Modelling and Dynamic Characteristics for a Non-metal Pressurized Reservoir with Variable Volume

With the increasing demand to reduce emissions and save energy,hydraulic reservoirs require new architecture to optimize their weight,space,and volume.Conventional open reservoirs are large,heavy,and easily polluted,and threaten the operation of hydraulic systems.A closed reservoir provides the advantages of small volume and light weight,compared to open reservoirs.In this study,a non-metallic pressure reservoir with variable volume is designed and manufactured for closed-circuit hydraulic systems.The reservoir housing is made of rubber,and the Mooney-Rivlin model is chosen based on the rubber strain properties.The FEA simulation for the reservoir is performed using ANSYS Workbench to obtain the structural stiffness.The major contribution is the establishment of mathematical models for this reservoir,including the volume equation changing with height,flow equation,and force balance equation,to explore the output characteristics of this reservoir.Based on these results,simulation models were built to analyze the output characteristics of the reservoir.Moreover,the test rig of a conventional hydraulic system was transformed into a closed-circuit asymmetric hydraulic system for the reservoir,and preliminary verification experiments were conducted on it.The results demonstrate that the designed reservoir can absorb and discharge oil and supercharge pump inlet to benefit system operation.The changes in the volume and pressure with displacements under different volume ratios and frequencies were obtained,which verified the accuracy of the mathematical models.Owing to its lightweight design and small volume,the reservoir can replace conventional open reservoirs,and this lays a foundation for future theoretical research on this reservoir.

GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity

Gasdermin B (GSDMB) has been reported to be associated with immune diseases in humans, but the detailed molecular mechanisms remain unsolved. The N-terminus of GSDMB by itself, unlike other gasdermin family proteins, does not induce cell death. Here, we show that GSDMB is highly expressed in the leukocytes of septic shock patients, which is associated with increased release of the gasdermin D (GSDMD) N-terminus. GSDMB expression and the accumulation of the N-terminal fragment of GSDMD are induced by the activation of the non-canonical pyroptosis pathway in a human monocyte cell line. The downregulation of GSDMB alleviates the cleavage of GSDMD and cell death. Consistently, the overexpression of GSDMB promotes GSDMD cleavage, accompanied by increased LDH release. We further found that GSDMB promotes caspase-4 activity, which is required for the cleavage of GSDMD in non-canonical pyroptosis, by directly binding to the CARD domain of caspase-4. Our study reveals a GSDMB-mediated novel regulatory mechanism for non-canonical pyroptosis and suggests a potential new strategy for the treatment of inflammatory diseases.