Objective To study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas. Methods Interleukin(IL)-2 and interferon-γ represent Th1 type c...Objective To study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas. Methods Interleukin(IL)-2 and interferon-γ represent Th1 type cytokines. IL-4, IL-6, IL-10, and IL-13 represent Th2 type cytokines. The gene expressions of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes, and glioma cell lines were detected by reverse transcription polymerase chain reaction (RT-PCR). The biological activity of cytokines in the supernatant of glioma cell lines was assayed by enzyme-linked immunosorbent assay (ELISA) method. Results The total positive rates of Th1 and Th2 type cytokines gene in human glioma cells were 14.77% and 75%. The total positive rates of Th1 and Th2 type cytokines gene in glioma infiltrating lymphocytes were 22.73% and 68.17%. There was obviously predominant expression of Th2 type cytokines in human glioma tissues, glioma infiltrating lymphocytes, and glioma cell lines. There was no unbalanced expression of Th1/Th2 cytokines in normal brain tissues. Conclusion There is a predominant expression of Th2 type cytokines in human glioma cells. The switching of Th1/Th2 cytokines gene may play an important role in the occurring and developing of human gliomas.展开更多
Objective To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats. Methods Seventy Sprague-Dawley rats were randomly divided into two gro...Objective To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats. Methods Seventy Sprague-Dawley rats were randomly divided into two groups, 35 rats in each. The control group sustained a one-minute clip compression (force of clip was 30 g) injury of the spinal cord at the T2 level, without X-irradiation. The experimental group received X-irradiation 14 days after injury. Neurological function was assessed by the modified Tarlov method, including hind limbs movement, inclined plane, and pain withdrawal. These tests were performed in a blinded fashion at 3, 7, 14, 21, 28, 35, and 42 days after injury. At 43 days after injury, histological examination of the injured spinal cord was performed following decapitation of the rats. Results Sixty-two rats met the experimental requirements (spinal cord injury was similar), 32 rats in experimental group and 30 rats in control group. Statistically significant difference was observed between the two groups in hind limbs movement and inclined plane (P<0.01), but not in the pain withdrawal test. The edema and necrosis areas of injured spinal cords in experimental group were less than those in control group, and axons in experimental group were significantly more than those in control group (P<0.01). Conclusion Delayed X-irradiation following spinal cord injury may enhance functional recovery by improving and restoring structural integrity of the injured spinal cord in rats.展开更多
Pr^3+-doped calcium niobium gallium garnet(Pr:CNGG)single crystals with different Pr^3+concentrations are successfully grown by the micro-pulling-down(μ-PD)method.The crystal structure,room-temperature absorption spe...Pr^3+-doped calcium niobium gallium garnet(Pr:CNGG)single crystals with different Pr^3+concentrations are successfully grown by the micro-pulling-down(μ-PD)method.The crystal structure,room-temperature absorption spectra,and fluorescence spectra of Pr:CNGG crystals are measured and discussed.The fluorescence results indicate their large dependence on the doping concentration.The fluorescence lifetime of the 1D2 energy level is also determined.The results indicate that Pr:CNGG crystal could be a potential solid-state laser gain medium.展开更多
Chronic internal carotid artery occlusion(CICAO)is a chronic cerebrovascular disease which,from various causes,leads to progressive stenosis of the internal carotid artery lumen and finally to complete occlusion.With ...Chronic internal carotid artery occlusion(CICAO)is a chronic cerebrovascular disease which,from various causes,leads to progressive stenosis of the internal carotid artery lumen and finally to complete occlusion.With an aging society,the detection rate of CICAO is increasing year by year,with the highest incidence of CICAO in elderly men.The main clinical manifestations of CICAO are ischemic stroke and transient ischemic attack.Drug therapy is the first choice for asymptomatic CICAO,and surgical treatment is an optional means of improving prognosis for symptomatic CICAO that cannot be controlled by drugs.The selection of indications needs further study.This article reviews the latest research progress in the epidemiology,etiology,clinical features,imaging analysis,and treatment of CICAO in order to guide clinical diagnosis and treatment.展开更多
文摘Objective To study the expression and switching of Th1/Th2 cytokines gene in human gliomas and its effects on occurring and developing of human gliomas. Methods Interleukin(IL)-2 and interferon-γ represent Th1 type cytokines. IL-4, IL-6, IL-10, and IL-13 represent Th2 type cytokines. The gene expressions of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes, and glioma cell lines were detected by reverse transcription polymerase chain reaction (RT-PCR). The biological activity of cytokines in the supernatant of glioma cell lines was assayed by enzyme-linked immunosorbent assay (ELISA) method. Results The total positive rates of Th1 and Th2 type cytokines gene in human glioma cells were 14.77% and 75%. The total positive rates of Th1 and Th2 type cytokines gene in glioma infiltrating lymphocytes were 22.73% and 68.17%. There was obviously predominant expression of Th2 type cytokines in human glioma tissues, glioma infiltrating lymphocytes, and glioma cell lines. There was no unbalanced expression of Th1/Th2 cytokines in normal brain tissues. Conclusion There is a predominant expression of Th2 type cytokines in human glioma cells. The switching of Th1/Th2 cytokines gene may play an important role in the occurring and developing of human gliomas.
文摘Objective To test the hypothesis that delayed X-irradiation can enhance the functional and structural recovery of the injured spinal cord in rats. Methods Seventy Sprague-Dawley rats were randomly divided into two groups, 35 rats in each. The control group sustained a one-minute clip compression (force of clip was 30 g) injury of the spinal cord at the T2 level, without X-irradiation. The experimental group received X-irradiation 14 days after injury. Neurological function was assessed by the modified Tarlov method, including hind limbs movement, inclined plane, and pain withdrawal. These tests were performed in a blinded fashion at 3, 7, 14, 21, 28, 35, and 42 days after injury. At 43 days after injury, histological examination of the injured spinal cord was performed following decapitation of the rats. Results Sixty-two rats met the experimental requirements (spinal cord injury was similar), 32 rats in experimental group and 30 rats in control group. Statistically significant difference was observed between the two groups in hind limbs movement and inclined plane (P<0.01), but not in the pain withdrawal test. The edema and necrosis areas of injured spinal cords in experimental group were less than those in control group, and axons in experimental group were significantly more than those in control group (P<0.01). Conclusion Delayed X-irradiation following spinal cord injury may enhance functional recovery by improving and restoring structural integrity of the injured spinal cord in rats.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFB0701002)the National Natural Science Foundation of China(Grant Nos.61621001 and 61605069)the MOE Key Laboratory of Advanced Micro-Structured Materials,China
文摘Pr^3+-doped calcium niobium gallium garnet(Pr:CNGG)single crystals with different Pr^3+concentrations are successfully grown by the micro-pulling-down(μ-PD)method.The crystal structure,room-temperature absorption spectra,and fluorescence spectra of Pr:CNGG crystals are measured and discussed.The fluorescence results indicate their large dependence on the doping concentration.The fluorescence lifetime of the 1D2 energy level is also determined.The results indicate that Pr:CNGG crystal could be a potential solid-state laser gain medium.
文摘Chronic internal carotid artery occlusion(CICAO)is a chronic cerebrovascular disease which,from various causes,leads to progressive stenosis of the internal carotid artery lumen and finally to complete occlusion.With an aging society,the detection rate of CICAO is increasing year by year,with the highest incidence of CICAO in elderly men.The main clinical manifestations of CICAO are ischemic stroke and transient ischemic attack.Drug therapy is the first choice for asymptomatic CICAO,and surgical treatment is an optional means of improving prognosis for symptomatic CICAO that cannot be controlled by drugs.The selection of indications needs further study.This article reviews the latest research progress in the epidemiology,etiology,clinical features,imaging analysis,and treatment of CICAO in order to guide clinical diagnosis and treatment.
