A rice CaMBP gene, OsCaMBP (AB363406), was isolated from a chilling treated rice using the fluorescent differential display (FDD) screening method. Its cDNA sequence (2094 bp) contains an opening reading frame ...A rice CaMBP gene, OsCaMBP (AB363406), was isolated from a chilling treated rice using the fluorescent differential display (FDD) screening method. Its cDNA sequence (2094 bp) contains an opening reading frame (ORF) encoding a 569 amino acids protein (63.2 kD). OsCaMBP has the typical structural features of the CaMBP family, including the conserved IQ calmodulin-binding motif at the N-terminus. Homology analysis revealed 38.25%-47.28% identities of OsCaMBP with other CaMBPs in plants. RT-PCR analysis showed that the expression of OsCaMBP was remarkably inducible under the chilling (8℃) and heat-shock (42℃) treatments. OsCaMBP was undetectable under the normal conditions, and induced under the chilling treatment for 1 h, as well as the heat-shock treatment for 15 min, suggesting that the gene plays important roles in the signaling pathway in rice under both chilling and heat-shock stresses.展开更多
基金supported by the Program for Changjiang Scholars and Innovative Research Team in University of China (Grant No. IRT0453)the National Science & Technology Pillar Program of China in the Eleventh Five-Year Plan Period (Grant No. 2007BAD81B00).
文摘A rice CaMBP gene, OsCaMBP (AB363406), was isolated from a chilling treated rice using the fluorescent differential display (FDD) screening method. Its cDNA sequence (2094 bp) contains an opening reading frame (ORF) encoding a 569 amino acids protein (63.2 kD). OsCaMBP has the typical structural features of the CaMBP family, including the conserved IQ calmodulin-binding motif at the N-terminus. Homology analysis revealed 38.25%-47.28% identities of OsCaMBP with other CaMBPs in plants. RT-PCR analysis showed that the expression of OsCaMBP was remarkably inducible under the chilling (8℃) and heat-shock (42℃) treatments. OsCaMBP was undetectable under the normal conditions, and induced under the chilling treatment for 1 h, as well as the heat-shock treatment for 15 min, suggesting that the gene plays important roles in the signaling pathway in rice under both chilling and heat-shock stresses.