Meningeal lymphatic vessels(mLVs)play a pivotal role in regulating metabolic waste from cerebrospinal fluid(CSF).However,the current limitations in field of view and resolution of existing imaging techniques impede un...Meningeal lymphatic vessels(mLVs)play a pivotal role in regulating metabolic waste from cerebrospinal fluid(CSF).However,the current limitations in field of view and resolution of existing imaging techniques impede understanding the stereoscopic morphology and dynamic behavior of mLVs in vivo.Here,we utilized dual-contrast functional photoacoustic microscopy to achieve wide-field intravital imaging of the lymphatic system,including mLVs and glymphatic pathways.The stereoscopic photoacoustic microscopy based on opto-acoustic confocal features has a depth imaging capability of 3.75 mm,facilitating differentiation between mLVs on the meninges and glymphatic pathways within the brain parenchyma.Subsequently,using this imaging technique,we were able to visualize the dynamic drainage of mLVs and identify a peak drainage period occurring around 20–40 min after injection,along with determining the flow direction from CSF to lymph nodes.Inspiringly,in the Alzheimer’s disease(AD)mouse model,we observed that AD mice exhibit a~70%reduction in drainage volume of mLVs compared to wild-type mice.With the development of AD,there is be continued decline in mLVs drainage volume.This finding clearly demonstrates that the AD mouse model has impaired CSF drainage.Our study opens up a horizon for understanding the brain’s drainage mechanism and dissecting mLVs-associated neurological disorders.展开更多
Photoacoustic endomicroscopy combined with ultrasound(PAEM-US)has been a long-standing expectation for gastrointestinal tumor examination.Here,we introduce a prototype disposable PAEM-US catheter and corresponding pow...Photoacoustic endomicroscopy combined with ultrasound(PAEM-US)has been a long-standing expectation for gastrointestinal tumor examination.Here,we introduce a prototype disposable PAEM-US catheter and corresponding power interface unit,featuring catheter switchability,self-internal three-dimensional scanning,and system repeatability for gastrointestinal endoscopy.By utilizing high-fluence relays,cascade insertion loss of the optical waveguide is minimized to 0.6 dB with a high performance of power resistance,and a focus-customizable acousto-optic coaxial probe is designed for high-sensitivity optical-resolution photoacoustic imaging.Imaging capability was demonstrated with in vivo anatomical imaging at 30 frames per second.Imaging results showed co-registered microscopic visualization of the microvascular and stratification of the rat colorectum with lateral resolution of 18μm and axial resolution of 63μm,holding great potential in the clinical detection of gastrointestinal diseases.展开更多
基金the National Natural Science Foundation of China(6233500762305118)+4 种基金the Natural Science Foundation of Guangdong Province(2022A1515010548)the Science and Technology Program of Guangzhou(2019050001)the Key Research and Development Plan of Guangzhou(202206010094)the STI2030-Major Projects(2022ZD0212200)the China Postdoctoral Science Foundation(2022M721223).
文摘Meningeal lymphatic vessels(mLVs)play a pivotal role in regulating metabolic waste from cerebrospinal fluid(CSF).However,the current limitations in field of view and resolution of existing imaging techniques impede understanding the stereoscopic morphology and dynamic behavior of mLVs in vivo.Here,we utilized dual-contrast functional photoacoustic microscopy to achieve wide-field intravital imaging of the lymphatic system,including mLVs and glymphatic pathways.The stereoscopic photoacoustic microscopy based on opto-acoustic confocal features has a depth imaging capability of 3.75 mm,facilitating differentiation between mLVs on the meninges and glymphatic pathways within the brain parenchyma.Subsequently,using this imaging technique,we were able to visualize the dynamic drainage of mLVs and identify a peak drainage period occurring around 20–40 min after injection,along with determining the flow direction from CSF to lymph nodes.Inspiringly,in the Alzheimer’s disease(AD)mouse model,we observed that AD mice exhibit a~70%reduction in drainage volume of mLVs compared to wild-type mice.With the development of AD,there is be continued decline in mLVs drainage volume.This finding clearly demonstrates that the AD mouse model has impaired CSF drainage.Our study opens up a horizon for understanding the brain’s drainage mechanism and dissecting mLVs-associated neurological disorders.
基金National Natural Science Foundation of China(62005084,61627827,6182250)Natural Science Foundation of Guangdong Province(2022A1515010548,2022A1515011247,2019A1515011399)+1 种基金Science and Technology Program of Guangzhou(202206010094,2019050001)Special Fund of Guangdong Academy of Sciences(2021GDASYL-20210103031)。
文摘Photoacoustic endomicroscopy combined with ultrasound(PAEM-US)has been a long-standing expectation for gastrointestinal tumor examination.Here,we introduce a prototype disposable PAEM-US catheter and corresponding power interface unit,featuring catheter switchability,self-internal three-dimensional scanning,and system repeatability for gastrointestinal endoscopy.By utilizing high-fluence relays,cascade insertion loss of the optical waveguide is minimized to 0.6 dB with a high performance of power resistance,and a focus-customizable acousto-optic coaxial probe is designed for high-sensitivity optical-resolution photoacoustic imaging.Imaging capability was demonstrated with in vivo anatomical imaging at 30 frames per second.Imaging results showed co-registered microscopic visualization of the microvascular and stratification of the rat colorectum with lateral resolution of 18μm and axial resolution of 63μm,holding great potential in the clinical detection of gastrointestinal diseases.
