期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Distinct roles of two RDL GABA receptors in fipronil action in the diamondback moth (Plutella xylostella) 被引量:1
1
作者 Ben-Jie Li Kun-Kun Wang +6 位作者 dong-pina chen Ying Yan Xu-Ling Cai Hui-Min chen Ke Dong Fei Lin Han-Hong Xu 《Insect Science》 SCIE CAS CSCD 2021年第6期1721-1733,共13页
The phenylpyrazole insecticide fipronil blocks resistance to dieldrin(RDL)γ-aminobutyric acid(GABA)receptors in insects,thereby impairing inhibitory neurotransmission.Some insect species,such as the diamondback moth(... The phenylpyrazole insecticide fipronil blocks resistance to dieldrin(RDL)γ-aminobutyric acid(GABA)receptors in insects,thereby impairing inhibitory neurotransmission.Some insect species,such as the diamondback moth(Plutella xylostella),possess more than one Rdl gene.The involvement of multiple Rdls in fipronil toxicity and resistance remains largely unknown.In this study,we investigated the roles of two Rdl genes,PxRdl1 and PxRdl2,in P.xylostella fipronil action.In Xenopus oocytes,RvRDL2 receptors were 40 times less sensitive to fipronil than P.vRDL1.P.vRDL2 receptors were also less sensitive to GABA compared with PxRDL1.Knockout of the fipronil-sensitive PxRdll reduced the fipronil potency 10-fold,whereas knockout of the fipronil-resistant PxRdl2 enhanced the fipronil potency 4.4-fold.Furthermore,in two fipronil-resistant diamondback moth field populations,PxRdl2 expression was elevated 3.7-and 4.1-fold compared with a susceptible strain,whereas PxRdl1 expression was comparable among the resistant and susceptible strains.Collectively,our results indicate antagonistic effects of PxRDLl and PxRDL2 on fipronil action in vivo and suggest that enhanced expression of fipronil-resistant PxRdl1 is potentially a new mechanism of fipronil resistance in insects. 展开更多
关键词 CRISPR-Cas9 FIPRONIL Plutella xylostella RDL
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部