Strontium-substituted bioactive glass(Sr-BG)has shown superior performance in bone regeneration.Sr-BG-induced osteogenesis has been extensively studied;however,Sr-BG-mediated osteoclastogenesis and the underlying mole...Strontium-substituted bioactive glass(Sr-BG)has shown superior performance in bone regeneration.Sr-BG-induced osteogenesis has been extensively studied;however,Sr-BG-mediated osteoclastogenesis and the underlying molecular mechanism remain unclear.It is recognized that the balance of osteogenesis and osteoclastogenesis is closely related to bone repair,and the receptor activators of nuclear factor kappaB ligand(RANKL)signaling pathway plays a key role of in the regulation of osteoclastogenesis.Herein,we studied the potential impact and underling mechanism of strontium-substituted sub-micron bioactive glass(Sr-SBG)on RANKL-induced osteoclast activation and differentiation in vitro.As expected,Sr-SBG inhibited RANKL-mediated osteoclastogenesis significantly with the experimental performance of decreased mature osteoclasts formation and downregulation of osteoclastogenesis-related gene expression.Furthermore,it was found that Sr-SBG might suppress osteoclastogenesis by the combined effect of strontium and silicon released through inhibition of RANKL-induced activation of p38 and NF-κB pathway.These results elaborated the effect of Sr-SBG-based materials on osteoclastogenesis through RANKLinduced downstream pathway and might represent a significant guidance for designing better bone repair materials.展开更多
基金supported by Natural Science Foundation of Guangdong Province of China(No.2019A1515010365)National Natural Science Foundation of China(Grant Nos U1501245 and 51672088)+1 种基金China Postdoctoral Science Foundation(2018M633065)Scientific Research Cultivation Fund for Young Teachers of South China Normal University(18KJ16).
文摘Strontium-substituted bioactive glass(Sr-BG)has shown superior performance in bone regeneration.Sr-BG-induced osteogenesis has been extensively studied;however,Sr-BG-mediated osteoclastogenesis and the underlying molecular mechanism remain unclear.It is recognized that the balance of osteogenesis and osteoclastogenesis is closely related to bone repair,and the receptor activators of nuclear factor kappaB ligand(RANKL)signaling pathway plays a key role of in the regulation of osteoclastogenesis.Herein,we studied the potential impact and underling mechanism of strontium-substituted sub-micron bioactive glass(Sr-SBG)on RANKL-induced osteoclast activation and differentiation in vitro.As expected,Sr-SBG inhibited RANKL-mediated osteoclastogenesis significantly with the experimental performance of decreased mature osteoclasts formation and downregulation of osteoclastogenesis-related gene expression.Furthermore,it was found that Sr-SBG might suppress osteoclastogenesis by the combined effect of strontium and silicon released through inhibition of RANKL-induced activation of p38 and NF-κB pathway.These results elaborated the effect of Sr-SBG-based materials on osteoclastogenesis through RANKLinduced downstream pathway and might represent a significant guidance for designing better bone repair materials.
