Effects of Different Wall-breaking Methods on Extraction of Solanesol from Stems and Leaves of Potato

In order to extract solanesol from potato stems and leaves more effectively and improve the extraction rate of solanesol, the same batch of potato stems and leaves harvested from Guyuan, Ningxia was selected as a research object, cell wall of potato stems and leaves was broken by enzymolysis with cellulase and high- speed shearing, and then reflux-extracted with 95% ethanol. Solanesol content was determined by HPLC, and extract yield was calculated. Extract yield and solanesol extraction rate was used as an index for comparison of difference between the 2 wall-breaking methods, so as to select the optimal wall-breaking method. The results showed that enzymolysis with cellulase exhibited extraction rate and extract yield of solanesol of 91.38% and 8.02%, respectively, which were better than those under high-speed shear emulsification technique. The enzymolysis wall-breaking method has the advantages of simple operation and strong feasibility.

Study on pharmacokinetics and tissue distribution of the isocorydine derivative (AICD) in rats by HPLC-DAD method

A simple and effective high-performance liquid chromatography with diode-array detection method coupled with a liquid-liquid extraction pretreatment has been developed for determining the pharmacokinetics and tissue distribution of a novel structurally modified derivative(8-acetaminoisocorydine) of isocorydine.According to the in vivo experiments data calculations by DAS 2.0 software,a two-compartment metabolic model was suitable for describing the pharmacokinetic of 8-acetaminoisocorydine in rats.8-Acetamino-isocorydine was absorbed well after oral administration,and the absolute bioavailability was 76.5%.The half-life of 8-acetamino-isocorydine after intravenous and oral administration was 2.2 h and 2.0 h,respectively.In vivo,8-acetamino-isocorydine was highly distributed in the lungs,kidney and liver;however,relatively little entered the brain,suggesting that 8-acetaminoisocorydine could not easily pass through the blood brain barrier.Our work describes the first characterization of the pharmacokinetic parameters and tissue distribution of 8-acetamino-isocorydine.The acquired data will provide useful information for the in vivo pharmacology of 8-acetaminoisocorydine,and can be applied to new drug research.

Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum

The water-soluble polysaccharide LBP-1 was isolated and characterized from Lycium barbarum L.LBP-1 was mainly composed of arabinose,galactose,glucose,xylose,mannose at a molar ratio of 37.53:28.08:14.72:7.83:4.50,respectively.Based on nuclear magnetic resonance(NMR)and methylation analysis,the backbone of LBP-1 was speculated in theα-L-Ara(1→[5-α-L-Ara(1→3)-β-D-Galp-(1→]n→4)-α-D-Galp-(1[→5-α-L-Ara(1]n[→6)-β-D-Galp-(1→4)-β-D-Galp-(1→]n,and the side chains of LBP-1 were in theα-L-Ara(1→3)-β-D-Galp-(1→6 position.These results showed that LBP-1 inhibited the growth of cancer A549 cells through cell cycle arrest and apoptosis,with an IC50 value of 42.5μg/mL.In addition,LBP-1 altered the expression of Cyclin D1,Cyclin D3,and CDK 2,thus blocking the cell cycle in G0/G1 phase,reducing cell migration,and regulating the PI3K/Akt/mTOR signaling pathway to induce apoptosis.