Omega-3 polyunsaturated fatty acids （w-3 PUFAs） are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mamm...Omega-3 polyunsaturated fatty acids （w-3 PUFAs） are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce w-3 PUFAs, due to the lack of the co-3 fatty acid desaturase gene （fat-1 gene）. Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-l. We have demonstrated that degree of codon bias of Brat-1 gene was in- creased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the w-6/co-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver （P〈0.05）, and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection （P〈0.05）. This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of w-6 PUFAs to e0-3 PUFAs.展开更多
A new ant-algorithm-based routing approach is proposed for the VC routing problem with considering the comprehensive effect between the resource utilization and the load balance in ATM networks. In the approach, the b...A new ant-algorithm-based routing approach is proposed for the VC routing problem with considering the comprehensive effect between the resource utilization and the load balance in ATM networks. In the approach, the backup paths are calculated first, and then an ant algorithm based on the ability of ants to find the shortest path between their nest and the food source during their searching food, is constructed to optimize the VC global route. Simulation results show that the proposed approach can realize VC routing effectively according to the current traffic states in the networks and the user-specified delay requirements.展开更多
基金funded by the National Key Project for Production of Transgenic Breeding Plan, China (2013ZX08007002, 2014ZX08007-002)the Earmarked Fund for ModernAgro-Industry Technology Research System, China (CARS38)+1 种基金the Agricultural Science and Technology Innovation Program (ASTIP-IAS03)the Fundamental Research Budget Increment Project (2013ZL031) of Chinese Academy of Agricultural Sciences
文摘Omega-3 polyunsaturated fatty acids （w-3 PUFAs） are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce w-3 PUFAs, due to the lack of the co-3 fatty acid desaturase gene （fat-1 gene）. Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-l. We have demonstrated that degree of codon bias of Brat-1 gene was in- creased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the w-6/co-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver （P〈0.05）, and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection （P〈0.05）. This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of w-6 PUFAs to e0-3 PUFAs.
基金Supported by Shanghai Post & Telecom Administration-SJTU Information and Telecom Joint Lab Project(1999-GR1-3)
文摘A new ant-algorithm-based routing approach is proposed for the VC routing problem with considering the comprehensive effect between the resource utilization and the load balance in ATM networks. In the approach, the backup paths are calculated first, and then an ant algorithm based on the ability of ants to find the shortest path between their nest and the food source during their searching food, is constructed to optimize the VC global route. Simulation results show that the proposed approach can realize VC routing effectively according to the current traffic states in the networks and the user-specified delay requirements.
