A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfuse...A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture.展开更多
文摘A Super\|Spinner was Modified by mounting a stainless steel filter(pore size 75 μm)to the impeller shaft to retain cells while fresh nutrient is perfused.Using Macroporous microcarrier Cytopore 1,continuously perfused cultivation of a recombinant CHO cell line,CHO2DS producing prothrombin was performed with the perfusion of a protein\|free medium DF6S.The cell retention rate was more than 90% during the 24 days continuously perfused cultivation.The viable cell density of CHO2DS and prothrombin concentration reached 4.62×10 6(cells.m/L) and 11.3(mg/L)respectively after 9 days culture.
