Studies have shown that phosphatase and tensin homolog deleted on chromosome ten(PTEN)participates in the regulation of cochlear hair cell survival.Bisperoxovanadium protects against neurodegeneration by inhibiting PT...Studies have shown that phosphatase and tensin homolog deleted on chromosome ten(PTEN)participates in the regulation of cochlear hair cell survival.Bisperoxovanadium protects against neurodegeneration by inhibiting PTEN expression.However,whether bisperoxovanadium can protect against noise-induced hearing loss and the underlying mechanism remains unclear.In this study,we established a mouse model of noise-induced hearing loss by exposure to 105 dB sound for 2 hours.We found that PTEN expression was increased in the organ of Corti,including outer hair cells,inner hair cells,and lateral wall tissues.Intraperitoneal administration of bisperoxovanadium decreased the auditory threshold and the loss of cochlear hair cells and inner hair cell ribbons.In addition,noise exposure decreased p-PI3K and p-Akt levels.Bisperoxovanadium preconditioning or PTEN knockdown upregulated the activity of PI3K-Akt.Bisperoxovanadium also prevented H_(2)O_(2)-induced hair cell death by reducing mitochondrial reactive oxygen species generation in cochlear explants.These findings suggest that bisperoxovanadium reduces noise-induced hearing injury and reduces cochlear hair cell loss.展开更多
Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss.In order to elucidate the mechanism underlying these disorders,OHCs-its dis...Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss.In order to elucidate the mechanism underlying these disorders,OHCs-its diseased region needs to be deeply investigated.However,OHCs array on the basilar membrane which contains massive cells with different types.Therefore,to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing.In the present study,we tried to isolate OHCs from the commonly used animal model-Sprague-Dawley (SD) rats.By separating outer hair cells from SD rats with different day ages,we found that 9 days after birth was a suitable time for the separation of the OHCs.At this time,the number of OHCs isolated from rats was large,and the cell morphology was typical of cylindrical shape.OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses.展开更多
基金supported by the National Natural Science Foundation of China,Nos.81670925(to FQC),81870732(to DJZ),81800918(to WL),81900933(to YLS)Department of Science and Technology Key Industry Innovation Chain Social Development Field Fund of Shaanxi Province,No.2021ZDLSF02-12(to FQC)the Natural Science Foundation of Shaanxi Province,No.2019JM-009(to JC).
文摘Studies have shown that phosphatase and tensin homolog deleted on chromosome ten(PTEN)participates in the regulation of cochlear hair cell survival.Bisperoxovanadium protects against neurodegeneration by inhibiting PTEN expression.However,whether bisperoxovanadium can protect against noise-induced hearing loss and the underlying mechanism remains unclear.In this study,we established a mouse model of noise-induced hearing loss by exposure to 105 dB sound for 2 hours.We found that PTEN expression was increased in the organ of Corti,including outer hair cells,inner hair cells,and lateral wall tissues.Intraperitoneal administration of bisperoxovanadium decreased the auditory threshold and the loss of cochlear hair cells and inner hair cell ribbons.In addition,noise exposure decreased p-PI3K and p-Akt levels.Bisperoxovanadium preconditioning or PTEN knockdown upregulated the activity of PI3K-Akt.Bisperoxovanadium also prevented H_(2)O_(2)-induced hair cell death by reducing mitochondrial reactive oxygen species generation in cochlear explants.These findings suggest that bisperoxovanadium reduces noise-induced hearing injury and reduces cochlear hair cell loss.
基金the National Natural Science Foundation of China(NSFC 81120108008).
文摘Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss.In order to elucidate the mechanism underlying these disorders,OHCs-its diseased region needs to be deeply investigated.However,OHCs array on the basilar membrane which contains massive cells with different types.Therefore,to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing.In the present study,we tried to isolate OHCs from the commonly used animal model-Sprague-Dawley (SD) rats.By separating outer hair cells from SD rats with different day ages,we found that 9 days after birth was a suitable time for the separation of the OHCs.At this time,the number of OHCs isolated from rats was large,and the cell morphology was typical of cylindrical shape.OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses.
