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大豆胞囊线虫病抗性候选基因GmRSCN-6克隆与表达分析 被引量:1
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作者 包冬芳 董海冉 +3 位作者 王俊 张婵娟 赵雪 韩英鹏 《中国油料作物学报》 CAS CSCD 北大核心 2020年第5期826-834,共9页
大豆胞囊线虫病(Heterodera glycines,Soybean cyst nematode,SCN)是世界性大豆病害之一,具有致病力强、传播范围广、休眠体(胞囊)存活时间长等特点。因此,大豆胞囊线虫病极难防治。筛选并利用抗病基因是加快大豆抗线育种进程的有效途... 大豆胞囊线虫病(Heterodera glycines,Soybean cyst nematode,SCN)是世界性大豆病害之一,具有致病力强、传播范围广、休眠体(胞囊)存活时间长等特点。因此,大豆胞囊线虫病极难防治。筛选并利用抗病基因是加快大豆抗线育种进程的有效途径之一。本文以东农L-10为材料,克隆GmRSCN-6基因并分析GmRSCN-6蛋白的性质,同时对该基因在抗感病品种进行表达分析。结果表明GmRSCN-6在SCN3号生理小种侵染10 d内逐渐上调表达。3 d时表达量出现小高峰,推测GmRSCN-6基因响应SCN3号生理小种入侵信号,10 d时GmRSCN-6基因的表达量达到最大,且抗病品种中的表达量远高于感病品种,表明GmRSCN-6基因参与大豆胞囊线虫病3号生理小种抗性反应。 展开更多
关键词 大豆 胞囊线虫 生物信息 基因功能鉴定
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Evaluation of Related Traits of GmTST2.1 and ZmGIF1 Genes in Soybean (Glycine Max)
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作者 dong hai-ran Chang Hong +3 位作者 Wang Jun Bao dong-fang Zhao Xue Han Ying-peng 《Journal of Northeast Agricultural University(English Edition)》 CAS 2020年第4期1-8,共8页
Soybean(Glycine max)is one of the most important food crops and oil crops in the world.According to the role of sucrose transporter in sugar accumulation,GmTST2.1(Glyma.04G000300)and ZmGIF1 of sugar transport related ... Soybean(Glycine max)is one of the most important food crops and oil crops in the world.According to the role of sucrose transporter in sugar accumulation,GmTST2.1(Glyma.04G000300)and ZmGIF1 of sugar transport related genes were separately overexpressed in the soybean cultivar Heihe 43 from the perspective of regulatory source to library relationship in the study.The function of soluble sugar accumulation in grains layed a theoretical foundation for the cultivation of new varieties of high-yield genetically modified soybeans.The results showed that the height and 100-seed weight of the over-expressed GmTST2.1 gene were increased with 7%and 17.7%and the soluble sugar content was increased with 1.575 times as much as that of the wild-type soybean.The overexpressed ZmGIF1 gene was found to be 10%higher than that of plant height,1.8%higher than that of 100-seed weight and larger seed size and 1.3 times higher than that of soluble sugar content.Biological yields were increased in both GmTST2.1 and ZmGIF1 genes. 展开更多
关键词 Glycine max soluble sugar GmTST2.1 gene ZmGIF1 gene
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