The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant,they are also important in their ornamental appeal in Narcissus plant.I...The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant,they are also important in their ornamental appeal in Narcissus plant.In this study,we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor.Comparative transcriptome analysis of lossand gain-of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways.Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS,NtLAR,and NtDFR that were cloned by genome walking assay,and activated NtFLS and NtLAR expression but repressed NtDFR expression.More interestingly,NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptomebased WGCNA and confirmed by yeast two hybrid,bimolecular fluorescence complementation and coimmunoprecipitation assay.Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted(pro)anthocyanin accumulation,while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols,but repressed NtDFR expression.These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and(pro)anthocyanin biosynthesis.展开更多
Dear Editor ：The sustainable cultivation of rice, which serves as staple food crop for more than half of the world＇s population, is under serious threat due to the huge yield losses inflicted by rice blast disease c...Dear Editor ：The sustainable cultivation of rice, which serves as staple food crop for more than half of the world＇s population, is under serious threat due to the huge yield losses inflicted by rice blast disease caused by the globally destructive fungus Magnaporthe oryzae （Pyricularia oryzae） （Dean et al., 2012; Nalley et al., 2016; Deng et al., 2017）. This filamentous ascomycete fungus is also capable of causing blast infection on other economically important cereal crops, including wheat, millet, and barley, making it the world＇s most important plant pathogenic fungus （Zhong et al., 2016）.展开更多
Breast cancer is one of the most common malignancies that seriously threaten women’s health.In the process of the malignant transformation of breast cancer,metabolic reprogramming and immune evasion represent the two...Breast cancer is one of the most common malignancies that seriously threaten women’s health.In the process of the malignant transformation of breast cancer,metabolic reprogramming and immune evasion represent the two main fascinating characteristics of cancer and facilitate cancer cell proliferation.Breast cancer cells generate energy through increased glucose metabolism.Lipid metabolism contributes to biological signal pathways and forms cell membranes except energy generation.Amino acids act as basic protein units and metabolic regulators in supporting cell growth.For tumor-associated immunity,poor immunogenicity and heightened immunosuppression cause breast cancer cells to evade the host’s immune system.For the past few years,the complex mechanisms of metabolic reprogramming and immune evasion are deeply investigated,and the genes involved in these processes are used as clinical therapeutic targets for breast cancer.Here,we review the recent findings related to abnormal metabolism and immune characteristics,regulatory mechanisms,their links,and relevant therapeutic strategies.展开更多
The brain-computer interface(BCI)technology has received lots of attention in the field of scientific research because it can help disabled people improve their quality of life.Steady-state visual evoked potential(SSV...The brain-computer interface(BCI)technology has received lots of attention in the field of scientific research because it can help disabled people improve their quality of life.Steady-state visual evoked potential(SSVEP)is the most researched BCI experimental paradigm,which offers the advantages of high signal-to-noise ratio and short training-time requirement by users.In a complete BCI system,the two most critical components are the experimental paradigm and decoding algorithm.However,a systematic combination of the SSVEP experimental paradigm and decoding algorithms is missing in existing studies.In the present study,the transient visual evoked potential,SSVEP,and various improved SSVEP paradigms are compared and analyzed,and the problems and development bottlenecks in the experimental paradigm are finally pointed out.Subsequently,the canonical correlation analysis and various improved decoding algorithms are introduced,and the opportunities and challenges of the SSVEP decoding algorithm are discussed.展开更多
Let c>1 and 0<γ<1.We study the solubility of the Diophantine inequality∣p^(c)_(1)+p^(c)_(2)+…+p^(c)_(s)−N∣<(log N)^(−1) in Piatetski-Shapiro primes p_(1),p_(2),…,p_(s) of the form pj=[m^(1/γ)]for som...Let c>1 and 0<γ<1.We study the solubility of the Diophantine inequality∣p^(c)_(1)+p^(c)_(2)+…+p^(c)_(s)−N∣<(log N)^(−1) in Piatetski-Shapiro primes p_(1),p_(2),…,p_(s) of the form pj=[m^(1/γ)]for some m∈ℕ,and improve the previous results in the cases s=2,3,4.展开更多
Breast cancer is one of the most prevalent cancers worldwide and a major cause of cancer-related mortality in women.Although recent breast cancer therapies,including surgery,radiotherapy,chemotherapy,targeted therapy,...Breast cancer is one of the most prevalent cancers worldwide and a major cause of cancer-related mortality in women.Although recent breast cancer therapies,including surgery,radiotherapy,chemotherapy,targeted therapy,and endocrine therapy,have substantially improved patient survival,the cure rate is still low.Understanding the molecular mechanisms underlying breast cancer development.展开更多
基金Open access funding provided by Shanghai Jiao Tong Universitysupported by the“100 talent scientist plan”program of Fujian Province of China to Ying Miao.
文摘The color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant,they are also important in their ornamental appeal in Narcissus plant.In this study,we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor.Comparative transcriptome analysis of lossand gain-of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways.Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS,NtLAR,and NtDFR that were cloned by genome walking assay,and activated NtFLS and NtLAR expression but repressed NtDFR expression.More interestingly,NtMYB12 can interact with NtbHLH1 and NtWD40-1 proteins via R3 domain that were selected by transcriptomebased WGCNA and confirmed by yeast two hybrid,bimolecular fluorescence complementation and coimmunoprecipitation assay.Interaction of NtMYB12 with NtbHLH1 and NtWD40-1 forming MYB-bHLH-WD40 triplex specially activated NtDFR and NtANS expression and promoted(pro)anthocyanin accumulation,while NtMYB12 alone activated NtFLS and NtLAR expression and accumulated flavonols,but repressed NtDFR expression.These results indicated that NtMYB12 alone or NtMYB12-bHLH1-WD40-1 triplex requires for competition of metabolism fluxes between flavonol and(pro)anthocyanin biosynthesis.
文摘Dear Editor ：The sustainable cultivation of rice, which serves as staple food crop for more than half of the world＇s population, is under serious threat due to the huge yield losses inflicted by rice blast disease caused by the globally destructive fungus Magnaporthe oryzae （Pyricularia oryzae） （Dean et al., 2012; Nalley et al., 2016; Deng et al., 2017）. This filamentous ascomycete fungus is also capable of causing blast infection on other economically important cereal crops, including wheat, millet, and barley, making it the world＇s most important plant pathogenic fungus （Zhong et al., 2016）.
文摘Breast cancer is one of the most common malignancies that seriously threaten women’s health.In the process of the malignant transformation of breast cancer,metabolic reprogramming and immune evasion represent the two main fascinating characteristics of cancer and facilitate cancer cell proliferation.Breast cancer cells generate energy through increased glucose metabolism.Lipid metabolism contributes to biological signal pathways and forms cell membranes except energy generation.Amino acids act as basic protein units and metabolic regulators in supporting cell growth.For tumor-associated immunity,poor immunogenicity and heightened immunosuppression cause breast cancer cells to evade the host’s immune system.For the past few years,the complex mechanisms of metabolic reprogramming and immune evasion are deeply investigated,and the genes involved in these processes are used as clinical therapeutic targets for breast cancer.Here,we review the recent findings related to abnormal metabolism and immune characteristics,regulatory mechanisms,their links,and relevant therapeutic strategies.
基金supported by the National Natural Science Foundation of China(Grant Nos.U20A20191,61727807,82071912,12104049)the Beijing Municipal Science&Technology Commission(Grant No.Z201100007720009)+4 种基金the Fundamental Research Funds for the Central Universities(Grant No.2021CX11011)the China Postdoctoral Science Foundation(Grant No.2020TQ0040)the National Key Research and Development Program of China(Grant No.2020YFC2007305)the BIT Research and Innovation Promoting Project(Grant No.2022YCXZ026)the Ensan Foundation(Grant No.2022026)。
文摘The brain-computer interface(BCI)technology has received lots of attention in the field of scientific research because it can help disabled people improve their quality of life.Steady-state visual evoked potential(SSVEP)is the most researched BCI experimental paradigm,which offers the advantages of high signal-to-noise ratio and short training-time requirement by users.In a complete BCI system,the two most critical components are the experimental paradigm and decoding algorithm.However,a systematic combination of the SSVEP experimental paradigm and decoding algorithms is missing in existing studies.In the present study,the transient visual evoked potential,SSVEP,and various improved SSVEP paradigms are compared and analyzed,and the problems and development bottlenecks in the experimental paradigm are finally pointed out.Subsequently,the canonical correlation analysis and various improved decoding algorithms are introduced,and the opportunities and challenges of the SSVEP decoding algorithm are discussed.
基金The authors would like to express their gratitude to the referee for his or her careful reading and valuable suggestionsThis work was supported by the National Natural Science Foundation of China(Grant Nos.11771256,11971476).
文摘Let c>1 and 0<γ<1.We study the solubility of the Diophantine inequality∣p^(c)_(1)+p^(c)_(2)+…+p^(c)_(s)−N∣<(log N)^(−1) in Piatetski-Shapiro primes p_(1),p_(2),…,p_(s) of the form pj=[m^(1/γ)]for some m∈ℕ,and improve the previous results in the cases s=2,3,4.
基金supported by the National Key Research and Development Program of China(2017YFA0505602)the National Natural Science Foundation of China(81930078 and 81872246)。
文摘Breast cancer is one of the most prevalent cancers worldwide and a major cause of cancer-related mortality in women.Although recent breast cancer therapies,including surgery,radiotherapy,chemotherapy,targeted therapy,and endocrine therapy,have substantially improved patient survival,the cure rate is still low.Understanding the molecular mechanisms underlying breast cancer development.
