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MIR1868 negatively regulates rice cold tolerance at both the seedling and booting stages
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作者 Yang Shen Xiaoxi Cai +7 位作者 Yan Wang Wanhong li dongpeng li Hao Wu Weifeng Dong Bowei Jia Mingzhe Sun Xiaoli Sun 《The Crop Journal》 SCIE CSCD 2024年第2期375-383,共9页
Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive s... Low temperature causes rice yield losses of up to 30%–40%,therefore increasing its cold tolerance is a breeding target.Few genes in rice are reported to confer cold tolerance at both the vegetative and reproductive stages.This study revealed a rice-specific 24-nt miRNA,miR1868,whose accumulation was suppressed by cold stress.Knockdown of MIR1868 increased seedling survival,pollen fertility,seed setting,and grain yield under cold stress,whereas its overexpression conferred the opposite phenotype.Knockdown of MIR1868 increased reactive oxygen species(ROS)scavenging and soluble sugar content under cold stress by increasing the expression of peroxidase genes and sugar metabolism genes,and its overexpression produced the opposite effect.Thus,MIR1868 negatively regulated rice cold tolerance via ROS scavenging and sugar accumulation. 展开更多
关键词 RICE Cold tolerance MIRNA ROS scavenging Soluble sugar accumulation
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利用转基因毛状根高效培育大豆嵌合植株的方法
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作者 陈佳欣 梅浩 +6 位作者 黄彩翔 梁宗原 全依桐 李东鹏 布威麦尔耶姆·赛麦提 李欣欣 廖红 《植物学报》 CAS CSCD 北大核心 2024年第1期89-98,共10页
建立高效的大豆(Glycine max)转基因毛状根嵌合植株体系对于推动大豆功能基因组学研究具有重要意义。该研究利用3种大豆基因型材料比较了不同共培养条件下毛状根诱导率及成活率。结果显示,用发根农杆菌(Agrobacterium rhizogenes)侵染... 建立高效的大豆(Glycine max)转基因毛状根嵌合植株体系对于推动大豆功能基因组学研究具有重要意义。该研究利用3种大豆基因型材料比较了不同共培养条件下毛状根诱导率及成活率。结果显示,用发根农杆菌(Agrobacterium rhizogenes)侵染外植体并在黑暗条件下共培养1天是诱导毛状根形成的有效策略。研究发现清除下胚轴处不定根可显著增加毛状根的数目并促进根系生长,进而提高转基因毛状根的阳性率。毛状根诱导14天接种根瘤菌,可增强生长初期转基因毛状根与根瘤菌的接触,从而提高大豆的结瘤效率。该研究成功建立了一种高效培育大豆转基因毛状根嵌合植株的方法,可广泛应用于大豆基因功能研究。 展开更多
关键词 大豆 毛状根 嵌合植株 结瘤 优化
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Tensor coupling effect on relativistic symmetries
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作者 ShouWan Chen dongpeng li JianYou Guo 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS CSCD 2016年第8期34-42,共9页
The similarity renormalization group is used to transform the Dirac Hamiltonian with tensor coupling into a diagonal form. The upper(lower) diagonal element becomes a Schr¨odinger-like operator with the tensor co... The similarity renormalization group is used to transform the Dirac Hamiltonian with tensor coupling into a diagonal form. The upper(lower) diagonal element becomes a Schr¨odinger-like operator with the tensor component separated from the original Hamiltonian.Based on the operator, the tensor effect of the relativistic symmetries is explored with a focus on the single-particle energy contributed by the tensor coupling. The results show that the tensor coupling destroying(improving) the spin(pseudospin) symmetry is mainly attributed to the coupling of the spin-orbit and the tensor term, which plays an opposite role in the single-particle energy for the(pseudo-) spin-aligned and spin-unaligned states and has an important influence on the shell structure and its evolution. 展开更多
关键词 similarity renormalization group tensor relativistic symmetries
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