A new chitosan-supported borohydride reducing reagent (CBER) was prepared by treatment of KBH4 with the resin of chitosan derivative, which was first synthesized from: the reaction of cross-linked chitosan microsphere...A new chitosan-supported borohydride reducing reagent (CBER) was prepared by treatment of KBH4 with the resin of chitosan derivative, which was first synthesized from: the reaction of cross-linked chitosan microsphere with glycidyl trimethylammonium chloride. CBER could reduce aromatic carbonyl compound to corresponding alcohol.展开更多
An automated flow-injection stopped-flow micellar enhanced Spectrofluorimetric method for determination of L-ascorbic acid (AA) is described. The method is based on the interference of laccase by AA. causing a decreas...An automated flow-injection stopped-flow micellar enhanced Spectrofluorimetric method for determination of L-ascorbic acid (AA) is described. The method is based on the interference of laccase by AA. causing a decrease in the rate of the laccase-catalysed fluorescence reaction in the presence of Brij-35. The resulting change of fluorescence intensity at em 530nm (ex430nm) is proportional to the concentration of AA with the linear range over 0. 2 to 2. 0μg/ml. The significant interference from ferric and cupric ions can be eliminated by addition of EDTA. The method has been applied to the determination of AA in pharmaceuticals. the results agree well with the nominal values and those obtained by the reference method. The recovery of AA in pharmaceuticals are between 91.5% and 115.4%.展开更多
基金the National Natural Science Foundation of China (No.29977014).
文摘A new chitosan-supported borohydride reducing reagent (CBER) was prepared by treatment of KBH4 with the resin of chitosan derivative, which was first synthesized from: the reaction of cross-linked chitosan microsphere with glycidyl trimethylammonium chloride. CBER could reduce aromatic carbonyl compound to corresponding alcohol.
文摘An automated flow-injection stopped-flow micellar enhanced Spectrofluorimetric method for determination of L-ascorbic acid (AA) is described. The method is based on the interference of laccase by AA. causing a decrease in the rate of the laccase-catalysed fluorescence reaction in the presence of Brij-35. The resulting change of fluorescence intensity at em 530nm (ex430nm) is proportional to the concentration of AA with the linear range over 0. 2 to 2. 0μg/ml. The significant interference from ferric and cupric ions can be eliminated by addition of EDTA. The method has been applied to the determination of AA in pharmaceuticals. the results agree well with the nominal values and those obtained by the reference method. The recovery of AA in pharmaceuticals are between 91.5% and 115.4%.
