Simultaneous localization and mapping(SLAM)is one of the most attractive research hotspots in the field of robotics,and it is also a prerequisite for the autonomous navigation of robots.It can significantly improve th...Simultaneous localization and mapping(SLAM)is one of the most attractive research hotspots in the field of robotics,and it is also a prerequisite for the autonomous navigation of robots.It can significantly improve the autonomous navigation ability of mobile robots and their adaptability to different application environments and contribute to the realization of real-time obstacle avoidance and dynamic path planning.Moreover,the application of SLAM technology has expanded from industrial production,intelligent transportation,special operations and other fields to agricultural environments,such as autonomous navigation,independent weeding,three-dimen-sional(3D)mapping,and independent harvesting.This paper mainly introduces the principle,sys-tem framework,latest development and application of SLAM technology,especially in agricultural environments.Firstly,the system framework and theory of the SLAM algorithm are introduced,and the SLAM algorithm is described in detail according to different sensor types.Then,the devel-opment and application of SLAM in the agricultural environment are summarized from two aspects:environment map construction,and localization and navigation of agricultural robots.Finally,the challenges and future research directions of SLAM in the agricultural environment are discussed.展开更多
In the process of aquaculture,monitoring the number of fish bait particles is of great significance to improve the growth and welfare of fish.Although the counting method based on onvolutional neural network(CNN)achie...In the process of aquaculture,monitoring the number of fish bait particles is of great significance to improve the growth and welfare of fish.Although the counting method based on onvolutional neural network(CNN)achieve good accuracy and applicability,it has a high amount of parameters and computation,which limit the deployment on resource-constrained hardware devices.In order to solve the above problems,this paper proposes a lightweight bait particle counting method based on shift quantization and model pruning strategies.Firstly,we take corresponding lightweight strategies for different layers to flexibly balance the counting accuracy and performance of the model.In order to deeply lighten the counting model,the redundant and less informative weights of the model are removed through the combination of model quantization and pruning.The experimental results show that the compression rate is nearly 9 times.Finally,the quantization candidate value is refined by introducing a power-of-two addition term,which improves the matches of the weight distribution.By analyzing the experimental results,the counting loss at 3 bit is reduced by 35.31%.In summary,the lightweight bait particle counting model proposed in this paper achieves lossless counting accuracy and reduces the storage and computational overhead required for running convolutional neural networks.展开更多
Facility agriculture is an essential carrier for promoting stable production and supply.In2020,the planting area of facility agriculture in Beijing(290098000 m2)accounted for 29.06%of the total planting area of crops(...Facility agriculture is an essential carrier for promoting stable production and supply.In2020,the planting area of facility agriculture in Beijing(290098000 m2)accounted for 29.06%of the total planting area of crops(998078000 m2),and the output value accounted for 46.56%.In2022,Beijing Agricultural Technology Extension Station organized relevant departments to summarize and evaluate the agricultural facilities in Beijing through consultation materials,interviews,questionnaires,and field trips,combining survey data and statistical data to draw the following conclusions.Facilities play a major supporting role in stabilizing production and ensuring supply.The number of traditional greenhouses is extensive and covers a large area,so special planning and classification upgrades are urgently needed.Facilities are rich in scientific and technological resources,but the mode of production is out of date.It is of great significance to improve the comprehensive production capacity of facility agriculture and promote the healthy and stable development of facility industry in Beijing to increase the utilization rate of facility agriculture land,strengthen the support of facility science and technology,and cultivate the socialized service organization of facility.展开更多
时序分析是合成孔径雷达干涉测量(InSAR)的一项关键技术,它被广泛应用于监测广阔地区的地表缓慢形变。这种方法能够提供大范围、大面积的形变监测。其中,StaMPS PS方法凭借适用范围广、开源等优点,受到众多学者的使用。但在时序InSAR中...时序分析是合成孔径雷达干涉测量(InSAR)的一项关键技术,它被广泛应用于监测广阔地区的地表缓慢形变。这种方法能够提供大范围、大面积的形变监测。其中,StaMPS PS方法凭借适用范围广、开源等优点,受到众多学者的使用。但在时序InSAR中,对流层延迟相位会导致形变监测精度的降低,因此,以合肥市为研究区域,分析了经验模型线性改正、GACOS(generic atmospheric correction online service for InSAR)改正和欧洲中期天气预报中心(ECMWF)最新发布的ERA5数据集改正,并对比这3种方法在时序InSAR反演形变速率中的改正效果。通过计算得到研究区的标准差,进行比较分析和验证。其中线性改正、ERA5和GACOS改正后的标准差分别降低了21.71%、16.14%、10.38%。对于合肥区域,这3种方法均可减弱对流层延迟的影响,且精度都有所提高,其中线性改正效果最好,适用性更高,ERA5和GACOS受天气以及地面监测点密度等影响,在该区域改正效果较差。展开更多
Coxsackievirus A16(CVA16),together with enterovirus type 71(EV71),is responsible for most cases of hand,foot and mouth disease(HFMD) worldwide.Recent findings suggest that the recombination between CVA16 and EV71,and ...Coxsackievirus A16(CVA16),together with enterovirus type 71(EV71),is responsible for most cases of hand,foot and mouth disease(HFMD) worldwide.Recent findings suggest that the recombination between CVA16 and EV71,and the co-circulation of these two viruses may have contributed to the increase of HFMD cases in China over the past few years.It is therefore important to further understand the virology,epidemiology,virus-host interactions and host pathogenesis of CVA16.In this study,we describe the viral kinetics of CVA16 in human rhabdomyosarcoma(RD) cells by analyzing the cytopathic effect(CPE),viral RNA replication,viral protein expression,viral RNA package and viral particle secretion in RD cells.We show that CVA16 appears to first attach,uncoat and enter into the host cell after adsorption for 1 h.Later on,CVA16 undergoes rapid replication from 3 to 6 h at MOI 1 and until 9 h at MOI 0.1.At MOI 0.1,CVA16 initiates a secondary infection as the virions were secreted before 9 h p.i.CPE was observed after 12 h p.i.,and viral antigen was first detected at 6 h p.i.at MOI 1 and at 9 h p.i.at MOI 0.1.Thus,our study provides important information for further investigation of CVA16 in order to better understand and ultimately control infections with this virus.展开更多
Thunniform swimmers(tuna)have a swinging narrow sequence stalk and a moon-shaped tail fin,which performs poorly at slow speed,higher acceleration and turning maneuverability.In most cases,faster speed and higher maneu...Thunniform swimmers(tuna)have a swinging narrow sequence stalk and a moon-shaped tail fin,which performs poorly at slow speed,higher acceleration and turning maneuverability.In most cases,faster speed and higher maneuverability are mutually rejection for most marine creatures and their robotic opponents.This paper presents a novel hybrid tuna-like swimming robot for aquaculture water quality monitoring,which interleaves faster speed and higher maneuverability.The robotic prototype emphasizes on streamlining and enhanced maneuverability mechanism designs in conjunction with a narrow caudal propeller to the tail.The innovative design endows the robot to easily execute the multi-mode swimming gait,including forward swimming,turning,diving/surfacing.The capabilities of our robot are validated through a series of indoor swimming pool and field breeding ponds.The robotic fish can achieve a maximum speed up to about 1.16 m/s and a minimum turning radius less than 0.46 Body Lengths(BL)and its maximum turning speed can reach 78.6∘/s.Due to its high speed,maneuverability and relatively small size,the robotic fish shed light on intelligent monitoring in complex aquatic environments.展开更多
Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenes...Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenesis of neural tube defects,we explored spatiotemporal expression of LIFR at different stages of fetal development in normal and neural tube defect embryos.Spina bifida aperta was induced with all-trans retinoic acid on embryonic day 10 in rats,and the spatiotemporal expression of LIFR was investigated in spina bifida aperta rats and healthy rats from embryonic day 11 to 17.Real time-polymerase chain reaction and western blot assay were used to examine mRNA and protein expression of LIFR in healthy control and neural tube defect embryos.Results of the animal experiment demonstrated that expression of LIFR protein and mRNA in the spinal cords of normal rat embryos increased with embryonic development.LIFR was significantly downregulated in the spinal cords of spina bifida aperta rats compared with healthy rats from embryonic days 11 to 17.Immunohistochemical staining showed that the expression of LIFR in placenta and spinal cord in spina bifida aperta rat embryos was decreased compared with that in control embryos at embryonic day 15.Results from human embryo specimens showed that LIFR mRNA expression was significantly down-regulated in spinal cords of human fetuses with neural tube defects compared with normal controls at a gestational age of 24 to 33 weeks.The results were consistent with the down-regulation of LIFR in the animal experiments.Our study revealed spatiotemporal changes in expression of LIFR during embryonic neurulation.Thus,LIFR might play a specific role in neural tube development.All animal and human experimental procedures were approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS106K)on February 25,2016.展开更多
This paper develops a real-time PV arrays maximum power harvesting scheme under partial shading condition(PSC)by reconfiguring PV arrays using Aquila optimizer(AO).AO is based on the natural behaviors of Aquila in cap...This paper develops a real-time PV arrays maximum power harvesting scheme under partial shading condition(PSC)by reconfiguring PV arrays using Aquila optimizer(AO).AO is based on the natural behaviors of Aquila in capturing prey,which can choose the best hunting mechanism ingeniously and quickly by balancing the local exploitation and global exploration via four hunting methods of Aquila:choosing the searching area through high soar with the vertical stoop,exploring in different searching spaces through contour flight with quick glide attack,exploiting in convergence searching space through low flight with slow attack,and swooping through walk and grabbing prey.In general,PV arrays reconfiguration is a problem of discrete optimization,thus a series of discrete operations are adopted in AO to enhance its optimization performance.Simulation results based on 10 cases under PSCs show that the mismatched power loss obtained by AO is the smallest compared with genetic algorithm,particle swarm optimization,ant colony algorithm,grasshopper optimization algorithm,and butterfly optimization algorithm,which reduced by 4.34%against butterfly optimization algorithm.展开更多
Efficient evaluation of crop phenotypes is a prerequisite for breeding, cultivar adoption, genomics and phenomics study. Plant genotyping is developing rapidly through the use of high-throughput sequencing techniques,...Efficient evaluation of crop phenotypes is a prerequisite for breeding, cultivar adoption, genomics and phenomics study. Plant genotyping is developing rapidly through the use of high-throughput sequencing techniques,while plant phenotyping has lagged far behind and it has become the rate-limiting factor in genetics, large-scale breeding and development of new cultivars. In this paper,we consider crop phenotyping technology under three categories. The first is high-throughput phenotyping techniques in controlled environments such as greenhouses or specifically designed platforms. The second is a phenotypic strengthening test in semi-controlled environments, especially for traits that are difficult to be tested in multi-environment trials(MET), such as lodging, drought and disease resistance. The third is MET in uncontrolled environments, in which crop plants are managed according to farmer's cultural practices. Research and application of these phenotyping techniques are reviewed and methods for MET improvement proposed.展开更多
False seeds can often be seen in the maize seed market,leading to a serious decline in maize yield.Those existing variety identification methods are expensive,time consuming,and destructive to seeds.The aim of this st...False seeds can often be seen in the maize seed market,leading to a serious decline in maize yield.Those existing variety identification methods are expensive,time consuming,and destructive to seeds.The aim of this study is to develop a cheap,fast and non-destructive method which can robustly identify large amounts of maize seed varieties based on near-infrared reflectance spectroscopy(NIRS)and chemometrics.Because it is difficult to establish models for every variety in the market,this study mainly investigated the performance of models based on a large number of samples(more than 40 major varieties in the market).The reflectance spectra of maize seeds were collected by two modes(bulk kernels mode and single kernel mode).Both collection modes can be applied to identification,but only the single kernel mode can be applied to purity sorting.The spectra were pretreated with smoothing,the first derivative and vector normalization;and then principal component analysis(PCA),linear discriminant analysis(LDA)and biomimetic pattern recognition(BPR)were applied to establish identification models.The environmental factors such as producing areas and years have a significant influence on the performance of the models.Therefore,the method to improve the robustness of the models was investigated in this study.New indexes(correct acceptance degree(CAD),correct rejection degree(CRD)and correct degree(CD))were defined to analyze the performance of the models more accurately.Finally,the models obtained a mean correct discrimination rate of over 90%,and exhibited robust properties for samples harvested from different areas and years.The results showed that NIR technology combined with chemometrics methods such as PCA,LDA,and BPR could be a suitable and alternative technique to identify the authenticity of maize seed varieties.展开更多
Transposable elements(TEs)constitute the most abundant portions of plant genomes and can dramatically shape host genomes during plant evolution.They also play important roles in crop domestication.However,whether TEs ...Transposable elements(TEs)constitute the most abundant portions of plant genomes and can dramatically shape host genomes during plant evolution.They also play important roles in crop domestication.However,whether TEs themselves are also selected during crop domestication has remained unknown.Here,we identify an active long terminal repeat(LTR)retrotransposon,HUO,as a potential target of selection during rice domestication and breeding.HUO is a low-copy-number LTR retrotransposon,and is active under natural growth conditions and transmitted through male gametogenesis,preferentially inserting into genomic regions capable of transcription.HUO exists in all wild rice accessions and about half of the archaeological rice grains(1200–7000 years ago)and landraces surveyed,but is absent in almost all modern varieties,indicating its gradual elimination during rice domestication and breeding.Further analyses showed that HUO is subjected to strict gene silencing through the RNA-directed DNA methylation pathway.Our results also suggest that multiple HUO copies may trigger genomic instability through altering genome-wide DNA methylation and small RNA biogenesis and changing global gene expression,resulting in decreased disease resistance and yield,coinciding with its elimination during rice breeding.Together,our study suggests that negative selection of an active retrotransposon might be important for genome stability during crop domestication and breeding.展开更多
基金supported by the National Key Research and Development Program(No.2022YFD2001704).
文摘Simultaneous localization and mapping(SLAM)is one of the most attractive research hotspots in the field of robotics,and it is also a prerequisite for the autonomous navigation of robots.It can significantly improve the autonomous navigation ability of mobile robots and their adaptability to different application environments and contribute to the realization of real-time obstacle avoidance and dynamic path planning.Moreover,the application of SLAM technology has expanded from industrial production,intelligent transportation,special operations and other fields to agricultural environments,such as autonomous navigation,independent weeding,three-dimen-sional(3D)mapping,and independent harvesting.This paper mainly introduces the principle,sys-tem framework,latest development and application of SLAM technology,especially in agricultural environments.Firstly,the system framework and theory of the SLAM algorithm are introduced,and the SLAM algorithm is described in detail according to different sensor types.Then,the devel-opment and application of SLAM in the agricultural environment are summarized from two aspects:environment map construction,and localization and navigation of agricultural robots.Finally,the challenges and future research directions of SLAM in the agricultural environment are discussed.
基金supported by the National Key Research and Development Program of China(No.2019YFD0901000)。
文摘In the process of aquaculture,monitoring the number of fish bait particles is of great significance to improve the growth and welfare of fish.Although the counting method based on onvolutional neural network(CNN)achieve good accuracy and applicability,it has a high amount of parameters and computation,which limit the deployment on resource-constrained hardware devices.In order to solve the above problems,this paper proposes a lightweight bait particle counting method based on shift quantization and model pruning strategies.Firstly,we take corresponding lightweight strategies for different layers to flexibly balance the counting accuracy and performance of the model.In order to deeply lighten the counting model,the redundant and less informative weights of the model are removed through the combination of model quantization and pruning.The experimental results show that the compression rate is nearly 9 times.Finally,the quantization candidate value is refined by introducing a power-of-two addition term,which improves the matches of the weight distribution.By analyzing the experimental results,the counting loss at 3 bit is reduced by 35.31%.In summary,the lightweight bait particle counting model proposed in this paper achieves lossless counting accuracy and reduces the storage and computational overhead required for running convolutional neural networks.
基金supported by the Rural Revitalization Science and Technology Project of Beijing(No.20221230-01)Beijing Agriculture Innovation Consortium(No.BAIC 2022-1)。
文摘Facility agriculture is an essential carrier for promoting stable production and supply.In2020,the planting area of facility agriculture in Beijing(290098000 m2)accounted for 29.06%of the total planting area of crops(998078000 m2),and the output value accounted for 46.56%.In2022,Beijing Agricultural Technology Extension Station organized relevant departments to summarize and evaluate the agricultural facilities in Beijing through consultation materials,interviews,questionnaires,and field trips,combining survey data and statistical data to draw the following conclusions.Facilities play a major supporting role in stabilizing production and ensuring supply.The number of traditional greenhouses is extensive and covers a large area,so special planning and classification upgrades are urgently needed.Facilities are rich in scientific and technological resources,but the mode of production is out of date.It is of great significance to improve the comprehensive production capacity of facility agriculture and promote the healthy and stable development of facility industry in Beijing to increase the utilization rate of facility agriculture land,strengthen the support of facility science and technology,and cultivate the socialized service organization of facility.
文摘时序分析是合成孔径雷达干涉测量(InSAR)的一项关键技术,它被广泛应用于监测广阔地区的地表缓慢形变。这种方法能够提供大范围、大面积的形变监测。其中,StaMPS PS方法凭借适用范围广、开源等优点,受到众多学者的使用。但在时序InSAR中,对流层延迟相位会导致形变监测精度的降低,因此,以合肥市为研究区域,分析了经验模型线性改正、GACOS(generic atmospheric correction online service for InSAR)改正和欧洲中期天气预报中心(ECMWF)最新发布的ERA5数据集改正,并对比这3种方法在时序InSAR反演形变速率中的改正效果。通过计算得到研究区的标准差,进行比较分析和验证。其中线性改正、ERA5和GACOS改正后的标准差分别降低了21.71%、16.14%、10.38%。对于合肥区域,这3种方法均可减弱对流层延迟的影响,且精度都有所提高,其中线性改正效果最好,适用性更高,ERA5和GACOS受天气以及地面监测点密度等影响,在该区域改正效果较差。
基金Partly supported by the National Natural Science Foundation of China (No. 20872048)
文摘Coxsackievirus A16(CVA16),together with enterovirus type 71(EV71),is responsible for most cases of hand,foot and mouth disease(HFMD) worldwide.Recent findings suggest that the recombination between CVA16 and EV71,and the co-circulation of these two viruses may have contributed to the increase of HFMD cases in China over the past few years.It is therefore important to further understand the virology,epidemiology,virus-host interactions and host pathogenesis of CVA16.In this study,we describe the viral kinetics of CVA16 in human rhabdomyosarcoma(RD) cells by analyzing the cytopathic effect(CPE),viral RNA replication,viral protein expression,viral RNA package and viral particle secretion in RD cells.We show that CVA16 appears to first attach,uncoat and enter into the host cell after adsorption for 1 h.Later on,CVA16 undergoes rapid replication from 3 to 6 h at MOI 1 and until 9 h at MOI 0.1.At MOI 0.1,CVA16 initiates a secondary infection as the virions were secreted before 9 h p.i.CPE was observed after 12 h p.i.,and viral antigen was first detected at 6 h p.i.at MOI 1 and at 9 h p.i.at MOI 0.1.Thus,our study provides important information for further investigation of CVA16 in order to better understand and ultimately control infections with this virus.
基金the National Key R&D Program of China(2022YFE0107100)the National Key R&D Programs of China(Grant No.2019YFD0901000)the National Natural Science Foundation of China(Grant No.61903007).
文摘Thunniform swimmers(tuna)have a swinging narrow sequence stalk and a moon-shaped tail fin,which performs poorly at slow speed,higher acceleration and turning maneuverability.In most cases,faster speed and higher maneuverability are mutually rejection for most marine creatures and their robotic opponents.This paper presents a novel hybrid tuna-like swimming robot for aquaculture water quality monitoring,which interleaves faster speed and higher maneuverability.The robotic prototype emphasizes on streamlining and enhanced maneuverability mechanism designs in conjunction with a narrow caudal propeller to the tail.The innovative design endows the robot to easily execute the multi-mode swimming gait,including forward swimming,turning,diving/surfacing.The capabilities of our robot are validated through a series of indoor swimming pool and field breeding ponds.The robotic fish can achieve a maximum speed up to about 1.16 m/s and a minimum turning radius less than 0.46 Body Lengths(BL)and its maximum turning speed can reach 78.6∘/s.Due to its high speed,maneuverability and relatively small size,the robotic fish shed light on intelligent monitoring in complex aquatic environments.
基金supported by the National Natural Science Foundation of China,No.81601292(to DA),No.81671469(to ZWY)the National Basic Research Program of China(973 Program),No.2013CB945402(to ZWY)the National Key Research and Development Program of China,No.2016YFC1000505(to ZWY)
文摘Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenesis of neural tube defects,we explored spatiotemporal expression of LIFR at different stages of fetal development in normal and neural tube defect embryos.Spina bifida aperta was induced with all-trans retinoic acid on embryonic day 10 in rats,and the spatiotemporal expression of LIFR was investigated in spina bifida aperta rats and healthy rats from embryonic day 11 to 17.Real time-polymerase chain reaction and western blot assay were used to examine mRNA and protein expression of LIFR in healthy control and neural tube defect embryos.Results of the animal experiment demonstrated that expression of LIFR protein and mRNA in the spinal cords of normal rat embryos increased with embryonic development.LIFR was significantly downregulated in the spinal cords of spina bifida aperta rats compared with healthy rats from embryonic days 11 to 17.Immunohistochemical staining showed that the expression of LIFR in placenta and spinal cord in spina bifida aperta rat embryos was decreased compared with that in control embryos at embryonic day 15.Results from human embryo specimens showed that LIFR mRNA expression was significantly down-regulated in spinal cords of human fetuses with neural tube defects compared with normal controls at a gestational age of 24 to 33 weeks.The results were consistent with the down-regulation of LIFR in the animal experiments.Our study revealed spatiotemporal changes in expression of LIFR during embryonic neurulation.Thus,LIFR might play a specific role in neural tube development.All animal and human experimental procedures were approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS106K)on February 25,2016.
基金supported by the Scientific Research Projects of Inner Mongolia Power(Group)Co.,Ltd.(Internal Electric Technology(2021)No.3).
文摘This paper develops a real-time PV arrays maximum power harvesting scheme under partial shading condition(PSC)by reconfiguring PV arrays using Aquila optimizer(AO).AO is based on the natural behaviors of Aquila in capturing prey,which can choose the best hunting mechanism ingeniously and quickly by balancing the local exploitation and global exploration via four hunting methods of Aquila:choosing the searching area through high soar with the vertical stoop,exploring in different searching spaces through contour flight with quick glide attack,exploiting in convergence searching space through low flight with slow attack,and swooping through walk and grabbing prey.In general,PV arrays reconfiguration is a problem of discrete optimization,thus a series of discrete operations are adopted in AO to enhance its optimization performance.Simulation results based on 10 cases under PSCs show that the mismatched power loss obtained by AO is the smallest compared with genetic algorithm,particle swarm optimization,ant colony algorithm,grasshopper optimization algorithm,and butterfly optimization algorithm,which reduced by 4.34%against butterfly optimization algorithm.
基金supported by the National Natural Science Foundation(Spatial Distribution of Multi-environment Trial Stations for Maize Cultivar,41301075)the National Science-technology Support Plan Projects(Research and Demonstration of North China Corn Commercialized Breeding Technique,2014BAD01B01)Key Laboratory of Agricultural Information Acquisition Technology,Ministry of Agriculture
文摘Efficient evaluation of crop phenotypes is a prerequisite for breeding, cultivar adoption, genomics and phenomics study. Plant genotyping is developing rapidly through the use of high-throughput sequencing techniques,while plant phenotyping has lagged far behind and it has become the rate-limiting factor in genetics, large-scale breeding and development of new cultivars. In this paper,we consider crop phenotyping technology under three categories. The first is high-throughput phenotyping techniques in controlled environments such as greenhouses or specifically designed platforms. The second is a phenotypic strengthening test in semi-controlled environments, especially for traits that are difficult to be tested in multi-environment trials(MET), such as lodging, drought and disease resistance. The third is MET in uncontrolled environments, in which crop plants are managed according to farmer's cultural practices. Research and application of these phenotyping techniques are reviewed and methods for MET improvement proposed.
基金supported by the National Key Scientific Instruments and Equipment Development Project(2014YQ470377)National Special Fund for Agro-scientific Research in Public Interest(Grant No.201203052)+1 种基金Science and Technology Project of Beijing(Grant No.D131100000413002)China Agricultural University Education Foundation Dabeinong Education Funds(1081-2413001).
文摘False seeds can often be seen in the maize seed market,leading to a serious decline in maize yield.Those existing variety identification methods are expensive,time consuming,and destructive to seeds.The aim of this study is to develop a cheap,fast and non-destructive method which can robustly identify large amounts of maize seed varieties based on near-infrared reflectance spectroscopy(NIRS)and chemometrics.Because it is difficult to establish models for every variety in the market,this study mainly investigated the performance of models based on a large number of samples(more than 40 major varieties in the market).The reflectance spectra of maize seeds were collected by two modes(bulk kernels mode and single kernel mode).Both collection modes can be applied to identification,but only the single kernel mode can be applied to purity sorting.The spectra were pretreated with smoothing,the first derivative and vector normalization;and then principal component analysis(PCA),linear discriminant analysis(LDA)and biomimetic pattern recognition(BPR)were applied to establish identification models.The environmental factors such as producing areas and years have a significant influence on the performance of the models.Therefore,the method to improve the robustness of the models was investigated in this study.New indexes(correct acceptance degree(CAD),correct rejection degree(CRD)and correct degree(CD))were defined to analyze the performance of the models more accurately.Finally,the models obtained a mean correct discrimination rate of over 90%,and exhibited robust properties for samples harvested from different areas and years.The results showed that NIR technology combined with chemometrics methods such as PCA,LDA,and BPR could be a suitable and alternative technique to identify the authenticity of maize seed varieties.
基金grants from the Chinese Academy of Sciences(XDB27040201)the National Natural Science Foundation of China(91640114).
文摘Transposable elements(TEs)constitute the most abundant portions of plant genomes and can dramatically shape host genomes during plant evolution.They also play important roles in crop domestication.However,whether TEs themselves are also selected during crop domestication has remained unknown.Here,we identify an active long terminal repeat(LTR)retrotransposon,HUO,as a potential target of selection during rice domestication and breeding.HUO is a low-copy-number LTR retrotransposon,and is active under natural growth conditions and transmitted through male gametogenesis,preferentially inserting into genomic regions capable of transcription.HUO exists in all wild rice accessions and about half of the archaeological rice grains(1200–7000 years ago)and landraces surveyed,but is absent in almost all modern varieties,indicating its gradual elimination during rice domestication and breeding.Further analyses showed that HUO is subjected to strict gene silencing through the RNA-directed DNA methylation pathway.Our results also suggest that multiple HUO copies may trigger genomic instability through altering genome-wide DNA methylation and small RNA biogenesis and changing global gene expression,resulting in decreased disease resistance and yield,coinciding with its elimination during rice breeding.Together,our study suggests that negative selection of an active retrotransposon might be important for genome stability during crop domestication and breeding.