This study aimed to establish a rapid analytical method to determine antioxidants in essence. A simple,efficient and practical, vortex-assisted, cloud-point extraction（VACPE） procedure is proposed for extracting and...This study aimed to establish a rapid analytical method to determine antioxidants in essence. A simple,efficient and practical, vortex-assisted, cloud-point extraction（VACPE） procedure is proposed for extracting and pre-concentrating four different of synthetic phenolic antioxidants（SPAs）, propyl gallate（PG）, tert-butylhydroquinone（TBHQ）, butylated hydroxyanisole（BHA）, butylated hydroxytoluene（BHT） in essence prior to high performance liquid chromatography（HPLC） analysis. The non-ionic surfactant, fatty alcohol polyoxyethylene ether-9（AEO-9）, was used as extractant and vortex-mixing was utilized to reduce extraction time and improve extraction efficiency. The effective parameters of the extraction process, such as volume of extraction solvent, pH, vortex-mixing time, equilibration temperature and time, were optimized. Under the optimum conditions, the linear range of PG, TBHQ,BHA and BHT was 8.0–800 ng/mL. All correlation coefficients of the calibration curves were higher than0.996 and relative standard deviations（RSD, n = 5） were 2.36%–5.46%. The proposed method was successfully applied to the extraction and determination of antioxidants in essence samples with satisfactory relative recoveries of 89.4%–103.5%. The results confirmed the SPAs of essence could be effectively monitored by this method and also established good reference criteria for essence.展开更多
基金supported by College of Life Science and Technology, Kunming University of Science and Technology
文摘This study aimed to establish a rapid analytical method to determine antioxidants in essence. A simple,efficient and practical, vortex-assisted, cloud-point extraction（VACPE） procedure is proposed for extracting and pre-concentrating four different of synthetic phenolic antioxidants（SPAs）, propyl gallate（PG）, tert-butylhydroquinone（TBHQ）, butylated hydroxyanisole（BHA）, butylated hydroxytoluene（BHT） in essence prior to high performance liquid chromatography（HPLC） analysis. The non-ionic surfactant, fatty alcohol polyoxyethylene ether-9（AEO-9）, was used as extractant and vortex-mixing was utilized to reduce extraction time and improve extraction efficiency. The effective parameters of the extraction process, such as volume of extraction solvent, pH, vortex-mixing time, equilibration temperature and time, were optimized. Under the optimum conditions, the linear range of PG, TBHQ,BHA and BHT was 8.0–800 ng/mL. All correlation coefficients of the calibration curves were higher than0.996 and relative standard deviations（RSD, n = 5） were 2.36%–5.46%. The proposed method was successfully applied to the extraction and determination of antioxidants in essence samples with satisfactory relative recoveries of 89.4%–103.5%. The results confirmed the SPAs of essence could be effectively monitored by this method and also established good reference criteria for essence.
