目的观察在大鼠慢性脑低灌注(chronic cerebral hypoperfusion,CCH)模型中海马神经血管单元变化的特点,探讨海马神经血管单元变化与CCH致大鼠学习记忆受损的关系。方法取220~250 g SPF级8周龄SD雄性大鼠44只,按随机数字表法分为CCH组(n=...目的观察在大鼠慢性脑低灌注(chronic cerebral hypoperfusion,CCH)模型中海马神经血管单元变化的特点,探讨海马神经血管单元变化与CCH致大鼠学习记忆受损的关系。方法取220~250 g SPF级8周龄SD雄性大鼠44只,按随机数字表法分为CCH组(n=24)和对照组(n=20)。CCH组通过永久性结扎双侧颈总动脉建立CCH模型,对照组不结扎。各组大鼠分别于术后4、12周通过旷场实验、物体辨别实验和Morris水迷宫实验检测自主探索行为反应和学习记忆功能,双重免疫荧光标记检测大鼠海马星形胶质细胞足突水通道蛋白-4(aquaporin-4,AQP4)和血管基底膜CollagenⅣ的共表达,透射电镜观察大鼠海马神经血管单元超微结构改变。结果与对照组比较,4周和12周CCH组大鼠:(1)物体辨别指数明显下降[(0.86±0.14)vs(0.71±0.16),P<0.05;(0.83±0.19)vs(0.64±0.10),P<0.05],定位航行及空间探索逃避潜伏期均显著延长(P<0.05);(2)海马星形胶质细胞足突AQP4[(1.94±0.80)vs(1.10±0.41),P<0.05;(2.05±0.51)vs(0.96±0.50),P<0.01]、血管基底膜CollagenⅣ[(6.90±2.42)vs(4.25±1.01),P<0.05;(6.63±2.20)vs(3.66±1.22),P<0.01]表达显著降低,二者的共表达比例[(0.94±0.02)vs(0.91±0.01),P<0.01;(0.91±0.01)vs(0.89±0.01),P<0.01]均明显减少;(3)超微结构显示海马星形胶质细胞足突与血管基底膜明显解离,胶质血管连接及神经血管单元明显破坏。结论慢性脑低灌注所致大鼠持续性学习记忆功能受损可能与海马神经血管单元的破坏有关。展开更多
The Langmuir-Blodgett (LB) technique was used for the first time to study inhibition mechanism. Stearic acid Langmuir-Blodgett (SALB) monolayers showed a good inhibition effect on iron corrosion in neutral environment...The Langmuir-Blodgett (LB) technique was used for the first time to study inhibition mechanism. Stearic acid Langmuir-Blodgett (SALB) monolayers showed a good inhibition effect on iron corrosion in neutral environment and the inhibition is mainly based on the blocking effect.展开更多
The electrochemistry of di-μ-oxo-dimanganese complex was investigated. It was found that no redox peak was observed in the cyclic voltammogram (CV) of the complex at the bare gold electrode, but at thiouracil-modifie...The electrochemistry of di-μ-oxo-dimanganese complex was investigated. It was found that no redox peak was observed in the cyclic voltammogram (CV) of the complex at the bare gold electrode, but at thiouracil-modified gold electrode, a pair of redox peaks were observed showing that thiouracil can promote the proton-coupled electron transfer reaction of the complex.展开更多
The electrochemical behaviours of three kinds of soluble polyimides were investigated for the first time. It was observed that the cyclic voltammograms(CVS) of these polyimides in nonaqueous solutions are obviously di...The electrochemical behaviours of three kinds of soluble polyimides were investigated for the first time. It was observed that the cyclic voltammograms(CVS) of these polyimides in nonaqueous solutions are obviously different from that of the poly-imide films casted on surfaces of glass carbon electrode(GCEs) in aqueous solutions.展开更多
文摘目的观察在大鼠慢性脑低灌注(chronic cerebral hypoperfusion,CCH)模型中海马神经血管单元变化的特点,探讨海马神经血管单元变化与CCH致大鼠学习记忆受损的关系。方法取220~250 g SPF级8周龄SD雄性大鼠44只,按随机数字表法分为CCH组(n=24)和对照组(n=20)。CCH组通过永久性结扎双侧颈总动脉建立CCH模型,对照组不结扎。各组大鼠分别于术后4、12周通过旷场实验、物体辨别实验和Morris水迷宫实验检测自主探索行为反应和学习记忆功能,双重免疫荧光标记检测大鼠海马星形胶质细胞足突水通道蛋白-4(aquaporin-4,AQP4)和血管基底膜CollagenⅣ的共表达,透射电镜观察大鼠海马神经血管单元超微结构改变。结果与对照组比较,4周和12周CCH组大鼠:(1)物体辨别指数明显下降[(0.86±0.14)vs(0.71±0.16),P<0.05;(0.83±0.19)vs(0.64±0.10),P<0.05],定位航行及空间探索逃避潜伏期均显著延长(P<0.05);(2)海马星形胶质细胞足突AQP4[(1.94±0.80)vs(1.10±0.41),P<0.05;(2.05±0.51)vs(0.96±0.50),P<0.01]、血管基底膜CollagenⅣ[(6.90±2.42)vs(4.25±1.01),P<0.05;(6.63±2.20)vs(3.66±1.22),P<0.01]表达显著降低,二者的共表达比例[(0.94±0.02)vs(0.91±0.01),P<0.01;(0.91±0.01)vs(0.89±0.01),P<0.01]均明显减少;(3)超微结构显示海马星形胶质细胞足突与血管基底膜明显解离,胶质血管连接及神经血管单元明显破坏。结论慢性脑低灌注所致大鼠持续性学习记忆功能受损可能与海马神经血管单元的破坏有关。
基金The project is supported by National Natural Science Foundation of China Corrosion Science Laboratory, Chinese Academy of Sciences.
文摘The Langmuir-Blodgett (LB) technique was used for the first time to study inhibition mechanism. Stearic acid Langmuir-Blodgett (SALB) monolayers showed a good inhibition effect on iron corrosion in neutral environment and the inhibition is mainly based on the blocking effect.
文摘The electrochemistry of di-μ-oxo-dimanganese complex was investigated. It was found that no redox peak was observed in the cyclic voltammogram (CV) of the complex at the bare gold electrode, but at thiouracil-modified gold electrode, a pair of redox peaks were observed showing that thiouracil can promote the proton-coupled electron transfer reaction of the complex.
基金The project is supprted by NationI Natural Science Poundation ot Chin and Statomnl.
文摘The electrochemical behaviours of three kinds of soluble polyimides were investigated for the first time. It was observed that the cyclic voltammograms(CVS) of these polyimides in nonaqueous solutions are obviously different from that of the poly-imide films casted on surfaces of glass carbon electrode(GCEs) in aqueous solutions.
