Effect of Distance of Sanitary Pits on the Microbial and Heavy Metal Levels in Hand Dug Well Water Samples Consumed by People Living in Akwuke, Enugu South Local Government Area of Enugu State

Studies were carried out to assess the effect of distance of sanitary pits on the microbial and heavy metal levels in hand dug well water samples consumed by people living in Akwuke, Enugu South Local government area of Enugu State, using standard biochemical and spectrophotometric analysis. The well water samples were digested with aqua-regia and four heavy metals (Pb, Cd, Cu and Zn) were assayed in the well water samples at sampling distances of 3, 6 and 10 m respectively from the sanitary pits. The range of mean bacterial counts (cfu/g) for the four detected pathogenic bacteria; S. aureus, Klebsiella pnemeoniae, Echerichia coli and Salmonella enteritidis were 83 - 3730, 510 - 870, 50 - 2535 and 240 - 3420 Cfu/g at sampling distances of 3, 6 and 10 m respectively from the sanitary pits. The mean bacteria counts of the four detected pathogenic organisms in the well water sample at sampling distances of 3 and 6 m respectively from the sanitary pits were above the WHO recommended threshold limits for a safe drinking water. The mean bacterial counts of Salmonella enteritidis in the well water samples of sampling distance of 10 m from the sanitary pits was above the WHO recommended permissible limits. At sampling distances of 3, 6 and 10 m from the sanitary pits, the mean range of Pb, Cd, Cu and Zn in the well water samples were, 0.03 - 0.3, 0.02 - 0.05, 0.46 - 1.71 and 1.63 - 7.03 μg/g respectively. The mean levels of Pb and Cd in the well water samples at sampling distances of 3 and 6 m respectively from the sanitary pits were above their respective WHO recommended threshold limits. The mean heavy metals (Cd, Pb, Cu and Zn) in the well water samples at sampling distances of 3, 6, and 10 m respectively from the sanitary pits were statistically significant at p < 0.05.

Effect of Distance of Sanitary Pits on the Physicochemical Properties of Hand Dug Well Water Samples Consumed by People Living in Akwuke Community, Enugu South Local Government Area of Enugu State

Studies were carried out to evaluate the effect of distance of sanitary pits on the physicochemical properties of hand-dug well water samples consumed by people living in Akwuke community, Enugu South Local Government Area of Enugu State, using standard analytical procedures and instrumentation. The mean range of pH, turbidity, electrical conductivity, total dissolved solids, nitrate and sulphate in the well water samples at the determined sampling distances of 3, 6 and 10 m from the sanitary pits were 5.9 - 7.24, 1.92 - 6.63 NTU, 122.03 - 303.91 μs/cm, 100.00 - 261.90 mg/L, 1.21 - 4.68 mg/L and 28.89 - 49/61 mg/L respectively. The investigated mean physicochemical parameters of the well water samples were generally observed to increase with decrease in the distance of the well water samples from the sanitary pits. The levels of the determined physicochemical parameter of the well water samples at sampling distances of 3, 6, and 10 m respectively from the sanitary pits were statistically significant. The mean pH values of the well water samples at sampling distances of 3 and 6 m respectively from the sanitary pits were below the WHO recommended threshold limits for safe drinking water. Additionally, the mean values of turbidity and total dissolved solids of the well water samples at a sampling distance of 3 m from the sanitary pits were above the WHO permissible limits. The pH, turbidity and total dissolved solids are some of the most important parameters used in assessing the quality of any water source, therefore, the poor physicochemical properties of well water samples at a sampling distance of 3 m from the sanitary pits could put at severe health risk consumption of this water over a prolonged time. Siting well water at a reasonable far distance (above 10 m) for sanitary pits especially in communities with severe water scarcity would definitely help guarantee access to safe drinking water by rural as well as urban dwellers in Nigeria.

Comparative Assessment of the Phytochemical and Selected Heavy Metal Levels in <i>Cucumis sativus</i>L. and <i>Solanum aethiopicum</i>L. Fruit Sample Grown in South Eastern and North Central Regions of Nigeria Respectively

Studies were carried out to comparatively assess the phytochemical and heavy metal levels in Solanum aethiopicum L. and Cucumis sativus L. fruit samples grown in the South Eastern and North Central regions of Nigeria respectively using standard analytical procedures and instrumentation. The fruit samples were assayed for selected heavy metals (Pb, Cd and Cu) using atomic absorption spectrophotometer after wet digestion of the samples. The five detected phytochemicals (flavonoids, alkaloids, terpenoids, saponins and glycosides) were present at varying amounts in the investigated fruit samples from the South Eastern and North Central regions of Nigeria. The range of mean values of flavonoids, terpenoids, saponins and glycosides in the S. aethiopicum L. fruit samples from the two studied regions were 0.50 - 0.57, 0.53 - 1.26, 0.44 - 0.78, 1.12 - 1.93 and 0.40 - 0.50 mg/g respectively. The range of mean values of flavonoids, alkaloids, terpenoids, saponins and glycosides in the C. sativus L. fruit samples from the two studied regions of Nigeria were 1.27 - 1.69, 0.53 - 0.55, 0.96 - 1.51, 0.41 - 0.83 and 0.90 - 1.74 mg/g respectively. The range of mean vales of Pb, Cd, and Cu in the S. aethiopicum L. fruit samples from the two studied regions were 0.15 - 0.24, 0 - 0.01, 0.78 - 1.12 μg/g respectively. 0.31 - 0.40, 0.02 - 0.05 and 0.62 - 0.96 μg/g were the range of mean values of Pb, Cd and Cu respectively in the C. sativus L. fruit samples from the two studied regions of Nigeria. Of the three investigated heavy metals, only Cd was at toxic levels in the C. sativus L. fruit samples from the South Eastern and North Central regions of Nigeria. This is therefore a health concern to the fruit consumers that includes these fruits in their habitual daily fruit diets. Although the therapeutic and pharmaceutical benefits that would be derived from consuming, the investigated fruit samples has been shown in this study, there is therefore a possible risk of undue exposure to environmental pollutants such as heavy metal, especially through growing these fruits in polluted soils resulting from unwholesome anthropogenic practices. For fruit consumers to derive maximum health benefits from consuming these fruits, pollutants like heavy metals must be at non-toxic levels and this can only be achieved by ensuring that these fruits samples are grown and harvested in environments with less anthropogenic activities.