Clinical use of corticosteroid （CS） therapy and links with adverse neurological effects CS therapy is widely used in clinical practice worldwide, with ad- ministration of high and multiple doses prescribed for a ran...Clinical use of corticosteroid （CS） therapy and links with adverse neurological effects CS therapy is widely used in clinical practice worldwide, with ad- ministration of high and multiple doses prescribed for a range of disease and injury. Notably, CS have been used since the 1950＇s for the treatment of suspected respiratory distress syndrome, in order to accelerate lung maturation in premature babies, as also in antenatal therapy to pregnant women at risk of preterm birth （Haddad et al., 1956; Shinwell and Eventov-Friedman, 2009; Bonanno and Wapner, 2012）. High dose immunosuppressive CS therapy is also widely used in the treatment of multiple sclerosis （MS） and spinal cord injury （Bracken, 2012; Burton et al., 2012）. However,展开更多
The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavil...The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily reliant on viral transduction methods, which have safety and production limitations. Magnetofection （magnet-assisted gene transfer using iron oxide nanoparticles as vectors） has emerged as a highly promising non-viral alternative for safe and reproducible genetic modification of neurons. Despite the high potential of this technology, there is an important gap in our knowledge of the safety of this approach, namely, whether it alters neuronal function in adverse ways, such as by altering neuronal excitability and signaling. We have investigated the effects of magnetofection in primary cortical neurons by examining neuronal excitability using the whole cell patch clamp technique. We found no evidence that magnetofection alters the voltage-dependent sodium and potassium ionic currents that underpin excitability. Our study provides important new data supporting magnetofection as a safe technology for bioengineering of neuronal cell populations.展开更多
基金funded through grants from the British Neuropathological Society,North Staffordshire Medical Institute and University of Nottingham
文摘Clinical use of corticosteroid （CS） therapy and links with adverse neurological effects CS therapy is widely used in clinical practice worldwide, with ad- ministration of high and multiple doses prescribed for a range of disease and injury. Notably, CS have been used since the 1950＇s for the treatment of suspected respiratory distress syndrome, in order to accelerate lung maturation in premature babies, as also in antenatal therapy to pregnant women at risk of preterm birth （Haddad et al., 1956; Shinwell and Eventov-Friedman, 2009; Bonanno and Wapner, 2012）. High dose immunosuppressive CS therapy is also widely used in the treatment of multiple sclerosis （MS） and spinal cord injury （Bracken, 2012; Burton et al., 2012）. However,
文摘The development of safe technologies to genetically modify neurons is of great interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily reliant on viral transduction methods, which have safety and production limitations. Magnetofection （magnet-assisted gene transfer using iron oxide nanoparticles as vectors） has emerged as a highly promising non-viral alternative for safe and reproducible genetic modification of neurons. Despite the high potential of this technology, there is an important gap in our knowledge of the safety of this approach, namely, whether it alters neuronal function in adverse ways, such as by altering neuronal excitability and signaling. We have investigated the effects of magnetofection in primary cortical neurons by examining neuronal excitability using the whole cell patch clamp technique. We found no evidence that magnetofection alters the voltage-dependent sodium and potassium ionic currents that underpin excitability. Our study provides important new data supporting magnetofection as a safe technology for bioengineering of neuronal cell populations.
