Human induced pluripotent stem cells （iPSCs） hold great promise for regenerative med- icine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononu- clear cells （UCBMCs） is of great sig...Human induced pluripotent stem cells （iPSCs） hold great promise for regenerative med- icine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononu- clear cells （UCBMCs） is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs （UCB-iPSCs） have characteristics that are identical to pluripotent human embryonic stem cells （hESCs）. This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.展开更多
Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug scree...Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug screening and developmental biology studies. However, integrating retroviral （Takahashi et al., 2007） or lentiviral （Hockemeyer et al., 2008） gene delivery systems and the exogenous oncogene c-Myc （Takahashi et al., 2007; Hockemeyer et al., 2008） have been typically used in the reprogramming process, which could increase risks of insertional mutations or epigenetic abnormalities and conse- quently pose biosecurity concerns for basic research and clinical applications of hiPSCs.展开更多
基金supported in part by grants from China National Basic Research Program of China(Grant No.2012CBA01301 to QZ,2013CB967102 to LL and 2012CB966500 to XZ)the ‘‘Strategic Priority Research Program’’ of the Chinese Academy of Sciences(Grant No.XDA01030101)to LW
文摘Human induced pluripotent stem cells （iPSCs） hold great promise for regenerative med- icine. Generating iPSCs from immunologically immature newborn umbilical cord blood mononu- clear cells （UCBMCs） is of great significance. Here we report generation of human iPSCs with great efficiency from UCBMCs using a dox-inducible lentiviral system carrying four Yamanaka factors. We generated these cells by optimizing the existing iPSC induction protocol. The UCBMC-derived iPSCs （UCB-iPSCs） have characteristics that are identical to pluripotent human embryonic stem cells （hESCs）. This study highlights the use of UCBMCs to generate highly functional human iPSCs that could accelerate the development of cell-based regenerative therapy for patients suffering from various diseases.
基金supported by grants from the National Basic Research Program of China (No. 2013CB967102)the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA01030506)the International S&T Cooperation Program of China (No. 2013DFG30680)
文摘Human induced pluripotent stem cells （hiPSCs） can self-renew indefinitely and have the potential to differentiate into all cell types in the human body, thus hold great promise in regen- erative medicine, drug screening and developmental biology studies. However, integrating retroviral （Takahashi et al., 2007） or lentiviral （Hockemeyer et al., 2008） gene delivery systems and the exogenous oncogene c-Myc （Takahashi et al., 2007; Hockemeyer et al., 2008） have been typically used in the reprogramming process, which could increase risks of insertional mutations or epigenetic abnormalities and conse- quently pose biosecurity concerns for basic research and clinical applications of hiPSCs.
