Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality timber.However,most of these species have difficulties surviving in vitro...Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality timber.However,most of these species have difficulties surviving in vitro cultivation.Active chlorine and sealing systems are often used to reduce contamination and increase gas exchange.The aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of E.grandis × E.urophylla.Two clones(C1 and C2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication phases.Three sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation phase.Two luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious rooting.Active chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro establishment.Active chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication phase.There were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an autoclave.The increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.展开更多
Correction to:J.For.Res.https://doi.org/10.1007/s11676-020-01240-5 In the original publication of the article,there were errors in the fig 3 and fig 6.The corrected Figs.3 and 6 are given below:The original article ha...Correction to:J.For.Res.https://doi.org/10.1007/s11676-020-01240-5 In the original publication of the article,there were errors in the fig 3 and fig 6.The corrected Figs.3 and 6 are given below:The original article has been corrected.展开更多
基金the support of the Coordination for the Improvement of Higher Education Personnel-Brazil (CAPES)the National Council for Scientific and Technological Development (CNPq)the Research Support Foundation of the State of Minas (FAPEMIG)。
文摘Hybrid combinations of Eucalyptus have increased due to expansion of plantations into unconventional areas and to the search for higher quality timber.However,most of these species have difficulties surviving in vitro cultivation.Active chlorine and sealing systems are often used to reduce contamination and increase gas exchange.The aim of the present study is to evaluate the establishment,multiplication,elongation and adventitious rooting of E.grandis × E.urophylla.Two clones(C1 and C2) and four active chlorine concentrations(0.000%,0.001%,0.003%,and 0.005%) were tested in the establishment and multiplication phases.Three sealing forms(W/M,1/M and 3/M) and the same four active chlorine concentrations were applied to the elongation phase.Two luminosities(dark and light)and three sealings(W/M,1/M and 3/M) were tested during adventitious rooting.Active chlorine concentration of0.005% led to the lowest fungal contamination rate and to the highest in vitro establishment.Active chlorine concentration of 0.003% resulted in the greatest length and highest number of shoots per explant in the multiplication phase.There were no phytotoxicity problems and the quality of plants grown in an environment with active chlorine was maintained in comparison with those grown in an autoclave.The increase in gas exchange in ventilation systems had a positive impact on the in vitro growth and development of plants.
文摘Correction to:J.For.Res.https://doi.org/10.1007/s11676-020-01240-5 In the original publication of the article,there were errors in the fig 3 and fig 6.The corrected Figs.3 and 6 are given below:The original article has been corrected.
