Cell proliferation during hair cell regeneration induced by Math1 in vestibular epithelia in vitro

Hair cell regeneration is the fundamental method of correcting hearing loss and balance disorders caused by hair cell damage or loss. How to promote hair cell regeneration is a hot focus in current research. In mammals, cochlear hair cells cannot be regenerated and few vestibular hair cells can be renewed through spontaneous regeneration. However, Math1 gene transfer allows a few inner ear cells to be transformed into hair cells in vitro or in vivo. Hair cells can be renewed through two possible means in birds： supporting cell differentiation and transdifferentiation with or without cell division. Hair cell regeneration is strongly associated with cell proliferation. Therefore, this study explored the relationship between Math1-induced vestibular hair cell regeneration and cell division in mammals. The mouse vestibule was isolated to harvest vestibular epithelial cells. Ad-Math1-enhanced green fluorescent protein （EGFP） was used to track cell division during hair cell transformation.5-Bromo-2′-deoxyuridine （BrdU） was added to track cell proliferation at various time points. Immunocytochemistry was utilized to determine cell differentiation and proliferation. Results demonstrated that when epithelial cells were in a higher proliferative stage, more of these cells differentiated into hair cells by Math1 gene transfer. However, in the low proliferation stage, no BrdU-positive cells were seen after Math1 gene transfer. Cell division always occurred before Math1 transfection but not during or after Math1 transfection, when cells were labeled with BrdU before and after Ad-Math1-EGFP transfection. These results confirm that vestibular epithelial cells with high proliferative potential can differentiate into new hair cells by Math1 gene transfer, but this process is independent of cell proliferation.

Optimal Scheduling for Flexible Regional Integrated Energy Systemwith Soft Open Point

The Regional Integrated Energy System(RIES)has brought new modes of development,utilization,conversion,storage of energy.The introduction of Soft Open Point(SOP)and the application of Power to Gas(P2G)technology will greatly deepen the coupling of the electricity-gas integrated energy system,improve the flexibility and safety of the operation of the power system,and bring a deal of benefits to the power system.On this background,an optimal dispatch model of RIES combined cold,heat,gas and electricity with SOP is proposed.Firstly,RIES architecture with SOP and P2G is designed and its mathematical model also is built.Secondly,on the basis of considering the optimal scheduling of combined cold,heat,gas and electricity,the optimal scheduling model for RIES was established.After that,the original model is transformed into a mixed-integer second-order cone programming model by using linearization and second-order cone relaxation techniques,and the CPLEX solver is invoked to solve the optimization problem.Finally,the modified IEEE 33-bus systemis used to analyze the benefits of SOP,P2G technology and lithium bromide absorption chillers in reducing systemnetwork loss and cost,as well as improving the system’s ability to absorb wind and solar and operating safety.