Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the analysis of the topology of membrane protein. Agrobacterium strain A6007 was mutagenized with E. coli strain mm294A plasmid pRK...Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the analysis of the topology of membrane protein. Agrobacterium strain A6007 was mutagenized with E. coli strain mm294A plasmid pRK609 having TnphoA to obtain mutants defective in virulence. Because alkaline phosphatase activity is only detected when the PhoA gene product from the transposon is secreted out of the protoplasm, the virulence mutants are located in genes that code for transmembrane or periplasmic proteins. Attempts were made to obtain the sequences adjacent to the TnphoA inserts through several different approaches including Inverse PCR, Cloning, and Tail PCR. Transposon-adjacent sequence was obtained from one membrane anchor subunit in Bradyrhizobium japonicum i.e. succinate dehydrogenase which has enhanced transformation efficiency.展开更多
As China is emerging as an economic super power of global proportion, there is merit incomparing it to the performance of the Indian economy, particularly during its post reformera. In this article the author focuses ...As China is emerging as an economic super power of global proportion, there is merit incomparing it to the performance of the Indian economy, particularly during its post reformera. In this article the author focuses on the two economies and analyses why China turnedout to be commendable economic success while India, despite having minor achievements,cannot be considered a comparable case. Although China started on its reform path from asomewhat lower economic level, it outperformed India by a wide margin. In all the measurableareas of economic performance, China measures far superior to India. The article makes aclose comparison and explains the reasons behind the lackluster performance of the Indiaeconomy. Delayed, poorly visualized, shallow and inadequately implemented reforms isonly one of the myriad of reasons behind Indian economy’s jaded and uninspiring show.There are several traditional non-economic idiosyncrasies that caused the Indian economyto under-perform.展开更多
文摘Protein fusion with the Escherichia coli alkaline phosphatase is used extensively for the analysis of the topology of membrane protein. Agrobacterium strain A6007 was mutagenized with E. coli strain mm294A plasmid pRK609 having TnphoA to obtain mutants defective in virulence. Because alkaline phosphatase activity is only detected when the PhoA gene product from the transposon is secreted out of the protoplasm, the virulence mutants are located in genes that code for transmembrane or periplasmic proteins. Attempts were made to obtain the sequences adjacent to the TnphoA inserts through several different approaches including Inverse PCR, Cloning, and Tail PCR. Transposon-adjacent sequence was obtained from one membrane anchor subunit in Bradyrhizobium japonicum i.e. succinate dehydrogenase which has enhanced transformation efficiency.
文摘As China is emerging as an economic super power of global proportion, there is merit incomparing it to the performance of the Indian economy, particularly during its post reformera. In this article the author focuses on the two economies and analyses why China turnedout to be commendable economic success while India, despite having minor achievements,cannot be considered a comparable case. Although China started on its reform path from asomewhat lower economic level, it outperformed India by a wide margin. In all the measurableareas of economic performance, China measures far superior to India. The article makes aclose comparison and explains the reasons behind the lackluster performance of the Indiaeconomy. Delayed, poorly visualized, shallow and inadequately implemented reforms isonly one of the myriad of reasons behind Indian economy’s jaded and uninspiring show.There are several traditional non-economic idiosyncrasies that caused the Indian economyto under-perform.
