Recently,biosynthesis of silver nanoparticles using bacteria,fungus and plants has emerged as a simple and viable alternative to more complex physical and chemical synthetic procedures.The present investigation explai...Recently,biosynthesis of silver nanoparticles using bacteria,fungus and plants has emerged as a simple and viable alternative to more complex physical and chemical synthetic procedures.The present investigation explains rapid and extracellular synthesis of silver nanoparticles using fungus Fusarium oxysporum NGD and characterization of the synthesized silver nanoparticles using UV-Vis spectroscopy,scanning electron microscopy,energy dispersive spectroscopy and X-ray diffraction analysis.The size range of the synthesized silver nanoparticles was around 16.3e70 nm.The FTIR studies showed major peaks of proteins involved in the synthesis of silver nanoparticles.Further,antibacterial effect of the silver nanoparticles against multidrug resistant pathogens Enterobacter sp.ANT 02[HM803168],Pseudomonas aeruginosa ANT 04[HM803170],Klebsiella pneumoniae ANT 03[HM803169]and Escherichia coli ANT 01[HM803167]was tested using turbidometric assay at 10,20,30,40 mg AgNPs/ml alone and in combination with ampicillin using agar well diffusion assay.All the resistant bacteria were found to be susceptible to the antibiotic in the presence of the silver nanoparticles.展开更多
Objective:To reveal the screening of metal tolerance and dye-decolorizing of Nocardiopsis sp.Methods:NiSO_(4) and Congo red dye were used for evaluating the metal tolerance and dyedecolorizing of the randomly selected...Objective:To reveal the screening of metal tolerance and dye-decolorizing of Nocardiopsis sp.Methods:NiSO_(4) and Congo red dye were used for evaluating the metal tolerance and dyedecolorizing of the randomly selected actinobacterial isolates.Results:Nocardiopsis sp.SD8 showed a better efficiency in Ni(II)tolerance,though a longer lag phase was observed for this microorganism grown for 7 days in integrated mismatch negativity.Interestingly,we also found that Nocardiopsis sp.SD8 had dye-decolorizing,hemolytic,lipase and protease activity.Conclusions:The present results revealed the bioremediation of metal resistant and diverse properties of Nocardiopsis sp.SD8 and further investigations are needed to extract and identify the potent molecule.展开更多
基金the Department of Science and Technology,Government of India,for providing the financial assistance in the form of Research Fellowship under the DST e Promotion of University Research and Scientific Excellence(PURSE)scheme(Ref.No.41891/E8/2010 dated 12.12.11)to P.M.GopinathUniversity Grants Commission,Government of India for providing financial assistance(Ref.No.41-1135/2012(SR)dated 26.06.2012).
文摘Recently,biosynthesis of silver nanoparticles using bacteria,fungus and plants has emerged as a simple and viable alternative to more complex physical and chemical synthetic procedures.The present investigation explains rapid and extracellular synthesis of silver nanoparticles using fungus Fusarium oxysporum NGD and characterization of the synthesized silver nanoparticles using UV-Vis spectroscopy,scanning electron microscopy,energy dispersive spectroscopy and X-ray diffraction analysis.The size range of the synthesized silver nanoparticles was around 16.3e70 nm.The FTIR studies showed major peaks of proteins involved in the synthesis of silver nanoparticles.Further,antibacterial effect of the silver nanoparticles against multidrug resistant pathogens Enterobacter sp.ANT 02[HM803168],Pseudomonas aeruginosa ANT 04[HM803170],Klebsiella pneumoniae ANT 03[HM803169]and Escherichia coli ANT 01[HM803167]was tested using turbidometric assay at 10,20,30,40 mg AgNPs/ml alone and in combination with ampicillin using agar well diffusion assay.All the resistant bacteria were found to be susceptible to the antibiotic in the presence of the silver nanoparticles.
基金Supported by DST(Ref No.DST/IS-STAC/CO2-SR-163/13(G)).
文摘Objective:To reveal the screening of metal tolerance and dye-decolorizing of Nocardiopsis sp.Methods:NiSO_(4) and Congo red dye were used for evaluating the metal tolerance and dyedecolorizing of the randomly selected actinobacterial isolates.Results:Nocardiopsis sp.SD8 showed a better efficiency in Ni(II)tolerance,though a longer lag phase was observed for this microorganism grown for 7 days in integrated mismatch negativity.Interestingly,we also found that Nocardiopsis sp.SD8 had dye-decolorizing,hemolytic,lipase and protease activity.Conclusions:The present results revealed the bioremediation of metal resistant and diverse properties of Nocardiopsis sp.SD8 and further investigations are needed to extract and identify the potent molecule.
