was significantly different than 0. This test indicated that there were no statistical differences between hourly and daily values of Tcrop measured with the new sensor and with the lysimeters. The main advantage of the new sensor is the flexibility of the new heater, allowing for better thermal contact between the plant stem and the temperature sensors. Further, the new sensor requires less wiring and copper connectors, and the number of channels used in a datalogger to record the output from the sensor is reduced by 25%. We conclude that the new sensor correctly measures Tcrop and that additional experiments with field grown plants are required to test the sensor at higher values of Tcrop.

Short Communication: Analysis of Grain Size Distribution through Image Analysis

Herein we describe an approach to measure the volume and to characterize the volume distribution of large numbers of individual small regularly shaped seeds. The results of a preliminary investigation into the distribution of seed size of a multi-seeded sorghum mutant, as compared to the wild type from which it was developed are also reported, and are used as an example of the method’s utility.

Relating Xylem Cavitation to Gas Exchange in Cotton

Acoustic emissions (AEs) from xylem cavitation events are characteristic of transpiration processes. Though a body of work exists describing AEs and limited stem hydraulic conductivity under water stress, there is limited information about the effects of AEs on stomatal aperture and limitation on carbon assimilation. The objective of this work was to relate AEs to drought stress in cotton. Cotton was grown in mini-lysimeters in the greenhouse and instrumented with a portable photosynthesis system and ultrasonic transducers connected to a digital signal-processing unit. Whole plant transpiration, leaf level gas exchange and ultrasonic AEs were measured. Xylem cavitation events temporally associated with the onset of drought stress. The results are consistent with stomatal closure in response to reduced hydraulic conductance from xylem cavitation events. Clear direct empirical evidence of a reduction in carbon assimilation associated with xylem cavitation resulting from water stress is presented.

Systematic Errors Introduced into Sorghum Grain Yield Data: Does the Multiseed (<i>msd</i>) Trait Increase Sorghum Seed Yield?

Multiseed (msd) mutant sorghum [Sorghum bicolor (L.) Moench] lines with greatly increased seed numbers were developed. It was originally thought that the msd trait could increase grain yield several times in comparison with the wild type from which the mutant was derived. However, in a small plot trial, msd seed yield decreased when compared to the parent line. Herein we report results that msd seed yield remained either unchanged or slightly increased in comparison to the parent line. We suggest that attempts to measure msd sorghum seed yield were complicated due to systematic errors associated with the post-harvest processing methods, including threshing and pneumatic winnowing equipment that was used for harvest. That is, seed recovery and seed loss from individual panicles were affected by the post-harvest processing. When evaluating sorghum grain yield of types with different seed sizes, threshing and seed cleaning harvesting methods should be optimized for each sorghum line.

Description of a Novel Allelic “Thick Leafed” Mutant of Sorghum

An allelic sorghum [Sorghum bicolor (L.) Moench] mutant with thick and narrow erect leaves (Thl) and reduced adaxial stomatal density was isolated from the Annotated Individually pedigreed Mutagenized Sorghum mutant library developed at the Plant Stress and Germplasm Development Unit at Lubbock TX. The mutant, Thl, was isolated from a pedigreed M3 family generated by ethyl methanesulfonate mutagenization from an elite inbred sorghum line, BTx623, which had been used to sequence the sorghum genome. The mutant has been backcrossed to the wild-type BTx623 confirming that the trait results derive from a stable recessive nuclear gene mutation. Herein, we briefly described morphological and selected physiological characteristics of this mutant sorghum.

Evaluation of a Metabolic Cotton Seedling Emergence Model

A model for cotton seedling emergence (MaGi) based on malate synthase kinetics was evaluated. Cotton seeds were planted through the early spring and into typical planting times for the areas. Soil temperatures at seed depth were used as inputs into MaGi and a commonly used seedling emergence model based on heat unit accumulation (DD60). Time to 50% emergence was calculated and compared with predicted emergence using MaGi and DD60. MaGi yielded predictive capability without the need to resort to lengthy experimentation required by traditional methods. The results suggest that a physiological or semi-empirical approach incorporating both enzyme kinetics and whole plant temperature responses would be useful for rapidly constructing seedling emergence models.

Research Note: Bird-Resistant Pollination Bags for Sorghum Breeding and Germplasm Maintenance

Bird damage is a problem in sorghum breeding and germplasm maintenance operations. Paper pollination bags are damaged by rain and provide only a minimal deterrent to birds. To overcome these limitations we fabricated pollination bags from spun polyethylene fiber sheeting. No seed yield difference was found between plants bagged with either spun polyethylene or paper. Seed loss by bird damage was nearly eliminated under the polyethylene bags. In areas where bird damage is problematic bird resistant pollination bags can allow for a reduction in the plot size required for breeding and germplasm maintenance operations, increase the productivity of such operations as genetic diversity per unit land area, and make direct measurement of seed yield possible in agronomic field experiments.

Measurement of Cotton Transpiration

There are a few field methods available to directly measure water evapotranspiration (ET) along with its two components, evaporation from the soil (E) and from the crop (T). One such technique that measures T, uses sensors to calculate the sap flow (F) of water through the plant stem and is based on the conservation of mass and energy, i.e., the stem heat balance method. This instrument consists of a flexible heater that is wrapped around the plant stem with temperature sensors to measure the difference in temperature of F below and above the heater. This is a null method, where all inputs and outputs are known and the calculated F is a direct measure of T. This method has been used to measure T in a variety of crops, including cotton, grapes, olive trees, soybean, ornamental and horticultural crops. A new version of the EXO-SkinTM is the Stem Gauge Dual Channel Design (SGDCTM), which was commercially introduced and had a radically new design resulting in a different energy balance, compared to the original design, which needed experimental verification. An initial evaluation was done with potted cotton (Gossypium hirsutum, L.) plants in a greenhouse experiment showing that values of cotton-T measured with the new sensor were accurate;however, this comparison was limited to daily T T in the 2 - 7 mm/d range, representative of the semiarid Texas High Plains. For this purpose, cotton was planted on 12 June 2017 on a 1000 m2 plot in a soil classified in the Amarillo series at the facilities of the USDA-ARS, Lubbock, TX. For a period of 15 days, 2 to 16 Sep 2017, we measured hourly cotton-T with the new sensors and with portable growth chambers (0.75 m × 1 m cross-section, and 1 m height) where water vapor flux was measured at a 10 Hz frequency using an infrared gas analyzer. We used three chambers and, in each chamber, the new sensors were installed on four cotton plants. We used linear regression analysis to compare hourly and daily values of cotton-T measured with the sap flow gauges against T measured by the chambers. Using a t-test (p T for a wide range of environmental conditions.

Annual Rainfall and Dryland Cotton Lint Yield—Southern High Plains of Texas

Agriculture in the Texas High Plains (THP) is in a transition phase of producing crops with a diminishing supply of irrigation-water from the Ogallala aquifer to dryland production systems. This shift is driven by the fact that the depth to the water table of the Ogallala aquifer continues to increase. Dryland cotton production systems are prevalent in the southern counties of the THP and our purpose was to use the long-term dryland cotton lint yields from these counties as precursors of the future cotton production patterns that will emerge in this region. For this purpose, from 1972 to 2018, we calculated the ratio of dryland cotton lint yield per unit of annual rainfall at the county level. This ratio is called crop water productivity (CWP) and has units of mass per unit volume (g/m3). In our analysis, we used cotton lint yield data provided by the National Agricultural Statistics and rainfall data provided by the National Oceanic and Atmospheric Administration. Our results indicated that the three datasets used in our analysis, i.e., cotton lint yield, rainfall and CWP were all normally distributed. In this time period, 1972 to 2018, only one year 2011—a year with a record drought of 179 mm of rain failed to produce a dryland cotton crop in all the counties used in our analysis. The mean cotton lint yield ± standard deviation ranged from a high of 400 ± 175 kg/ha in Lubbock County to a low of 252 ± 144 kg/ha in Andrews County. However, the counties with the largest CWP > 90 g/m3 were Glasscock, Midland and Martin County. The importance of this result is that these counties are in the southern region of the THP and are subject to extreme environmental conditions and yet cotton producers manage to produce a cotton crop in most years. We conclude that management production methods used by these dryland producers represent the future schemes that will need to be adopted in other counties to sustain the emerging dryland cropping systems across the THP.

A Portable and Mobile Rainfall Simulator

An existing Purdue-type rainfall simulator (RFS) was designed to be transported using a commercial flatbed trailer that was modified by cutting out a section of the wooden bed allowing the RFS to be positioned directly above the target area to measure soil erosion and water runoff. To allow water to pass through the trailer undisturbed, the axle of the trailer was removed and replaced with axle-free wheel hubs and springs. Additionally, a remote control mover rated for 2000 kg was incorporated in our design. The final result was a portable and mobile RFS that can be moved to fields using a trailer hitch and can be operated by two individuals. The cost of the hardware, not including the RFS, is $5300 (USD) and detailed design plans are available.