Trimethoprim(TMP)is an antibiotic frequently detected in various environments.Microorganisms are the main drivers of emerging antibiotic contaminant degradation in the environment.However,the feasibility and stability...Trimethoprim(TMP)is an antibiotic frequently detected in various environments.Microorganisms are the main drivers of emerging antibiotic contaminant degradation in the environment.However,the feasibility and stability of the anaerobic biodegradation of TMP with sulfate as an electron acceptor remain poorly understood.Here,TMP-degrading microbial consortia were successfully enriched from municipal activated sludge(AS)and river sediment(RS)as the initial inoculums.The acclimated consortia were capable of transforming TMP through demethylation,and the hydroxyl-substituted demethylated product(4-desmethyl-TMP)was further degraded.The biodegradation ofTMP followed a 3-parameter sigmoid kinetic model.The potential degraders(Acetobacterium,Desulfovibrio,Desulfbbulbus,and unidentified Peptococcaceae)and fermenters(Lentimicrobium and Petrimonas)were significantly enriched in the acclimated consortia.The AS-and RS-acclimated TMP-degrading consortia had similar core microbiomes.The anaerobic biodegradation ofTMP could be coupled with sulfate respiration,which gives new insights into the antibiotic fate in real environments and provides a new route for the bioremediation of antibiotic-contaminated environments.展开更多
In this paper we present a designated verifier-set signature(DVSS),in which the signer allows to designate many verifiers rather than one verifier,and each designated verifier can verify the validity of signature by h...In this paper we present a designated verifier-set signature(DVSS),in which the signer allows to designate many verifiers rather than one verifier,and each designated verifier can verify the validity of signature by himself.Our research starts from identity-based aggregator(IBA)that compresses a designated set of verifier’s identities to a constant-size random string in cryptographic space.The IBA is constructed by mapping the hash of verifier’s identity into zero or pole of a target curve,and extracting one curve’s point as the result of aggregation according to a specific secret.Considering the different types of target curves,these two IBAs are called as zeros-based aggregator and poles-based aggregator,respectively.Based on them,we propose a practical DVSS scheme constructed from the zero-pole cancellation method which can eliminate the same elements between zeros-based aggregator and poles-based aggregator.Due to this design,our DVSS scheme has some distinct advantages:(1)the signature supporting arbitrary dynamic verifiers extracted from a large number of users;and(2)the signature with short and constant length.We rigorously prove that our DVSS scheme satisfies the security properties:correctness,consistency,unforgeability and exclusivity.This is a preview of subscription content,log in to check access.展开更多
基金This study was supported by the National Natural Science Foundation of China(Grant No.51808537)the China Postdoctoral Science Foundation(No.2019M650866)+3 种基金the Key Research Program of the Chinese Academy of Sciences(No.KFZD-SW-219)the Youth Technology Fund Project of Gansu Province(No.18JR3RA023)the Provincial Science and Technology Plan Projects of Gansu Province(No.2015017)the Youth Science and Technology Foundation of Gansu Province(No.1506RJYA154).
文摘Trimethoprim(TMP)is an antibiotic frequently detected in various environments.Microorganisms are the main drivers of emerging antibiotic contaminant degradation in the environment.However,the feasibility and stability of the anaerobic biodegradation of TMP with sulfate as an electron acceptor remain poorly understood.Here,TMP-degrading microbial consortia were successfully enriched from municipal activated sludge(AS)and river sediment(RS)as the initial inoculums.The acclimated consortia were capable of transforming TMP through demethylation,and the hydroxyl-substituted demethylated product(4-desmethyl-TMP)was further degraded.The biodegradation ofTMP followed a 3-parameter sigmoid kinetic model.The potential degraders(Acetobacterium,Desulfovibrio,Desulfbbulbus,and unidentified Peptococcaceae)and fermenters(Lentimicrobium and Petrimonas)were significantly enriched in the acclimated consortia.The AS-and RS-acclimated TMP-degrading consortia had similar core microbiomes.The anaerobic biodegradation ofTMP could be coupled with sulfate respiration,which gives new insights into the antibiotic fate in real environments and provides a new route for the bioremediation of antibiotic-contaminated environments.
基金The work was supported by the National Key Technologies R&D Programs of China(2018YFB1402702 and 2017YFB0802500)the“13th”Five-Year National Cryptographic Development Foundation(MMJJ20180208)+1 种基金NSFC-Genertec Joint Fund For Basic Research(U1636104)the National Natural Science Foundation of China(Grant Nos.61572132,61972032 and U1705264).
文摘In this paper we present a designated verifier-set signature(DVSS),in which the signer allows to designate many verifiers rather than one verifier,and each designated verifier can verify the validity of signature by himself.Our research starts from identity-based aggregator(IBA)that compresses a designated set of verifier’s identities to a constant-size random string in cryptographic space.The IBA is constructed by mapping the hash of verifier’s identity into zero or pole of a target curve,and extracting one curve’s point as the result of aggregation according to a specific secret.Considering the different types of target curves,these two IBAs are called as zeros-based aggregator and poles-based aggregator,respectively.Based on them,we propose a practical DVSS scheme constructed from the zero-pole cancellation method which can eliminate the same elements between zeros-based aggregator and poles-based aggregator.Due to this design,our DVSS scheme has some distinct advantages:(1)the signature supporting arbitrary dynamic verifiers extracted from a large number of users;and(2)the signature with short and constant length.We rigorously prove that our DVSS scheme satisfies the security properties:correctness,consistency,unforgeability and exclusivity.This is a preview of subscription content,log in to check access.
