Objective:To analyze the sequence of ORFl protein of Torque teno virus to prepare for the future hyhrid experiments.Methods:The sequence of ORFl protein of Torque teno vims was analyzed by bioinformalics using some we...Objective:To analyze the sequence of ORFl protein of Torque teno virus to prepare for the future hyhrid experiments.Methods:The sequence of ORFl protein of Torque teno vims was analyzed by bioinformalics using some web lools.Results:The most likely cleavage site was between position 14aa and 15aa and signal peptide may be position laa-14aa.Two possible transmembrane helices from inside to outside and three possible transmembrane helices from outside to inside were found.The position 509(NKTN) was the potential:V-glyeosylation site. The speculative molecular weight of TTV ORF1 protein,which may be a kind of unstable protein was 88 705.7 Da.laa-91aa and 278aa-361aa were localized in non-regular secondary structure region.Conclusions:TTV ORF1 protein may be a nuclear protein which contains two nonregular secondary structure region.265aa to 486aa and 510aa to 679aa may be the two approciale fragments to construct the plasmids,which would be prepared for the future hybrid experiments to study the functional positions of the protein and the interactions between TTV and its hosts. Bioinformatics analysis would possibly make it easier to study the protein’s function.展开更多
基金supported by a grant from a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions
文摘Objective:To analyze the sequence of ORFl protein of Torque teno virus to prepare for the future hyhrid experiments.Methods:The sequence of ORFl protein of Torque teno vims was analyzed by bioinformalics using some web lools.Results:The most likely cleavage site was between position 14aa and 15aa and signal peptide may be position laa-14aa.Two possible transmembrane helices from inside to outside and three possible transmembrane helices from outside to inside were found.The position 509(NKTN) was the potential:V-glyeosylation site. The speculative molecular weight of TTV ORF1 protein,which may be a kind of unstable protein was 88 705.7 Da.laa-91aa and 278aa-361aa were localized in non-regular secondary structure region.Conclusions:TTV ORF1 protein may be a nuclear protein which contains two nonregular secondary structure region.265aa to 486aa and 510aa to 679aa may be the two approciale fragments to construct the plasmids,which would be prepared for the future hybrid experiments to study the functional positions of the protein and the interactions between TTV and its hosts. Bioinformatics analysis would possibly make it easier to study the protein’s function.
