Objective: Colon cancer is one of the most common human malignancies. Cancer stem cells(CSCs), despite being only a small subset of cancer cells, have the capability to self-renew and sustain the tumor. They also have...Objective: Colon cancer is one of the most common human malignancies. Cancer stem cells(CSCs), despite being only a small subset of cancer cells, have the capability to self-renew and sustain the tumor. They also have the ability to proliferate. Multiple CSCs-associated markers have been identified in colon cancer including CD133, ALDH1 and β-catenin. The aim of the work was to study the prognostic value of CSCs markers(CD133, ALDH1 and β-catenin), as well as their relationship to clinicopathological features of colon cancer. Methods: CD133, ALDH1 and β-catenin proteins expression was assessed immunohistochemically in a series of colon cancers and their prognostic significance was evaluated. Results: CD133 expression showed significant relationship to tumor stage and lymph node metastasis(P-value 0.004 & < 0.001 respectively), and near significant relationship to liver metastasis(P-value 0.092). ALDH1 was significantly associated with tumor grade, stage and nodal metastasis(P-value 0.021, 0.001 and 0.026 respectively), but its relationship to liver metastasis was near significant(P-value 0.068). Nuclear β-catenin was significantly related to tumor grade, stage, nodal and liver metastasis(P-value 0.001, < 0.001, < 0.001 and 0.008 respectively). Overall survival(OS) was associated inversely with CD133, ALDH1 positivity, and directly with nuclear β-catenin positivity(P-value < 0.001, 0.0001 and < 0.001 respectively). Also recurrence free survival(RFS) was associated inversely with CD133, ALDH1 and directly with nuclear β-catenin positivity(P-value 0.0001, 0.001 and < 0.001 respectively). Conclusion: CD133, ALDH1 and β-catenin expressions of tumor cells have significant impact upon malignant progression of colon cancer and thus patient survival and tumor recurrence. Hence they can be used to predict outcome of colon cancer patients.展开更多
Objective:Previous reports have shown that the gene promoter region of retinoic acid receptor β(RARβ) was hypermethylated in cervical carcinoma,implying the inhibition of gene transcription.The aim of this study was...Objective:Previous reports have shown that the gene promoter region of retinoic acid receptor β(RARβ) was hypermethylated in cervical carcinoma,implying the inhibition of gene transcription.The aim of this study was to investigate the association of cervical cancer development with the RARβ gene expression at the mRNA and protein level to assess the impact of RARβ as a marker for early detection of the cancer.Methods:We collected 126 cases of formalin fixed and paraffin embedded cervical tissue specimens as well as 37 cases of fresh tissue samples from women with cervicitis,cervical intraepithelial neoplasia(CIN) and cervical squamous cell carcinoma(CSCC).The RARβ mRNA and protein expression was detected by quantitative RT-PCR and immunohistochemistry,respectively.Results:(1) The mRNA expression of RARβ in CIN and cervical cancer was markedly decreased compared to cervicitis with a statistically very significant difference,but no difference was found between CIN and cervical cancer.(2) RARβ protein was normally expressed in the epithelial cells of cervicitis and partially lost in a few cases,but with the development of cervical lesion pathogenesis and cancer,a significant loss of protein expression was detected in CIN(38%) and CSCC(57%) compared to cervicitis(P < 0.01).Conclusion:The downregulation of RARβ transcription or loss of protein expression is an important indicator of cervical cancer and its precursur lesions.The detection of RARβ expression coupled with aberrant methylation of the gene may become a biomarker for the early prognosis or diagnosis of the cancer.展开更多
文摘Objective: Colon cancer is one of the most common human malignancies. Cancer stem cells(CSCs), despite being only a small subset of cancer cells, have the capability to self-renew and sustain the tumor. They also have the ability to proliferate. Multiple CSCs-associated markers have been identified in colon cancer including CD133, ALDH1 and β-catenin. The aim of the work was to study the prognostic value of CSCs markers(CD133, ALDH1 and β-catenin), as well as their relationship to clinicopathological features of colon cancer. Methods: CD133, ALDH1 and β-catenin proteins expression was assessed immunohistochemically in a series of colon cancers and their prognostic significance was evaluated. Results: CD133 expression showed significant relationship to tumor stage and lymph node metastasis(P-value 0.004 & < 0.001 respectively), and near significant relationship to liver metastasis(P-value 0.092). ALDH1 was significantly associated with tumor grade, stage and nodal metastasis(P-value 0.021, 0.001 and 0.026 respectively), but its relationship to liver metastasis was near significant(P-value 0.068). Nuclear β-catenin was significantly related to tumor grade, stage, nodal and liver metastasis(P-value 0.001, < 0.001, < 0.001 and 0.008 respectively). Overall survival(OS) was associated inversely with CD133, ALDH1 positivity, and directly with nuclear β-catenin positivity(P-value < 0.001, 0.0001 and < 0.001 respectively). Also recurrence free survival(RFS) was associated inversely with CD133, ALDH1 and directly with nuclear β-catenin positivity(P-value 0.0001, 0.001 and < 0.001 respectively). Conclusion: CD133, ALDH1 and β-catenin expressions of tumor cells have significant impact upon malignant progression of colon cancer and thus patient survival and tumor recurrence. Hence they can be used to predict outcome of colon cancer patients.
文摘Objective:Previous reports have shown that the gene promoter region of retinoic acid receptor β(RARβ) was hypermethylated in cervical carcinoma,implying the inhibition of gene transcription.The aim of this study was to investigate the association of cervical cancer development with the RARβ gene expression at the mRNA and protein level to assess the impact of RARβ as a marker for early detection of the cancer.Methods:We collected 126 cases of formalin fixed and paraffin embedded cervical tissue specimens as well as 37 cases of fresh tissue samples from women with cervicitis,cervical intraepithelial neoplasia(CIN) and cervical squamous cell carcinoma(CSCC).The RARβ mRNA and protein expression was detected by quantitative RT-PCR and immunohistochemistry,respectively.Results:(1) The mRNA expression of RARβ in CIN and cervical cancer was markedly decreased compared to cervicitis with a statistically very significant difference,but no difference was found between CIN and cervical cancer.(2) RARβ protein was normally expressed in the epithelial cells of cervicitis and partially lost in a few cases,but with the development of cervical lesion pathogenesis and cancer,a significant loss of protein expression was detected in CIN(38%) and CSCC(57%) compared to cervicitis(P < 0.01).Conclusion:The downregulation of RARβ transcription or loss of protein expression is an important indicator of cervical cancer and its precursur lesions.The detection of RARβ expression coupled with aberrant methylation of the gene may become a biomarker for the early prognosis or diagnosis of the cancer.
