Electrospun Core-Shell Fibrous 2D Scaffold with Biocompatible Poly(Glycerol Sebacate) and Poly-l-Lactic Acid for Wound Healing

Biomimetic scaffolds made by synthetic materials are usually used to replace the natural tissues aimed at speeding up the skin regeneration.In this study,a flexible and cytocompatible poly(glycerol sebacate)@poly-l-lactic acid(PGS@PLLA)fibrous scaffold with a core-shell structure was fabricated by coaxial electrospinning,where the shell PLLA was used to be a skeleton with pores on the fibrous surface.The fibrous morphology with pores on the surface of the prepared fibers was observed by SEM.The core-shell microstructure of PGS@PLLA fibers was confirmed by TEM and Laser Scanning Confocal Microscopy(LSCM).In addition,the prepared fibers exhibited a strong ability to repair tissues of the skin wound,where the stability of cell security and proliferation,and the lower inflammatory response were all superior to those of pure PLLA scaffold.It’s worth noting that the percentage of skin tissue was regenerated by 95%within 14 days,which suggests the potential application for electrospun-based synthetic fibrous scaffolds on wound healing.

Aligned polymer fibers produced via an additive electric field

Electrospinning is known to be a highly versatile method to produce nanofibers,and several techniques have been developed to align nanofibers.In this paper,poly(vinyl alcohol)(PVA),poly(vinylpyrrolidone)(PVP),poly(propylene carbonate)(PC),poly(ethylene oxide)(PEO),PVA/Chitosan and PVA/Fe_(3)O_(4) uniaxially aligned ultrafine fibers were obtained with electrospinning method by adding another electric field in the collection area.Alignment of the nanofibers was characterized by the use of digital cameras and field emission scanning electron microscopy,polarized Fourier transform infrared spectroscopy(FTIR),and wideangle X-ray diffraction(XRD).The mechanism of fiber alignment was investigated as well.

Electrospun composite nanofibrous membrane as wound dressing with good adhesion

A photopolymerizationable mimic mussel protein structure monomer,dopamine methacrylamide(DMA),was synthesized.The photopolymerization of DMA was analysed by series real time near infrared spectroscopy(SRTIR).Dopamine methacrylamide/poly(ethylene oxide)(DMA/PEO)nanofibers were successfully prepared by electrospinning of aqueous DMA/PEO solution.Biocompatible nanofibrous membrane with good adhesion was produced by photocuring from the DMA/PEO nanofibers.The surface characterization and structure of the composite nanofibrous membrane were characterized by a scanning electron microscopy(SEM)and contact angle measurements.For identifying the potential crystalline of curing,a XRD method was used through comparing diffraction data.In the cell adhesion test we utilized the mouse fibroblast(L929)to exam the various use of the nanofibrous membrane as scaffolding materials for skin regeneration.

Preparation of chitosan/ alginate microcapsules by high-voltage electrostatic method

Chitosan and sodium alginate have the opposite charges;they can become a gelatin by the electrostatic attraction,High-voltage electrostatic droplet generator method was used to prepare chitosan-sodium alginate microcapsule.Multi-layer chitosan-sodium alginate microcapsule was prepared through layer-by-layer self-assembly,and the morphology was investigated.In addition,the release property of ofloxacin in microcapsules was studied by UV-Vis microscopy under different conditions such as pH value,layer number,etc.The results showed that the prepared microcapsules have a smooth surface with average particle size about 100μm.The result of controlled release indicated that the prepared microcapsules are pH-independent,and the rate of release decreased when the layer number increases.