Immunogenic cell death-related long noncoding RNA influences immunotherapy against lung adenocarcinoma

Lung adenocarcinoma(LUAD)is the leading cause of cancer-related deaths,accounting for over a million deaths worldwide annually.Immunogenic cell death(ICD)elicits an adaptive immune response.However,the role of ICD-related long noncoding RNAs(lncRNAs)in LUAD is unknown.In this study,we investigated the characteristics of the tumor microenvironment in LUAD,the prognostic significance of ICD-related lncRNAs,and the half-maximal inhibitory concentration(IC50)of possible chemotherapeutic drugs.We sorted prognostic lncRNAs using univariate Cox regression and constructed a risk signature based on them.We then confirmed the model’s accuracy and generated a nomogram.Additionally,we performed immune microenvironment analysis,somatic mutation calculation,Tumor Immune Dysfunction and Exclusion(TIDE)analysis,and anticancer pharmaceutical IC50 prediction.Least absolute shrinkage and selection operator Cox regression identified 27 prognostic lncRNAs related to ICD,and a unique risk signature using 10 ICD-related lncRNAs was constructed.The risk score was confirmed to be a reliable predictor of survival,with the highest c-index score.The signature had a remarkable predictive performance with clinical applicability and could accurately predict the overall survival in LUAD.Furthermore,the lncRNA signature was closely associated with immunocyte invasion.We also analyzed the correlation between the risk score,tumor-infiltrating immune cells,and prognosis and identified high immune and ESTIMATE scores in low-risk patients.Moreover,we observed elevated checkpoint gene expression and low TIDE scores in high-risk patients,indicating a good immunotherapy response.Finally,high-risk patients were shown to be susceptible to anticancer medications.Therefore,our unique risk signature comprising 10 ICD-related lncRNAs was demonstrated to indicate the characteristics of the tumor-immune microenvironment in LUAD,predict patients’overall survival,and guide individualized treatment.

Image Features of Retinal Astrocytic Hamartoma in a Patient with Tuberous Sclerosis Complex

Purpose:.To describe the image features of retinal astrocytic hamartoma in a 35-year-old male patient with tuberous sclerosis complex(TSC).Methods :. Fundus photography,. fundus autofluorescence(FAF), fundus fluorescein angiography(FFA), and spectral domain optical coherence tomography.(SD-OCT).were performed for this retinal astrocytic hamartoma.Results: Fundus photography showed that the retinal astrocytic hamartoma presented as a well-circumscribed,.mulberry-like lesion consisting of glistening yellowish spherules of calcification..FAF demonstrated dense hyper-autofluorescence spots corresponding to retinal astrocytic hamartoma..FFA revealed leakage from dilated retinal capillaries over the hamartoma.SD-OCT indicated moth-eaten optically empty spaces and hyperreflective dots within the lesion..The lesion surface was fluctuate.Conclusion: FAF is a useful imaging modality for obtaining greater contrast between a retinal astrocytic hamartoma and the surrounding retina due to hyper-autofluorescence of calcification in the lesion. FFA is beneficial for monitoring the abnormal blood vessels in these lesions. SD-OCT is capable of visualization the structural details, such as the uneven surface and inner hyperreflective dots.