Pathological process of liver sinusoidal endothelial cells in liver diseases

Cirrhosis develops from liver fibrosis and is the severe pathological stage of all chronic liver injury. Cirrhosis caused by hepatitis B virus and hepatitis C virus infection is especially common. Liver fibrosis and cirrhosis involve excess production of extracellular matrix,which is closely related to liver sinusoidal endothelial cells(LSECs). Damaged LSECs can synthesize transforming growth factor-beta and platelet-derived growth factor,which activate hepatic stellate cells and facilitate the synthesis of extracellular matrix. Herein,we highlight the angiogenic cytokines of LSECs related to liver fibrosis and cirrhosis at different stages and focus on the formation and development of liver fibrosis and cirrhosis. Inhibition of LSEC angiogenesis and antiangiogenic therapy are described in detail. Targeting LSECs has high therapeutic potential for liver diseases. Further understanding of the mechanism of action will provide stronger evidence for the development of anti-LSEC drugs and new directions for diagnosis and treatment of liver diseases.

Oral Epidemic Diseases of Exposure Personnel in Long-term Low Dose Radiation

ORAL epidemic diseases of exposure personnel in long-term low-dose radiation yet have rarely been studied.Referred to WHO oral health survey method and symptom grading standard,data of 341 exposure persons in long-term low-dose radiation including o particle,β particle,and y rays,etc.,were collected from one camp in China in 2011 with cluster sampling and analyzed with Foxpro 6.0 and SPSS 16.0software.

miR-26a regulates mouse hepatocyte proliferation via directly targeting the 3' untranslated region of CCND2 and CCNE2

BACKGROUND： The deficiency of liver regeneration needs to be addressed in the fields of liver surgery, split liver transplan- tation and living donor liver transplantation. Researches of microRNAs would broaden our understandings on the mecha- nisms of various diseases. Our previous research confirmed that miR-26a regulated liver regeneration in mice; however, the relationship between miR-26a and its target, directly or in- directly, remains unclear. Therefore, the present study further investigated the mechanism of miR-26a in regulating mouse hepatocyte proliferation. METHODS： An established mouse liver cell line, Nctc-1469, was transfected with Ad5-miR-26a-EGFP, Ad5-anti-miR-26a- EGFP or AdS-EGFP vector. Cell proliferation was assessed by MTS, cell apoptosis and cell cycle by flow cytometry, and gene expression by Western blotting and quantitative real-time PCR. Dual-luciferase reporter assays were used to test targets of miR-26a. RESULTS： Compared with the Ad5-EGFP group, Ad5-anti- miR-26a-EGFP down-regulated miR-26a and increased prolif- eration of hepatocytes, with more cells entering the G1 phase of cell cycle （82.70%＋1.45% vs 75.80%＋_3.92%）, and decreased apoptosis （5.50%＋0.35% vs 6.73%_＋0.42%）. CCND2 and CCNE2 were the direct targeted genes of miR-26a, miR-26a down- regulation up-regulated CCND2 and CCNE2 expressions and down-regulated p53 expression in Nctc-1469 cells. On the con- trary, miR-26a over-expression showed the opposite results. CONCLUSIONS： miR-26a regulated mouse hepatocyte pro- liferation by directly targeting the 3＇ untranslated regions of cyclin D2/cyclin E2; miR-26a also regulated p53-mediated apoptosis. Our data suggested that miR-26a may be a promis- ing regulator in liver regeneration.

Liver transplantation using organs from deceased organ donors: a single organ transplant center experience

BACKGROUND： In 2011, a pilot program for deceased organ donation was initiated in China. We describe the first successful series of liver transplants in the pilot program.METHODS： From July 2011 to August 2012, our center performed 26 liver transplants from a pool of 29 deceased donors. All organ donation and allograft procurement were conducted according to the national protocol. The clinical data of donors and recipients were collected and summarized retrospectively.RESULTS： Among the 29 donors, 24 were China Category II donors（organ donation after cardiac death）, and five were China Category III donors（organ donation after brain death followed by cardiac death）. The recipients were mainly the patients with hepatocellular carcinoma. The one-year patient survival rate was 80.8% with a median follow-up of 422（2-696） days. Among the five mortalities during the follow-up,three died of tumor recurrence. In terms of post-transplant complications, 9 recipients（34.6%） experienced early allograft dysfunction, 1（3.8%） had non-anastomotic biliary stricture,and 1（3.8%） was complicated with hepatic arterial thrombosis.None of these complications resulted in patient death. Notably,primary non-function was not observed in any of the grafts.CONCLUSION： With careful donor selection, liver transplant from deceased donors can be performed safely and plays acritical role in overcoming the extreme organ shortage in China.

Study on application of spiral CT perfusion technology in the diagnosis of acute pancreatitis

Objective:To explore the application value of spiral CT perfusion technology in the diagnosis of acute pancreatitis (AP).Methods:A total of 78 patients with AP who were admitted in our hospital from September, 2014 to September, 2016 were included in the study. The velocity method was used to detect S-Amy and U-Amy. The emulsion enhancement velocity scattering turbidimetry was used to detect CRP. ELISA was used to detect D-D. The patients in the control group were performed with abdomen CT, while AP patients were performed with 64 slice spiral CT. The most integrated layer of pancreas display was regarded as the perfusion weighted imaging scanning layer, and CT perfusion scanning was performed. BF, BV, MTT, and PS were calculated.Results: S-Amy, U-Amy, CRP, and D-D in AP patients were significantly higher than those in the control group. With the disease progression, S-Amy and U-Amy were significantly reduced, while CRP and D-D were significantly elevated. BF and BV in AP patients were significantly lower than those in the control group, and those in SAP patients were significantly lower than those in MAP patients. With the elevation of CT grading, BF and BV were significantly reduced, while the comparison of MTT and PS among the various grading was not statistically significant.Conclusions:The pancreas perfusion in AP patients is in a low perfusion state. BF and BV are negatively correlated with the severity degree of AP, which can predict the prognosis. BF and BV in combined with the serum S-Amy, U-Amy, CRP, and D-D can provide a forceful evidence for the diagnosis, treatment, and condition evaluation of AP.

Role of MSCT and CRP detection in evaluating the severity of acute pancreatitis

Objective: To explore the role of MSCT and CRP detection in evaluating the severity of acute pancreatitis (AP). Methods: A total of 70 patients with AP who were admitted in our hospital from April, 2016 to April, 2017 were included in the study and divided into MAP group and SAP group with 35 cases in each group according to the clinical diagnosis and grading criteria of AP. Moreover, 35 healthy individuals who came for physical examination were served as the control group. The peripheral venous blood and urine were collected after admission. The enzyme dynamics method was used to detect AMY, UAMY, and CRP. The dynamic change of CRP was observed 1, 3, 5, and 7 d after admission. 16 spiral CT was used to study CRP change and CT grading. Results: AMY, UAMY, and CRP in MAP and SAP group were significantly higher than those in the control group. CRP level in SAP group was significantly higher than that in MAP group. CRP level was elevated with the increasing of clinical degree and CT grading. CRP level in D and E grades were significantly higher than those in grade A, B, and C grades. Conclusions: CT grading and CRP detection in patients with AP can accurately reflect the severity degree and prognosis, and the combined detection plays a vital role in the early diagnosis, condition evaluation and monitoring, and clinical treatment.