Anti-Mullerian Hormone (AMH) is a dimeric glycoprotein with a molecular weight of 140 kD, encoded by a gene on the short arm of chromosome and a member of the transforming growth factor-beta (TGF-<em>β</em&g...Anti-Mullerian Hormone (AMH) is a dimeric glycoprotein with a molecular weight of 140 kD, encoded by a gene on the short arm of chromosome and a member of the transforming growth factor-beta (TGF-<em>β</em>) superfamily. The expression of AMH is markedly different in males and females, both in concentration and temporality. In males, Sertoli cells maintain a high concentration of AMH in utero which peaks shortly after birth and then drops precipitously at puberty. In females, granulosa cells produce very low levels of AMH in utero followed by a transient spike in the neonatal period. Concentrations of the hormone then rise steadily through adolescence to a peak in the mid-twenties and subsequently decline until becoming undetectable in menopause. The study aimed to understand how Clinicians and Clinical Embryologists used anti-mullerian hormone (AMH) test to assess ovarian reserve, direct patient selection and treatment regimens and guide in vitro fertilization (IVF) cycle management in all registered fertility hospitals in a West African country, Ghana. A web-based survey (questionnaire) using google forms was performed to solicit responses from all IVF hospitals that are registered with the Fertility Society of Ghana (FERSOG). This questionnaire consisted of fifteen (15) broader questions, ten (10) of which assessed the clinics’ use of AMH. Responses were screened for quality to verify that only one (1) survey was completed by each IVF centre. The study was conducted during May and June 2020 at the In Vitro Fertilization (IVF) Department of the Airport Women’s Hospital (AWH) in Accra, Ghana. Results are reported as the proportion of IVF cycles represented by a particular answer choice. Survey responses were completed from 15 IVF centres, representing 2504 IVF cycles performed annually. A good majority (73.3%) [1835 IVF cycles] of the respondent IVF hospitals reported to use AMH as a first line test and 93.3% reported it as the best test for evaluating ovarian reserve. Another 66.7% reported that AMH results were extremely relevant to clinical practice. However, in contrast, for predicting live birth rate, 60% reported age as the best predictor in their practice. Overall, our results indicate that AMH is considered a first line test for assessing ovarian reserve and is relevant to the clinical practice of majority of Assisted Reproductive Technologies (ART) providers in Ghana.展开更多
With the increasing use of electronic gadgets, the effects of electromagnetic waves on sperm quality have been reported. This study evaluated the capacity of seminal antioxidants to protect spermatozoa from the effect...With the increasing use of electronic gadgets, the effects of electromagnetic waves on sperm quality have been reported. This study evaluated the capacity of seminal antioxidants to protect spermatozoa from the effects of exposure to non-ionizing cellular phone radiation on markers of semen quality and DNA integrity in an in-vitro model. A total of seventy-three (73) patients attending a fertility centre in Sakumono, Tema metropolis were recruited for this study. About 2 - 3 mls of freshly ejaculated semen was neatly collected by masturbation with consent and analysed according to the 2010 WHO standard semen analysis. A portion of Semen was exposed to non-ionizing cellular phone radiation for three (3) hours. Superoxide dismutase (SOD) activity levels and DNA fragmentation index (DFI) were determined before and after exposure. Data was entered and analysed using SPSS version 20.00 and Graph pad prism. DFI was positively associated with immobility (r = 0.233;p = 0.05) and negatively associated with percentage progressive motility (r = -0.258;p = 0.028), percentage total motility (r = -0.233;p = 0.05) and SOD levels (r = -0.270;p = 0.028). There was a significant positive linear relationship between SOD and progressive motility (r = 0.451;p < 0.0001), vitality (r = 0.725;p < 0.0001) and total motility (r = 0.705, p = 0.05). Higher seminal SOD level is protective against the damaging effects of electromagnetic waves on sperm quality.展开更多
文摘Anti-Mullerian Hormone (AMH) is a dimeric glycoprotein with a molecular weight of 140 kD, encoded by a gene on the short arm of chromosome and a member of the transforming growth factor-beta (TGF-<em>β</em>) superfamily. The expression of AMH is markedly different in males and females, both in concentration and temporality. In males, Sertoli cells maintain a high concentration of AMH in utero which peaks shortly after birth and then drops precipitously at puberty. In females, granulosa cells produce very low levels of AMH in utero followed by a transient spike in the neonatal period. Concentrations of the hormone then rise steadily through adolescence to a peak in the mid-twenties and subsequently decline until becoming undetectable in menopause. The study aimed to understand how Clinicians and Clinical Embryologists used anti-mullerian hormone (AMH) test to assess ovarian reserve, direct patient selection and treatment regimens and guide in vitro fertilization (IVF) cycle management in all registered fertility hospitals in a West African country, Ghana. A web-based survey (questionnaire) using google forms was performed to solicit responses from all IVF hospitals that are registered with the Fertility Society of Ghana (FERSOG). This questionnaire consisted of fifteen (15) broader questions, ten (10) of which assessed the clinics’ use of AMH. Responses were screened for quality to verify that only one (1) survey was completed by each IVF centre. The study was conducted during May and June 2020 at the In Vitro Fertilization (IVF) Department of the Airport Women’s Hospital (AWH) in Accra, Ghana. Results are reported as the proportion of IVF cycles represented by a particular answer choice. Survey responses were completed from 15 IVF centres, representing 2504 IVF cycles performed annually. A good majority (73.3%) [1835 IVF cycles] of the respondent IVF hospitals reported to use AMH as a first line test and 93.3% reported it as the best test for evaluating ovarian reserve. Another 66.7% reported that AMH results were extremely relevant to clinical practice. However, in contrast, for predicting live birth rate, 60% reported age as the best predictor in their practice. Overall, our results indicate that AMH is considered a first line test for assessing ovarian reserve and is relevant to the clinical practice of majority of Assisted Reproductive Technologies (ART) providers in Ghana.
文摘With the increasing use of electronic gadgets, the effects of electromagnetic waves on sperm quality have been reported. This study evaluated the capacity of seminal antioxidants to protect spermatozoa from the effects of exposure to non-ionizing cellular phone radiation on markers of semen quality and DNA integrity in an in-vitro model. A total of seventy-three (73) patients attending a fertility centre in Sakumono, Tema metropolis were recruited for this study. About 2 - 3 mls of freshly ejaculated semen was neatly collected by masturbation with consent and analysed according to the 2010 WHO standard semen analysis. A portion of Semen was exposed to non-ionizing cellular phone radiation for three (3) hours. Superoxide dismutase (SOD) activity levels and DNA fragmentation index (DFI) were determined before and after exposure. Data was entered and analysed using SPSS version 20.00 and Graph pad prism. DFI was positively associated with immobility (r = 0.233;p = 0.05) and negatively associated with percentage progressive motility (r = -0.258;p = 0.028), percentage total motility (r = -0.233;p = 0.05) and SOD levels (r = -0.270;p = 0.028). There was a significant positive linear relationship between SOD and progressive motility (r = 0.451;p < 0.0001), vitality (r = 0.725;p < 0.0001) and total motility (r = 0.705, p = 0.05). Higher seminal SOD level is protective against the damaging effects of electromagnetic waves on sperm quality.
