Xiaoyan decoction inhibits tumor growth and improves the immunity of mouse with A549 lung carcinoma xenograft

Background:Previously,Xiaoyan decoction(XYD)has been found to have a potential anti-tumor effect in vitro.The aim of this study was to explore the effect of XYD in A549 lung carcinoma xenograft mouse model.Method:The A549 lung carcinoma xenograft mouse model were established and divided into control and experiment group.The dynamic growth of the xenograft tumors was observed and the immune cells such as T cell,regulatory T cell(Treg)were explored by Fluorescence-activated cell sorter(FACS)analysis.In this study,network pharmacology was used to screen the anti-cancer monomers in XYD,and their effect targets were also predicted.Result:The result showed that the tumor growth was inhibited and the percent of Treg was down-regulated(P<0.05).Ten drug monomers were screened to inhibit lung cancer cell proliferation and promote cell apoptosis through PI3K/Akt pathway.Conclusion:Our results suggested that XYD inhibited tumor growth and improved the immunity of A549 lung carcinoma xenograft mouse model.

Explain the relationship between the theory of spleen viscera and the pathogenesis of malignant tumors from the perspective of system theory

The spleen visceral manifestation theory(SVMT)is formed under the simple holistic thinking mode of traditional Chinese medicine(TCM).It emphasizes the change of our organic function.And SVMT can make me to understand the basic characteristics and rules of the organic functional change and disease evolution of spleen(which is a complex giant system from a macroscopic view).However,the formation of SVMT is still in the stage of fuzzy deduction,ignoring the structural basis of its function.As a result,although SVMT has a macroscopic accuracy,it is unable to analyze the internal structure from the perspective of reductionism.The systematic thought of unifying reductionism and holistic dialectics provides the possibility for the relationship between traditional Chinese medicine(TCM)theory and modern scientific research.Based on the systematic relationship between spleen storage and malignant tumor,this paper tries to take matter-energy-information as the starting point by drawing lessons from complex scientific theory and using the method of interdisciplinary comprehensive analysis under the guidance of system theory.Explore its mechanism and law more scientifically from the subcellular level,so as to further improve and deepen the theoretical system of malignant tumor research based on SVMT.