中国南海红树内生真菌No.1403次级代谢物的研究

中国南海红树的内生真菌No 140 3是由香港城市大学Vrijmoed教授等分离出来的 .其发酵液粗提物有明显的抗菌活性 ,对酵母菌和霉菌均有抑制作用 .采用硅胶柱层析、核磁共振、质谱 ,红外光谱和X光结晶衍射等方法对该菌静置培养的发酵液化学成分进行研究 ,发现真菌No 140 3能产生灰黄霉素A和多种醌类抗生素B ,C ,D及其它化合物 ,其中C未见文献报道 .A的产量很高 ,约为 2 0mg/L ,本研究表明No 140 3是有应用价值的灰黄霉素新的产生菌 .

A New Cytotoxic Sterol Produced by an Endophytic Fungus from Castaniopsis fissa at the South China Sea Coast

A new sterol, ergosta-8(9),22-diene-3,5,6,7-tetraol(3β,5α,6β,7α,22E)(A) together with three known sterols: 3β, 5α,6β-trihydroxyergosta-7,22-diene (B), 3β-hydroxy-5α,8α- epidioxyer-gosta-6,22-diene (C) and ergosterol (D) were isolated from the mycelia of an unidentified endophytic fungus separated from Castaniopsis fissa (chestnut tree). Compound A exhibited potent selective cytotoxicity against Bel-7402, NCI4460 and L-02 cell lines with IC50 values 8.445, 5.03, 13.621 μg/mL, respectively.

Profile of Bionectriaceae,Calcarisporiaceae,Hypocreaceae,Nectriaceae,Tilachlidiaceae,Ijuhyaceae fam.nov.,Stromatonectriaceae fam.nov.and Xanthonectriaceae fam.nov

This paper provides outlines for Bionectriaceae,Calcarisporiaceae,Hypocreaceae,Nectriaceae,Tilachlidiaceae,Ijuhy-aceae,Stromatonectriaceae and Xanthonectriaceae with taxonomic treatments.We provide up-to-date DNA sequence-based phylogenies including combined gene analysis of ITS,LSU,rpb2,tef1 and tub2 for Hypocreales and accept 17 families.Three new families and 12 new species are introduced with descriptions and illustrations,while 13 new records and one new species combination are provided.Here we mainly detail the taxonomy of Bionectriaceae,Hypocreaceae,Nectriaceae and Tilachlidiaceae,Ijuhyaceae fam.nov.,Stromatonectriaceae fam.nov.and Xanthonectriaceae fam.nov.are introduced in this study based on phenotypic and molecular analyses.For each family we provide a list of accepted genera,the taxo-nomic history,morphological descriptions,taxonomic placement based on DNA sequence data and illustrate the type genus.Representatives of each family are illustrated based on the type herbarium material or fresh specimens where available,or provide relevant references.Notes on ecological and economic importance of the families are also given.

Taxonomic monograph of epifoliar fungi

Epifoliar fungi are one of the significant fungal groups typically living on the surface of leaves. They are usually recordedas saprobes, obligate parasites and commensals and are widely distributed in tropical and subtropical regions. Numerousgenera within this group remain inadequately understood, primarily attributed to limited taxonomic knowledge and insufficientmolecular data. Furthermore, the taxonomic delineation of epifoliar fungi remained uncertain, with scattered andliterature-based data often intermixed with other follicolous fungi. Herein, a comprehensive taxonomic monograph of 124genera in (32) Asterinales, (18) Capnodiales, (15) Chaetothyriales, (8) Meliolales, (8) Micropeltidales, (10) Microthyriales,(32) Parmulariales and (1) Zeloasperisporiales was provided re-describing with illustrations and line drawings. Notes onecological and economic importance of the families are also provided. Representatives type herbarium materials of Campoapulcherrima, Cycloschizon brachylaenae, Ferrarisia philippina, Hysterostomella guaranitica, Palawaniella orbiculata andPseudolembosia orbicularis of Parmulariaceae were re-examined and provided updated illustrations with descriptions. Abackbone phylogenetic tree and divergence estimation analysis for epifoliar fungi based on LSU and 5.8s ITS sequence dataare provided.

DISCOMYCETES: the apothecial representatives of the phylum Ascomycota

Discomycetes are an artificial grouping of apothecia-producing fungi in the phylum Ascomycota.Molecular-based studies have revealed that the discomycetes can be found among ten classes of Ascomycota.The classification of discomycetes has been a major challenge due to the lack of a clear understanding of the important morphological characters,as well as a lack of reference strains.In this review,we provide a historical perspective of discomycetes,notes on their morphology(including both asexual and sexual morphs),ecology and importance,an outline of discomycete families and a synoptical cladogram of currently accepted families in Ascomycota showing their systematic position.We also calculated evolutionary divergence times for major discomycetous taxa based on phylogenetic relationships using a combined LSU,SSU and RPB2 data set from 175 strains and fossil data.Our results confirm that discomycetes are found in two major subphyla of the Ascomycota:Taphrinomycotina and Pezizomycotina.The taxonomic placement of major discomycete taxa is briefly discussed.The most basal group of discomycetes is the class Neolectomycetes,which diverged from other Taphrinomycotina around 417 MYA(216–572),and the most derived group of discomycetes,the class Lecanoromycetes,diverged from Eurotiomycetes around 340 MYA(282–414).Further clarifications based on type specimens,designation of epitypes or reference specimens from fresh collections,and multi-gene analyses are needed to determine the taxonomic arrangement of many discomycetes.

Occurrence and geographical distribution of mangrove fungi

This is a multidimensional review of mangrove fungi occurring as saprobes,pathogens and endophytes of a wide range of host substrates and those isolated from the water columns and sediments in mangroves.Eight-hundred and fifty taxa including 658 that are supported by both morphology and molecular data and 192 with only morphological data are listed.These constitute Ascomycota,the dominant group with 773 species,and 58 Basidiomycota,one Blastocladiomycota,five Chytridiomycota,and 13 Mucoromycota.This study also includes data on mangrove yeasts 103 Ascomycota,39 Basidi-omycota and 193 taxa isolated from sediments.Endophytes isolated from submerged parts of mangrove plants total 38.The most specious orders of mangrove fungi are Pleosporales 133,Saccharomycetales 102,Microascales 101,Eurotiales 87,Hypocreales 60 and Xylariales 54.Speciose genera include Candida 39,Aspergillus 53,Penicillium 17 and Corollospora 16.The highest number of mangrove fungi have been recorded from the Pacific Ocean 553,which is the largest ocean,followed by Indian 408 and Atlantic Oceans 259.Geographical distribution of mangrove fungi varied from ocean to ocean with only 109 taxa common to the Atlantic,Indian and Pacific Oceans.Of the various countries reported for mangrove fungi,India accommodates the highest number(339)followed by Thailand 303,Malaysia 171,Florida Everglades,USA 134 and Brunei 134.A total of 60 different mangrove plants and their associates have been surveyed for mangrove fungi.These results are discussed and compared with previous studies.