Interception of Layered LP-N and HLP-N at Ambient Conditions by Confined Template

We propose a feasible strategy of intercepting the layered polymeric nitrogen(LP-N)and hexagonal layered polymeric nitrogen(HLP-N)at ambient conditions by using the confinement templates.The stable mechanism of confined LP-N and HLP-N at ambient conditions is revealed.

Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

BACKGROUND Regular physical activity during childhood and adolescence is beneficial to bone development,as evidenced by the ability to increase bone density and peak bone mass by promoting bone formation.AIM To investigate the effects of exercise on bone formation in growing mice and to investigate the underlying mechanisms.METHODS 20 growing mice were randomly divided into two groups:Con group(control group,n=10)and Ex group(treadmill exercise group,n=10).Hematoxylin-eosin staining,immunohistochemistry,and micro-CT scanning were used to assess the bone formation-related indexes of the mouse femur.Bioinformatics analysis was used to find potential miRNAs targets of long non-coding RNA H19(lncRNA H19).RT-qPCR and Western Blot were used to confirm potential miRNA target genes of lncRNA H19 and the role of lncRNA H19 in promoting osteogenic differentiation.RESULTS Compared with the Con group,the expression of bone morphogenetic protein 2 was also significantly increased.The micro-CT results showed that 8 wk moderate-intensity treadmill exercise significantly increased bone mineral density,bone volume fraction,and the number of trabeculae,and decreased trabecular segregation in the femur of mice.Inhibition of lncRNA H19 significantly upregulated the expression of miR-149 and suppressed the expression of markers of osteogenic differentiation.In addition,knockdown of lncRNA H19 significantly downregulated the expression of autophagy markers,which is consistent with the results of autophagy-related protein changes detected in mouse femurs by immunofluorescence.CONCLUSION Appropriate treadmill exercise can effectively stimulate bone formation and promote the increase of bone density and bone volume in growing mice,thus enhancing the peak bone mass of mice.The lncRNA H19/miR-149 axis plays an important regulatory role in osteogenic differentiation.

Fe-substituted cobalt-phosphate polyoxometalates as enhanced oxygen evolution catalysts in acidic media

All-inorganic and earth-abundant bi-/trimetallic hydr(oxy)oxides are widely used as oxygen evolution electrocatalysts owing to their remarkable performance.However,their atomically precise structures remain undefined,complicating their optimization and limiting the understanding of their enhanced performance.Here,the underlying structure-property correlation is explored by using a well-defined cobalt-phosphate polyoxometalate cluster [{(Co4)(OH)3(PO4)}4(SiW9 O34)4]^32-(1),which may serve as a molecular model of multimetal hydr(oxy)oxides.The catalytic activity is enhanced upon replacing Co by Fe in 1,resulting in a reduced overpotential(385 mV) for oxygen evolution(by 66 mV) compared to that of the parent 1 at 10 mA cm^-2 in an acidic medium;this overpotential is comparable to that for the IrO2 catalyst These abundant-metal-based polyoxometalates exhibit high stability,with no evidence of degradation even after 24 h of operation.

Relationship between Methylation of FHIT and CDH13 Gene Promoter Region and Liver Cancer

In order to demonstrate the relationship between methylation of fragile histidine triad(FHIT)and T-cadherin/H-cadherin(CDH13)genes and liver cancer,the methylation status of FHIT and CDH13 was detected in healthy individuals and in Mongolian and Han patients with liver cancer.The phenol-chloroform method was used to extract genomic DNA.The methylation specific polymerase chain reaction method was applied to detect the methylation status of FHIT and CDH13.The relationship between smoking and alcohol consumption and gene(FHIT and CDH13)methylation was analyzed.There was significant difference in methylation rate of FHIT(72.67%,34.67%)and CDH13(72.0%,28.0%)between liver cancer patients and healthy individuals of Mongolian descent(P<0.05),as well as that of FHIT(68%,30.67%)and CDH13(64%,26%)between liver cancer patients and healthy individuals of Han individuals(P<0.05).There was also a relationship between smoking and drinking and the methylation of FHIT and CDH13(P<0.05).Thus,the methylation of FHIT and CDH13 had a relationship with liver cancer incidence.Smoking and alcohol ingestion may promote the methylation of FHIT and CDH13.