Objective:To analyse experimental infection and immune system of shrimp(Penaeus monodon)against Vibrios furnissii(V.furnissii).Methods:Experimental animals were collected and acclimatized by maintaining specific tempe...Objective:To analyse experimental infection and immune system of shrimp(Penaeus monodon)against Vibrios furnissii(V.furnissii).Methods:Experimental animals were collected and acclimatized by maintaining specific temperature,pH and salinity to avoid mortality.Shrimps were experimentally infected with V.furnissii and their immune responses were monitored.After the infection all the shrimps were monitored for any symptoms,death rate in 0,12,24,36,48 h.Then haemolymph were collected and tetrahydrocannabinol,phenol oxidase,nitroblue tetrazolium and lysozyme were monitored in every 12 h at the interval of 48 h.Results:Shrimps infected by live V.furnissii had showed gradual increase in tetrahydrocannabinol,phenol oxidase activity,nitro-blue-tetrazolium and lysozyme activity comparing with the killed and control.Conclusions:The live V.furnissii had showed infection in the shrimp immune system.The live V.furnissii shows infection in experimental shrimps comparing with killed V.furnissii.So the V.furnissii in nature cause the infection in shrimp Penaeus monodon immune system.This report could be applied to control of the infection in shrimp hatchery.展开更多
基金Supported by University Grant Commission(UGC),New Delhi,India.
文摘Objective:To analyse experimental infection and immune system of shrimp(Penaeus monodon)against Vibrios furnissii(V.furnissii).Methods:Experimental animals were collected and acclimatized by maintaining specific temperature,pH and salinity to avoid mortality.Shrimps were experimentally infected with V.furnissii and their immune responses were monitored.After the infection all the shrimps were monitored for any symptoms,death rate in 0,12,24,36,48 h.Then haemolymph were collected and tetrahydrocannabinol,phenol oxidase,nitroblue tetrazolium and lysozyme were monitored in every 12 h at the interval of 48 h.Results:Shrimps infected by live V.furnissii had showed gradual increase in tetrahydrocannabinol,phenol oxidase activity,nitro-blue-tetrazolium and lysozyme activity comparing with the killed and control.Conclusions:The live V.furnissii had showed infection in the shrimp immune system.The live V.furnissii shows infection in experimental shrimps comparing with killed V.furnissii.So the V.furnissii in nature cause the infection in shrimp Penaeus monodon immune system.This report could be applied to control of the infection in shrimp hatchery.