This report describes in vitro micropropagation of Boscia senegalensis,so-called famine foods,that helped the people in Darfur and Kordofan,Sudan survive during the 1984-1985 famine.Four types of explants prepared fro...This report describes in vitro micropropagation of Boscia senegalensis,so-called famine foods,that helped the people in Darfur and Kordofan,Sudan survive during the 1984-1985 famine.Four types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1-5 mg/L 6-benzyladenine (BA).The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA,while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L BA.The shoot cluster,when subcultured to its same medium,significantly increased the rate of shoot multiplication by the end of the third subculture.The maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium.In vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete plantlets.B.senegalensis-regenerated plantlets obtained in vitro for the first time,were hardened and 95% survived under greenhouse conditions.展开更多
文摘This report describes in vitro micropropagation of Boscia senegalensis,so-called famine foods,that helped the people in Darfur and Kordofan,Sudan survive during the 1984-1985 famine.Four types of explants prepared from green mature zygotic embryos were cultured on Murashige and Skoog (MS) medium augmented with 1-5 mg/L 6-benzyladenine (BA).The highest number of shoots per explant (14.3±0.9) was achieved on MS medium supplemented with 3 mg/L BA,while the highest shoot length [(3.5±0.4) cm] was obtained with 1 mg/L BA.The shoot cluster,when subcultured to its same medium,significantly increased the rate of shoot multiplication by the end of the third subculture.The maximum mean number of shoots per explant (86.5±3.6) was produced after three multiplication cycles on 3 mg/L BA-supplemented medium.In vitro induced shoots were excised and rooted on half strength MS medium fortified with 0.25 mg/L indole-3-butyric acid (IBA) to obtain complete plantlets.B.senegalensis-regenerated plantlets obtained in vitro for the first time,were hardened and 95% survived under greenhouse conditions.
