Inflammatory bowel disease(IBD)is characterized by recurrent attacks and long courses,and the number of patients has expanded rapidly year by year.Additionally,current conventional strategies exist serious adverse eff...Inflammatory bowel disease(IBD)is characterized by recurrent attacks and long courses,and the number of patients has expanded rapidly year by year.Additionally,current conventional strategies exist serious adverse effects.In this case,it is an urgent issue to find out an effective and safe treatment.Functional oligosaccharides possess safe and excellent physiological activities,and have attracted enormous attention due to their great therapeutic potential for IBD.This review emphasizes the attenuating effects of distinct functional oligosaccharides on IBD and their structure,and summarizes the main mechanisms from the aspects of regulating intestinal fl ora structure,repairing intestinal barrier,modulating immune function and mediating related signaling pathways in order to reveal the relationship between functional oligosaccharides,immune regulation,intestinal epithelial cells,gut fl ora and IBD treatment.Oligosaccharides possess excellent protective effects on IBD,and can be considered as safe and functional ingredients in the health food and pharmaceutical industry.展开更多
Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been rep...Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been reported.In this study,we used bioinformatics to determine the role and mechanisms of EE in inhibiting breast cancer(BRCA),prostate cancer(PRAD),lung cancer(LUAD),and bladder cancer(BLCA).Finally,the MTT method was used to evaluate the inhibitory effect of EE on breast cancer MDA-MB-231 cells,and the target genes were determined by qRT-PCR.The results showed that the survival rate of MDA-MB-231 cells was reduced by 27.33%after 400μg/mL EE treatment.The results of qRT-PCR showed that EE down-regulated target genes in breast cancer cells,which was consistent with our predicted results.Moreover,our results indicated that EE may have impact on the transcription and translation of BLCA cells by targeting ADCY2,ADCY5 and JUN.In BRCA,we identified three targets for EE.In addition,EE affected DNA repair and the development of BRCA by affecting the expression of RAD51.In LUAD,we identified 9 targets for EE,8 of which were related to DNA repair.EE inhibited PRAD by targeting ADCY8.Our research was the first report on the targets and mechanisms of EE for suppressing cancer combined with clinical data.Our research also provides theoretical support for using EE to suppress cancer.展开更多
基金financially supported by Sichuan Science and Technology Program[2021YFSY0035]Heilongjiang Touyan Team[HITTY-20190034].
文摘Inflammatory bowel disease(IBD)is characterized by recurrent attacks and long courses,and the number of patients has expanded rapidly year by year.Additionally,current conventional strategies exist serious adverse effects.In this case,it is an urgent issue to find out an effective and safe treatment.Functional oligosaccharides possess safe and excellent physiological activities,and have attracted enormous attention due to their great therapeutic potential for IBD.This review emphasizes the attenuating effects of distinct functional oligosaccharides on IBD and their structure,and summarizes the main mechanisms from the aspects of regulating intestinal fl ora structure,repairing intestinal barrier,modulating immune function and mediating related signaling pathways in order to reveal the relationship between functional oligosaccharides,immune regulation,intestinal epithelial cells,gut fl ora and IBD treatment.Oligosaccharides possess excellent protective effects on IBD,and can be considered as safe and functional ingredients in the health food and pharmaceutical industry.
基金This research was supported by The National Key Research and Development Program of China(2017YFC1601900).
文摘Eleutheroside E(EE)is a monomer compound isolated from Acanthopanax senticosus,which has functions of antifatigue,antioxidation,and immune regulation.However,the function of EE in inhibiting cancer has rarely been reported.In this study,we used bioinformatics to determine the role and mechanisms of EE in inhibiting breast cancer(BRCA),prostate cancer(PRAD),lung cancer(LUAD),and bladder cancer(BLCA).Finally,the MTT method was used to evaluate the inhibitory effect of EE on breast cancer MDA-MB-231 cells,and the target genes were determined by qRT-PCR.The results showed that the survival rate of MDA-MB-231 cells was reduced by 27.33%after 400μg/mL EE treatment.The results of qRT-PCR showed that EE down-regulated target genes in breast cancer cells,which was consistent with our predicted results.Moreover,our results indicated that EE may have impact on the transcription and translation of BLCA cells by targeting ADCY2,ADCY5 and JUN.In BRCA,we identified three targets for EE.In addition,EE affected DNA repair and the development of BRCA by affecting the expression of RAD51.In LUAD,we identified 9 targets for EE,8 of which were related to DNA repair.EE inhibited PRAD by targeting ADCY8.Our research was the first report on the targets and mechanisms of EE for suppressing cancer combined with clinical data.Our research also provides theoretical support for using EE to suppress cancer.
