MicroRNAs(miRNA)are recently discovered endogenous,small noncoding RNAs(of 22 nucleotides)that play pivotal roles in gene regulation.They are involved in post-transcriptional control of gene expression.miRNAs are emer...MicroRNAs(miRNA)are recently discovered endogenous,small noncoding RNAs(of 22 nucleotides)that play pivotal roles in gene regulation.They are involved in post-transcriptional control of gene expression.miRNAs are emerging as important regulators of cell proliferation,development,cancer formation,stress responses,cell death and physiological conditions.Increasing evidence has demonstrated the human miRNAs bind to their target mRNA sequences with perfect or near-perfect sequence complementarily.This provides a powerful strategy for discovering potential type 2 diabetes mellitus(T2DM)targets and gives the probability to exploit them for diagnostic and therapeutic causes.About 6%of the world population is affected by T2DM,and it is recognized as a global epidemic by the World Health Organization.At present there is no valid biomarker to control or manage T2DM.Therefore,the present study applied a mature sequence of miRNAs from publicly accessible databases to identify the miRNA from T2DM expressed sequence tags,and the results are detailed and discussed below.展开更多
Genetic,environmental and demographic factors contribute to the development of essential hypertension.Genetic polymorphism of Rennin-angiotensin-aldosterone system(RAAS)has been extensively studied to determine the ge...Genetic,environmental and demographic factors contribute to the development of essential hypertension.Genetic polymorphism of Rennin-angiotensin-aldosterone system(RAAS)has been extensively studied to determine the genetic susceptibility to hypertension.The insertion/deletion(I/D)angiotensin converting enzyme(ACE)polymorphism has been established as a cardiovascular risk factor in some population,but its association with essential hypertension is controversial.This study sought to determine the association of I/D polymorphism of the ACE gene in south Indian essential hypertensive subjects.A total of 208 clinically diagnosed essential hypertensive patients without any associated diseases and 220 healthy control subjects were included in this study.Distribution and allelic frequency of Insertion(I)and Deletion(D)polymorphism at the 287 base pair Alu repeat sequence in the intron 16 of ACE gene were analyzed.The distribution of II,ID,DD genotypes of ACE gene was 28.3%,32.6%and 38.9%respectively in essential hypertensive patients and to 53.6%,26.3%and 20%in controls.The allele frequency for D allele is 0.58 in essential hypertension as compared to 0.34 of control subjects.The genotype and allele frequency of ACE gene polymorphism is significantly differed in patients when compared to controls.In conclusion,the I/D polymorphism of ACE gene is associated with Indian essential hypertension.展开更多
The discovery of microRNAs （miRNAs） is one of tbe most significant breakthroughs in recent decades, miRNAs are a class of small, endogenous, non-coding RNAs （19-24 nt） that negatively regulate the expression of ta...The discovery of microRNAs （miRNAs） is one of tbe most significant breakthroughs in recent decades, miRNAs are a class of small, endogenous, non-coding RNAs （19-24 nt） that negatively regulate the expression of target genes by binding to their 3＇ untranslated region and are involved in various biological processes. Three main approaches that are globally used for the detection of miRNA include direct cloning, forward genetics and computational approaches using bioinformatics.展开更多
Irrespective of positive developments of cancer treatment,the mortality due to various cancers remains high and the mechanisms of cancer initiation and the development also remains mysterious.As we know that microRNAs...Irrespective of positive developments of cancer treatment,the mortality due to various cancers remains high and the mechanisms of cancer initiation and the development also remains mysterious.As we know that microRNAs are considered to be a short noncoding RNA molecules consisting of 21e25 nucleotides(nt)in length and they silence their target genes by inhibiting mRNA translation or degrading the mRNA molecules by binding to their 30-untranslated(UTR)region and play a very important role in cancer biology.Recent evidences indicate that miR-21 is over expressed in cancer stem cells and plays a vital role in cell proliferation,apoptosis,and invasion.Even though an increased expression level of miR-21 has been observed in cancer stem cells,studies related to the role of miR-21 in cancer stem cells are limited.The main aim of this mini review is to explain the potency of miR-21 in various cancer stem cells(CSCs)and as a new target for therapeutic interventions of cancer progression.展开更多
文摘MicroRNAs(miRNA)are recently discovered endogenous,small noncoding RNAs(of 22 nucleotides)that play pivotal roles in gene regulation.They are involved in post-transcriptional control of gene expression.miRNAs are emerging as important regulators of cell proliferation,development,cancer formation,stress responses,cell death and physiological conditions.Increasing evidence has demonstrated the human miRNAs bind to their target mRNA sequences with perfect or near-perfect sequence complementarily.This provides a powerful strategy for discovering potential type 2 diabetes mellitus(T2DM)targets and gives the probability to exploit them for diagnostic and therapeutic causes.About 6%of the world population is affected by T2DM,and it is recognized as a global epidemic by the World Health Organization.At present there is no valid biomarker to control or manage T2DM.Therefore,the present study applied a mature sequence of miRNAs from publicly accessible databases to identify the miRNA from T2DM expressed sequence tags,and the results are detailed and discussed below.
文摘Genetic,environmental and demographic factors contribute to the development of essential hypertension.Genetic polymorphism of Rennin-angiotensin-aldosterone system(RAAS)has been extensively studied to determine the genetic susceptibility to hypertension.The insertion/deletion(I/D)angiotensin converting enzyme(ACE)polymorphism has been established as a cardiovascular risk factor in some population,but its association with essential hypertension is controversial.This study sought to determine the association of I/D polymorphism of the ACE gene in south Indian essential hypertensive subjects.A total of 208 clinically diagnosed essential hypertensive patients without any associated diseases and 220 healthy control subjects were included in this study.Distribution and allelic frequency of Insertion(I)and Deletion(D)polymorphism at the 287 base pair Alu repeat sequence in the intron 16 of ACE gene were analyzed.The distribution of II,ID,DD genotypes of ACE gene was 28.3%,32.6%and 38.9%respectively in essential hypertensive patients and to 53.6%,26.3%and 20%in controls.The allele frequency for D allele is 0.58 in essential hypertension as compared to 0.34 of control subjects.The genotype and allele frequency of ACE gene polymorphism is significantly differed in patients when compared to controls.In conclusion,the I/D polymorphism of ACE gene is associated with Indian essential hypertension.
文摘The discovery of microRNAs （miRNAs） is one of tbe most significant breakthroughs in recent decades, miRNAs are a class of small, endogenous, non-coding RNAs （19-24 nt） that negatively regulate the expression of target genes by binding to their 3＇ untranslated region and are involved in various biological processes. Three main approaches that are globally used for the detection of miRNA include direct cloning, forward genetics and computational approaches using bioinformatics.
文摘Irrespective of positive developments of cancer treatment,the mortality due to various cancers remains high and the mechanisms of cancer initiation and the development also remains mysterious.As we know that microRNAs are considered to be a short noncoding RNA molecules consisting of 21e25 nucleotides(nt)in length and they silence their target genes by inhibiting mRNA translation or degrading the mRNA molecules by binding to their 30-untranslated(UTR)region and play a very important role in cancer biology.Recent evidences indicate that miR-21 is over expressed in cancer stem cells and plays a vital role in cell proliferation,apoptosis,and invasion.Even though an increased expression level of miR-21 has been observed in cancer stem cells,studies related to the role of miR-21 in cancer stem cells are limited.The main aim of this mini review is to explain the potency of miR-21 in various cancer stem cells(CSCs)and as a new target for therapeutic interventions of cancer progression.
