Anti-inflammatory and antioxidant potential capacities of AD-MSCs and BM-MSCs in suppressing pancreaticβ-cells auto-immunity and apoptosis in rats with T1DM induced model

Since Type 1 diabetes(T1DM)occurs whenβ-cells mass is reduced to less than 20%of the normal level due to autoimmune destruction of cells resulting in the inability to secrete insulin,preservation or replenishment of the functionalβ-cells mass has become a major therapeutic focus for this diabetic type treatment.Thus,this 4-week work plan was designed to determine which mesenchymal stem cells(MSCs)type is more appropriate to alleviate pancreatic hazards resulting from diabetes induction;via tracking a comparative study between MSCs derived from adipose tissue(AD-MSCs)and from bone marrow(BM-MSCs)in management of T1DM considering their immunomodulatory,anti-apoptotic and antioxidative roles.Rats were divided randomly into 4 groups;control,STZdiabetic(D),D+AD-MSCs,and D+BM-MSCs groups.Both stem cells types in this study were allogenic.Herein,both oxidative stress and antioxidant markers were evaluated using colorimetric analysis,while inflammatory,immune and apoptotic markers were assessed through flow cytometric analysis.Results showed that diabetic rats treated with either AD-MSCs or BM-MSCs exhibited marked pancreatic antioxidant and anti-inflammatory activities that were able to initiate pancreatic immunomodulation and reducingβ-cells apoptotic death,thus,help to restore their normal insulin secretion and hypoglycemic abilities.However,AD-MSCs injection was shown to be superior as a pancreatic regenerative tool in overcoming diabetes;owing to their marked antioxidant,anti-inflammatory,immunomodulatory,and anti-apoptotic characteristics over BM-MSCs treatment.

The potency of N,N′-diphenyl-1,4-phenylenediamine and adiposederived stem cell co-administration in alleviating hepatorenal dysfunction complications associated with type 1 diabetes mellitus in rats

Background:The increasing occurrence of diabetes mellitus(DM)noted worldwide has considerably elicited concern in the recent past.DM is associated with elevated vascular complications,morbidity,mortality,and poor quality of life.In this context,mesenchymal stem cells(MSCs)have shown significant therapeutic potentialities in managing and curing type 1 DM owing to their self-renewable,immunosuppressive,and differentiation capacities.We investigated the potential action of N,N′-diphenyl-1,4-phenylenediamine(DPPD),a well-known synthetic antioxidant to enhance the therapeutic ability of the adipose-derived stem cells(AD-MSCs)in alleviating kidney and liver complications in diabetic rats.Methods:Over the four weeks of experiments,albino male rats(n=36)were split into six test groups:control,DPPD(250 mg/kg,i.p.),STZ-diabetic(D),D+DPPD,D+AD-MSCs(1×10^(6)cell/rat,i.v.),and D+AD-MSCs+DPPD treated groups.Results:Significant declines in the renal and hepatic oxidative stress markers(MDA,ROS,and AGEs)were observed coupled with a significant elevation in many antioxidant marker levels(GSH,SOD,CAT,GPx,HO-1,and TAC)in the diabetic rats treated with either DPPD or AD-MSCs or their co-administered injection compared to the diabetic untreated rats.This was suggested to be the leading cause of amelioration of the kidney functions(as measured by urea,uric acid,and creatine levels)and liver functions(as evidenced by the levels of AST,ALT,ALP,bilirubin,total proteins,albumin,and globulins).Conclusion:DPPD and AD-MSCs co-administration showed superior results in terms of the enhancement of the relative hepato-renal function,indicating the beneficial role of DPPD supplementation in increasing the therapeutic potential of AD-MSCs.

Investigation of the antioxidant defensive role of both AD-MSCs and BM-MSCs in modulating the alteration in the oxidative stress status in various STZ-diabetic rats’tissues

Diabetes mellitus(DM)could negatively affect patients’health via inducing a lot of serious functional hazards in many tissues’cells at molecular levels.Recently,many scientists had proposed stem cell therapy being an appropriate alternative treatment protocol for numerous health threatening issues including diabetes.Therefore,the current study was designed to investigate the antioxidant potentiality of two MSCs types in alleviating tissues’oxidative stress dramatic elevation resulting as a consequence of Type 1 DM induction.In our 4 weeks study,animals were divided into four groups:control group,STZ-diabetic group(D),D+AD-MSCs group and D+BM-MSCs group.Data reported that diabetic rats treated with either AD-MSCs or BM-MSCs exhibited a marvelous body tissues(Pancreas,Liver and Kidney)enhancing capabilities in attenuating the oxidative stress status;as evidenced by XO,ROS,and MDA levels down-regulation;with a general concomitant elevation in the antioxidants’content;evidenced by many enzymatic and non-enzymatic antioxidants up-regulation;relative to the diabetic untreated group.Interestingly,comparing both treatments with each other and to control group,most of the measured parameters were reverted back to near normal levels after AD-MSCs injection;which clearly point out their stunning health benefits and superiority as anti-diabetic agent in overcoming different tissues’complications;owing to their marked cytoprotective and regenerative potentialities.