Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata.Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus(TMV)by the co...Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata.Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus(TMV)by the conventional halfleaf method.Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis.The tested compounds showed moderate inactivation effect,whereas the lycoricidine derivatives showed good protective effect.The protective inhibitory activity of compounds L1(N-methyl-2,3,4-trimethoxyly-coricidine)(60.8%)and L3(N-methyl-2-methoxy-3,4-acetonidelycoricidine)(62.0%)was almost similar to the positive control,Ningnanmycin(66.4%).RT-PCR and Western-blot analysis displayed that compounds L1,L3,L5(N-allyl-2,3,4-triallyloxylycoricidine)exhibited antiviral activity,which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression.Additionally,defensive enzyme activities confirmed that compound L1 could increase the activity of PAL,POD,SOD to improve disease resistance of tobacco.展开更多
Four new diterpenoid alkaloids,nagaconitines A-D（1-4）,were isolated from the roots of Aconitum nagarum var.heterotrichum.Their structures were elucidated as 8-（4＇-hydroxylbutyryl） aconitine（1）,15-oxo-13-deoxylu...Four new diterpenoid alkaloids,nagaconitines A-D（1-4）,were isolated from the roots of Aconitum nagarum var.heterotrichum.Their structures were elucidated as 8-（4＇-hydroxylbutyryl） aconitine（1）,15-oxo-13-deoxyludaconitine（2）,15-hydroxyldelphisine（3）,and 14-hydroxyl-2- acetoxyspiradine C（4）based on extensive UV,IR,MS,1D NMR and 2D NMR analyses.Compounds 3-4 showed inhibition of cancer cell line SK-OV-3 with 31%（IC_（50） 43.78 μmol/L） and 24%（IC_（50） 32.14 μmol/L）,respectively.展开更多
基金the National Natural Science.Foundation of China(Nos.31770389,31470427,31270404).
文摘Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata.Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus(TMV)by the conventional halfleaf method.Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis.The tested compounds showed moderate inactivation effect,whereas the lycoricidine derivatives showed good protective effect.The protective inhibitory activity of compounds L1(N-methyl-2,3,4-trimethoxyly-coricidine)(60.8%)and L3(N-methyl-2-methoxy-3,4-acetonidelycoricidine)(62.0%)was almost similar to the positive control,Ningnanmycin(66.4%).RT-PCR and Western-blot analysis displayed that compounds L1,L3,L5(N-allyl-2,3,4-triallyloxylycoricidine)exhibited antiviral activity,which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression.Additionally,defensive enzyme activities confirmed that compound L1 could increase the activity of PAL,POD,SOD to improve disease resistance of tobacco.
基金supported by the National Natural Science Foundation of China (Nos. 21362045, 81560622)the project of Sciences and Technology Department of Yunnan Province (No. 2012CG007)
文摘Four new diterpenoid alkaloids,nagaconitines A-D（1-4）,were isolated from the roots of Aconitum nagarum var.heterotrichum.Their structures were elucidated as 8-（4＇-hydroxylbutyryl） aconitine（1）,15-oxo-13-deoxyludaconitine（2）,15-hydroxyldelphisine（3）,and 14-hydroxyl-2- acetoxyspiradine C（4）based on extensive UV,IR,MS,1D NMR and 2D NMR analyses.Compounds 3-4 showed inhibition of cancer cell line SK-OV-3 with 31%（IC_（50） 43.78 μmol/L） and 24%（IC_（50） 32.14 μmol/L）,respectively.
