The electrical and thermal characterization of near-surface electrical discharge plasma driven by radio frequency voltage are investigated experimentally in this paper. The influences of operating pressure, electrode ...The electrical and thermal characterization of near-surface electrical discharge plasma driven by radio frequency voltage are investigated experimentally in this paper. The influences of operating pressure, electrode distance, and duty cycle on the discharge are studied. When pressure reaches 60 Ton. （1 Torr= 1.33322 x 102 Pa） the transition from diffuse glow mode to constricted mode occurs. With the operating pressure varying from 10 Tort to 60 Torr, the discharge energy calculated from the charge-voltage （Q-V） Lissajous figure decreases rapidly, while it remains unchanged between 60 Torr and 460 Torr. Under certain experimental conditions, there exists an optimized electrode distance （8 mm）. As the duty cycle of applied voltage increases, the voltage-current waveforms and Q-V Lissajous figures show no distinct changes.展开更多
This study aims to conduct a cost-effectiveness analysis of three different anesthesia strategies,namely chatting while under local anesthesia(Chat-LA),total intravenous anesthesia(TIVA),and general anesthesia with la...This study aims to conduct a cost-effectiveness analysis of three different anesthesia strategies,namely chatting while under local anesthesia(Chat-LA),total intravenous anesthesia(TIVA),and general anesthesia with laryngeal mask airway(GA-LMA),employed in transperineal magnetic resonance imaging(MRI)/ultrasound(US)fusion prostate biopsy(TP-MUF-PB).A retrospective study was conducted involving 1202 patients who underwent TP-MUF-PB from June 2016 to April 2023 at The First Affiliated Hospital of Soochow University(Suzhou,China).Clinical data and outcomes,including total costs,complications,and quality-adjusted life years(QALYs),were compared.Probability sensitivity and subgroup analyses were also performed.Chat-LA was found to be the most cost-effective option,outperforming both TIVA and GA-LMA.However,subgroup analyses revealed that in younger patients(under 65 years old)and those with smaller prostate volumes(<40 ml),TIVA emerged as a more cost-effective strategy.While Chat-LA may generally be the most cost-effective and safer anesthesia method for TP-MUF-PB,personalization of anesthesia strategies is crucial,considering specific patient demographics such as age and prostate volume.展开更多
In order to solve the problem of the difficulty of igniting and steadily propagating a continuous rotating detonation engine when using liquid hydrocarbon fuel, an experiment was carried out using a dielectric barrier...In order to solve the problem of the difficulty of igniting and steadily propagating a continuous rotating detonation engine when using liquid hydrocarbon fuel, an experiment was carried out using a dielectric barrier discharge excited by a nanosecond power supply to crack n-decane, the single alternative fuel to aviation kerosene, in a pre-heated argon environment.By changing the voltages and the discharge frequencies, the concentrations of different components as well as a number of different species were acquired.The generating mechanism of olefins and alkanes together with their competition mechanism were acquired.The influence of the voltage on isomer products was also analyzed.The results demonstrate that the bond energy distribution and the species generating condition are the main factors affecting the formation of the products.With the increasing of voltage and discharge frequency, small molecule olefins, large molecular olefins, large molecular alkanes, small molecular alkanes, and hydrogen were detected, and in turn, their concentrations were also increased except for ethylene;what is more, when the voltage was increased over 8.5 kV, the n-butene converted to trans-butene, and the n-pentene converted to isoamylene.展开更多
Background:μ-opioid receptor agonists(MORAs)are indispensable for analgesia in bladder cancer(BC)patients,both during surgery and for chronic pain treatment.Whether MORAs affect BC progression and metastasis remains ...Background:μ-opioid receptor agonists(MORAs)are indispensable for analgesia in bladder cancer(BC)patients,both during surgery and for chronic pain treatment.Whether MORAs affect BC progression and metastasis remains largely unknown.This study focused on the effects of MORAs on the formation of circulating tumor cells(CTCs)in BC and aimed to provide potential therapeutic targets,which would retain the pain-relieving effects of MORAs in BC patients without sacrificing their long-term prognosis.Methods:Different preclinical models were used to identify the effects of MORAs on the progression of BC.A novel immunocapture microfluidic chip was utilized to analyze whether MORAs affected the number of CTCs in mouse models and clinical BC patients.Bioinformatic analyses,total transcriptome sequencing,and molecular biology methods were then used to investigate the underlying mechanisms in these models and in BC cell lines.Results:Mouse models of hematogenous metastasis and in situ BC demonstrated that tumor metastasis was significantly increased after MORA treatment.A significant increase in the number of mesenchymal and/or epithelial CTCs was detected after MORA treatment in both the mouse models and clinical trial patients.Mechanistically,MORAs facilitated the formation of CTCs by activating the MOR/PI3K/AKT/Slug signaling pathway,hereby promoting the epithelialmesenchymal transition(EMT)of BC cells,as knockdown of MOR,Slug or blockade of PI3K inhibited the EMT process and CTC formation.Conclusion:MORAs promoted BC metastasis by facilitating CTC formation.The EMT-CTC axis could be targeted for preventive measures during MORA treatment to inhibit the associated tumormetastasis or recurrence in BC patients.展开更多
Background:Bladder cancer,characterized by a high potential of tumor recurrence,has high lifelong monitoring and treatment costs.To date,tumor cells with intrinsic softness have been identified to function as cancer s...Background:Bladder cancer,characterized by a high potential of tumor recurrence,has high lifelong monitoring and treatment costs.To date,tumor cells with intrinsic softness have been identified to function as cancer stem cells in several cancer types.Nonetheless,the existence of soft tumor cells in bladder tumors remains elusive.Thus,our study aimed to develop a microbarrier microfluidic chip to efficiently isolate deformable tumor cells from distinct types of bladder cancer cells.Methods:The stiffness of bladder cancer cells was determined by atomic force microscopy(AFM).The modified microfluidic chip was utilized to separate soft cells,and the 3D Matrigel culture system was to maintain the softness of tumor cells.Expression patterns of integrinβ8(ITGB8),protein kinase B(AKT),and mammalian target of rapamycin(mTOR)were determined by Western blotting.Double immunostaining was conducted to examine the interaction between F-actin and tripartite motif containing 59(TRIM59).The stem-cell-like characteristics of soft cells were explored by colony formation assay and in vivo studies upon xenografted tumor models.Results:Using our newly designed microfluidic approach,we identified a small fraction of soft tumor cells in bladder cancer cells.More importantly,the existence of soft tumor cells was confirmed in clinical human bladder cancer specimens,in which the number of soft tumor cells was associated with tumor relapse.Furthermore,we demonstrated that the biomechanical stimuli arising from 3D Matrigel activated the F-actin/ITGB8/TRIM59/AKT/mTOR/glycolysis pathways to enhance the softness and tumorigenic capacity of tumor cells.Simultaneously,we detected a remarkable up-regulation in ITGB8,TRIM59,and phospho-AKT in clinical bladder recurrent tumors compared with their non-recurrent counterparts.Conclusions:The ITGB8/TRIM59/AKT/mTOR/glycolysis axis plays a crucial role in modulating tumor softness and stemness.Meanwhile,the soft tumor cells become more sensitive to chemotherapy after stiffening,that offers new insights for hampering tumor progression and recurrence.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11472306,51407197,and 51507187)
文摘The electrical and thermal characterization of near-surface electrical discharge plasma driven by radio frequency voltage are investigated experimentally in this paper. The influences of operating pressure, electrode distance, and duty cycle on the discharge are studied. When pressure reaches 60 Ton. （1 Torr= 1.33322 x 102 Pa） the transition from diffuse glow mode to constricted mode occurs. With the operating pressure varying from 10 Tort to 60 Torr, the discharge energy calculated from the charge-voltage （Q-V） Lissajous figure decreases rapidly, while it remains unchanged between 60 Torr and 460 Torr. Under certain experimental conditions, there exists an optimized electrode distance （8 mm）. As the duty cycle of applied voltage increases, the voltage-current waveforms and Q-V Lissajous figures show no distinct changes.
基金Key Medical Research Projects in Jiangsu Province(No.ZD2022021)the Key Research and Development Program of Jiangsu Province(No.BE2020654).
文摘This study aims to conduct a cost-effectiveness analysis of three different anesthesia strategies,namely chatting while under local anesthesia(Chat-LA),total intravenous anesthesia(TIVA),and general anesthesia with laryngeal mask airway(GA-LMA),employed in transperineal magnetic resonance imaging(MRI)/ultrasound(US)fusion prostate biopsy(TP-MUF-PB).A retrospective study was conducted involving 1202 patients who underwent TP-MUF-PB from June 2016 to April 2023 at The First Affiliated Hospital of Soochow University(Suzhou,China).Clinical data and outcomes,including total costs,complications,and quality-adjusted life years(QALYs),were compared.Probability sensitivity and subgroup analyses were also performed.Chat-LA was found to be the most cost-effective option,outperforming both TIVA and GA-LMA.However,subgroup analyses revealed that in younger patients(under 65 years old)and those with smaller prostate volumes(<40 ml),TIVA emerged as a more cost-effective strategy.While Chat-LA may generally be the most cost-effective and safer anesthesia method for TP-MUF-PB,personalization of anesthesia strategies is crucial,considering specific patient demographics such as age and prostate volume.
基金supported by National Natural Science Foundation of China (Nos.91541120, 91641204)
文摘In order to solve the problem of the difficulty of igniting and steadily propagating a continuous rotating detonation engine when using liquid hydrocarbon fuel, an experiment was carried out using a dielectric barrier discharge excited by a nanosecond power supply to crack n-decane, the single alternative fuel to aviation kerosene, in a pre-heated argon environment.By changing the voltages and the discharge frequencies, the concentrations of different components as well as a number of different species were acquired.The generating mechanism of olefins and alkanes together with their competition mechanism were acquired.The influence of the voltage on isomer products was also analyzed.The results demonstrate that the bond energy distribution and the species generating condition are the main factors affecting the formation of the products.With the increasing of voltage and discharge frequency, small molecule olefins, large molecular olefins, large molecular alkanes, small molecular alkanes, and hydrogen were detected, and in turn, their concentrations were also increased except for ethylene;what is more, when the voltage was increased over 8.5 kV, the n-butene converted to trans-butene, and the n-pentene converted to isoamylene.
基金National Natural Science Foundation of China,Grant/Award Numbers:82171177,82173076Shanghai Science and Technology Committee Foundation,Grant/Award Number:19ZR1430600+6 种基金Clinical Research Plan of Shanghai Hospital Development Center,Grant/Award Number:SHDC2020CR4062Key Specialty Construction Project of Pudong Health and Family Planning Commission of Shanghai,Grant/Award Number:PWZxq2017-06Shanghai Municipal Key Clinical Specialty,Grant/Award Number:shslczdzk03601Shanghai Engineering Research Center of Peri-operative Organ Support and Function Preservation,Grant/Award Number:20DZ2254200Shanghai 2021“Science and Technology Innovation Action Plan”domestic science and technology cooperation project,Grant/Award Number:21015801500Innovative research team of high-level local universities in Shanghai,Grant/Award Number:SHSMU-ZLCX20212601STI2030-Major Projects,Grant/Award Number:2022ZD0206200。
文摘Background:μ-opioid receptor agonists(MORAs)are indispensable for analgesia in bladder cancer(BC)patients,both during surgery and for chronic pain treatment.Whether MORAs affect BC progression and metastasis remains largely unknown.This study focused on the effects of MORAs on the formation of circulating tumor cells(CTCs)in BC and aimed to provide potential therapeutic targets,which would retain the pain-relieving effects of MORAs in BC patients without sacrificing their long-term prognosis.Methods:Different preclinical models were used to identify the effects of MORAs on the progression of BC.A novel immunocapture microfluidic chip was utilized to analyze whether MORAs affected the number of CTCs in mouse models and clinical BC patients.Bioinformatic analyses,total transcriptome sequencing,and molecular biology methods were then used to investigate the underlying mechanisms in these models and in BC cell lines.Results:Mouse models of hematogenous metastasis and in situ BC demonstrated that tumor metastasis was significantly increased after MORA treatment.A significant increase in the number of mesenchymal and/or epithelial CTCs was detected after MORA treatment in both the mouse models and clinical trial patients.Mechanistically,MORAs facilitated the formation of CTCs by activating the MOR/PI3K/AKT/Slug signaling pathway,hereby promoting the epithelialmesenchymal transition(EMT)of BC cells,as knockdown of MOR,Slug or blockade of PI3K inhibited the EMT process and CTC formation.Conclusion:MORAs promoted BC metastasis by facilitating CTC formation.The EMT-CTC axis could be targeted for preventive measures during MORA treatment to inhibit the associated tumormetastasis or recurrence in BC patients.
基金supported by the National Natural Science Foundation of China(Nos.81902578,81974098,8197032158)China Postdoctoral Science Foundation(No.2020M670057ZX)+3 种基金Programs from Science and Technology Department of Sichuan Province(No.2021YJ0462)Post-doctoral Science Research Foundation of Sichuan University(No.2020SCU12041)Post-Doctor Research Project,West China Hospital,Sichuan University(Nos.2018HXBH084,2019HXBH092)the National key research and development program of China(No.2020YFC2008601)
文摘Background:Bladder cancer,characterized by a high potential of tumor recurrence,has high lifelong monitoring and treatment costs.To date,tumor cells with intrinsic softness have been identified to function as cancer stem cells in several cancer types.Nonetheless,the existence of soft tumor cells in bladder tumors remains elusive.Thus,our study aimed to develop a microbarrier microfluidic chip to efficiently isolate deformable tumor cells from distinct types of bladder cancer cells.Methods:The stiffness of bladder cancer cells was determined by atomic force microscopy(AFM).The modified microfluidic chip was utilized to separate soft cells,and the 3D Matrigel culture system was to maintain the softness of tumor cells.Expression patterns of integrinβ8(ITGB8),protein kinase B(AKT),and mammalian target of rapamycin(mTOR)were determined by Western blotting.Double immunostaining was conducted to examine the interaction between F-actin and tripartite motif containing 59(TRIM59).The stem-cell-like characteristics of soft cells were explored by colony formation assay and in vivo studies upon xenografted tumor models.Results:Using our newly designed microfluidic approach,we identified a small fraction of soft tumor cells in bladder cancer cells.More importantly,the existence of soft tumor cells was confirmed in clinical human bladder cancer specimens,in which the number of soft tumor cells was associated with tumor relapse.Furthermore,we demonstrated that the biomechanical stimuli arising from 3D Matrigel activated the F-actin/ITGB8/TRIM59/AKT/mTOR/glycolysis pathways to enhance the softness and tumorigenic capacity of tumor cells.Simultaneously,we detected a remarkable up-regulation in ITGB8,TRIM59,and phospho-AKT in clinical bladder recurrent tumors compared with their non-recurrent counterparts.Conclusions:The ITGB8/TRIM59/AKT/mTOR/glycolysis axis plays a crucial role in modulating tumor softness and stemness.Meanwhile,the soft tumor cells become more sensitive to chemotherapy after stiffening,that offers new insights for hampering tumor progression and recurrence.
