Modern medicine has made remarkable achievements in safeguarding people’s life and health,however,it is increasingly found that in the face of complex diseases,selective targeting of single target is often difficult ...Modern medicine has made remarkable achievements in safeguarding people’s life and health,however,it is increasingly found that in the face of complex diseases,selective targeting of single target is often difficult to produce a comprehensive rehabilitation effect,and is prone to induce drug resistance,toxic side effects.Traditional Chinese medicine(TCM)has a long history of clinical application,and its clinical value in the treatment of complex diseases such as cardiovascular and cerebrovascular diseases,digestive diseases,skin diseases,rheumatism and immunity diseases,and adjuvant treatment of tumors has been proven to have obvious advantages.However,its modern research is relatively lagging behind,and in the face of the aging society and the characteristics of the modern disease spectrum,the traditional knowledge-driven research paradigm seems to be stuck in a bottleneck and difficult to make greater breakthroughs.Focusing on the key issues of TCM development in the new era,the clinical value-oriented strategy becomes to be a new research paradigm of TCM inheritance and innovation development,and dominant diseases would be the focus of the TCM inheritance and innovation development,which has been highly valued in recent years by the TCM academia and the relevant national management departments.Based on the clinical value,a series of policies are formulated for the selection and evaluation of the TCM dominant diseases(TCMDD),and exploratory researches about the clinical efficacy characteristics,the modern scientific connotation interpretation were carried out.The clinical value-oriented research paradigm of TCMDD inheritance and innovation development has been initially formed,which is characterized by strong policy support as the guarantee,systematic and standardized selection and evaluation methods as the driving force,scientific and effective research on internal mechanisms as the expansion,and effective clinical guidelines and principles as the transformation,which is of great value in promoting the high-quality development of the industries and undertaking of TCM.In this paper,the main policy support,selection and evaluation methods,therapeutic effect characterization,and modern scientific connotation research strategies of TCMDD in recent years have been comprehensively sorted out,with a view to providing the healthy and benign development of the research on TCMDD.展开更多
In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources,fingerprint and genetic diversity analysis of 52 castor bean materials from 12 reg...In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources,fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers(FMs)associated with fatty acid metabolism-related genes.A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103–0.695.Shannon’s information index(I),observed heterozygosity(Ho)and expected heterozygosity(He)were 0.699,0.188 and 0.436 respectively.The clustering results indicated that the castor germplasm could be divided into two groups with the genetic similarity coefficient of 0.59.The genetic similarity of 12 regions ranged from 0.518 to 0.917 and the genetic distance was between 0.087 and 0.658.A total of 5 pairs of core primers were screened to construct a digital fingerprint of different castor germplasm resources,which could distinguish all 52 germplasms.This study provides a scientific basis for screening high-quality castor germplasm resources and broadening the genetic basis of castor breeding at the molecular level.展开更多
Cognitive dysfunction is one of the common central nervous systems(CNS)complications of diabetes mellitus,which seriously affects the quality of life of patients and results in a huge economic burden.The glymphatic sy...Cognitive dysfunction is one of the common central nervous systems(CNS)complications of diabetes mellitus,which seriously affects the quality of life of patients and results in a huge economic burden.The glymphatic system dysfunction mediated by aquaporin-4(AQP4)loss or redistribution in perivascular astrocyte endfeet plays a crucial role in diabetes-induced cognitive impairment(DCI).However,the mechanism of AQP4 loss or redistribution in the diabetic states remains unclear.Accumulating evidence suggests that peripheral insulin resistance target tissues and CNS communication affect brain homeostasis and that exosomal miRNAs are key mediators.Glucose and lipid metabolism disorder is an important pathological feature of diabetes mellitus,and skeletal muscle,liver and adipose tissue are the key target insulin resistance organs.In this review,the changes in exosomal miRNAs induced by peripheral metabolism disorders in diabetes mellitus were systematically reviewed.We focused on exosomal miRNAs that could induce low AQP4 expression and redistribution in perivascular astrocyte endfeet,which could provide an interorgan communication pathway to illustrate the pathogenesis of DCI.Furthermore,the mechanisms of exosome secretion from peripheral insulin resistance target tissue and absorption to the CNS were summarized,which will be beneficial for proposing novel and feasible strategies to optimize DCI prevention and/or treatment in diabetic patients.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.82174112).
文摘Modern medicine has made remarkable achievements in safeguarding people’s life and health,however,it is increasingly found that in the face of complex diseases,selective targeting of single target is often difficult to produce a comprehensive rehabilitation effect,and is prone to induce drug resistance,toxic side effects.Traditional Chinese medicine(TCM)has a long history of clinical application,and its clinical value in the treatment of complex diseases such as cardiovascular and cerebrovascular diseases,digestive diseases,skin diseases,rheumatism and immunity diseases,and adjuvant treatment of tumors has been proven to have obvious advantages.However,its modern research is relatively lagging behind,and in the face of the aging society and the characteristics of the modern disease spectrum,the traditional knowledge-driven research paradigm seems to be stuck in a bottleneck and difficult to make greater breakthroughs.Focusing on the key issues of TCM development in the new era,the clinical value-oriented strategy becomes to be a new research paradigm of TCM inheritance and innovation development,and dominant diseases would be the focus of the TCM inheritance and innovation development,which has been highly valued in recent years by the TCM academia and the relevant national management departments.Based on the clinical value,a series of policies are formulated for the selection and evaluation of the TCM dominant diseases(TCMDD),and exploratory researches about the clinical efficacy characteristics,the modern scientific connotation interpretation were carried out.The clinical value-oriented research paradigm of TCMDD inheritance and innovation development has been initially formed,which is characterized by strong policy support as the guarantee,systematic and standardized selection and evaluation methods as the driving force,scientific and effective research on internal mechanisms as the expansion,and effective clinical guidelines and principles as the transformation,which is of great value in promoting the high-quality development of the industries and undertaking of TCM.In this paper,the main policy support,selection and evaluation methods,therapeutic effect characterization,and modern scientific connotation research strategies of TCMDD in recent years have been comprehensively sorted out,with a view to providing the healthy and benign development of the research on TCMDD.
基金National Natural Science Foundation of China,(31271759)Guangdong Provincial Science and Technology Projects(2013B060400024,2014A020208116 and 2016A020208015)(China)Project of Enhancing School with Innovation of Guangdong Ocean University(GDOU2013050206)(China).
文摘In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources,fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers(FMs)associated with fatty acid metabolism-related genes.A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103–0.695.Shannon’s information index(I),observed heterozygosity(Ho)and expected heterozygosity(He)were 0.699,0.188 and 0.436 respectively.The clustering results indicated that the castor germplasm could be divided into two groups with the genetic similarity coefficient of 0.59.The genetic similarity of 12 regions ranged from 0.518 to 0.917 and the genetic distance was between 0.087 and 0.658.A total of 5 pairs of core primers were screened to construct a digital fingerprint of different castor germplasm resources,which could distinguish all 52 germplasms.This study provides a scientific basis for screening high-quality castor germplasm resources and broadening the genetic basis of castor breeding at the molecular level.
基金supported by the National Natural Science Foundation of China(No.82174112)。
文摘Cognitive dysfunction is one of the common central nervous systems(CNS)complications of diabetes mellitus,which seriously affects the quality of life of patients and results in a huge economic burden.The glymphatic system dysfunction mediated by aquaporin-4(AQP4)loss or redistribution in perivascular astrocyte endfeet plays a crucial role in diabetes-induced cognitive impairment(DCI).However,the mechanism of AQP4 loss or redistribution in the diabetic states remains unclear.Accumulating evidence suggests that peripheral insulin resistance target tissues and CNS communication affect brain homeostasis and that exosomal miRNAs are key mediators.Glucose and lipid metabolism disorder is an important pathological feature of diabetes mellitus,and skeletal muscle,liver and adipose tissue are the key target insulin resistance organs.In this review,the changes in exosomal miRNAs induced by peripheral metabolism disorders in diabetes mellitus were systematically reviewed.We focused on exosomal miRNAs that could induce low AQP4 expression and redistribution in perivascular astrocyte endfeet,which could provide an interorgan communication pathway to illustrate the pathogenesis of DCI.Furthermore,the mechanisms of exosome secretion from peripheral insulin resistance target tissue and absorption to the CNS were summarized,which will be beneficial for proposing novel and feasible strategies to optimize DCI prevention and/or treatment in diabetic patients.
