Marine microorganisms were considered to be important sources of marine bioactive compounds.The major objective of the study was to isolate and characterize bacteria with antimicrobial activities from the various mari...Marine microorganisms were considered to be important sources of marine bioactive compounds.The major objective of the study was to isolate and characterize bacteria with antimicrobial activities from the various marine environment of Egypt.In this respect,thirty-five bacterial isolates were recovered from sediment samples collected from different spots along the Egyptian Red Sea coastline and Alexandria coastline during the summer season of 2017 and 2018.According to the morphological,physiological,and biochemical characteristics,the bacterial isolates were clustered into 13 groups designated as A,B,…,M.And,14 Gram-negative and 21 Grampositive bacteria were determined.The isolated bacterial strains were screened for their potentiality for antimicrobial agent(s)production against ten indicator strains.Strain Mo13 was showed high antimicrobial activity against all empirical strains.Subsequently,the most promising marine bacterial isolate with code MO13 was identified as Bacillus zhangzhouensis OMER4 according to the phenotypic characterization through morphological,physiological,and biochemical tests as well as genotypic characterization through the 16 S rDNA technique.The bioactive components were extracted with ethyl acetate,then analyzed using GC-MS and the substantial component was recognized as phenol,2,4-bis(1,1-dimethyl ethyl).展开更多
文摘Marine microorganisms were considered to be important sources of marine bioactive compounds.The major objective of the study was to isolate and characterize bacteria with antimicrobial activities from the various marine environment of Egypt.In this respect,thirty-five bacterial isolates were recovered from sediment samples collected from different spots along the Egyptian Red Sea coastline and Alexandria coastline during the summer season of 2017 and 2018.According to the morphological,physiological,and biochemical characteristics,the bacterial isolates were clustered into 13 groups designated as A,B,…,M.And,14 Gram-negative and 21 Grampositive bacteria were determined.The isolated bacterial strains were screened for their potentiality for antimicrobial agent(s)production against ten indicator strains.Strain Mo13 was showed high antimicrobial activity against all empirical strains.Subsequently,the most promising marine bacterial isolate with code MO13 was identified as Bacillus zhangzhouensis OMER4 according to the phenotypic characterization through morphological,physiological,and biochemical tests as well as genotypic characterization through the 16 S rDNA technique.The bioactive components were extracted with ethyl acetate,then analyzed using GC-MS and the substantial component was recognized as phenol,2,4-bis(1,1-dimethyl ethyl).
