The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus us...The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus using all known callus inducible media have been unsatisfactory. Here we developed and described an efficient protocol for callus induction from B73 maize seedlings. The protocol is based on well known callus inducible media CM4C where we have sequentially subtracted some chemical compounds and added some new compounds mediating cell proliferations. This newly described protocol was able to induce callus production in a wide range of crop species including rice and soybean. We found that cell proliferation factors, NAA (auxin analog) and 2,4 D (auxin influx carrier) were not only very crucial but required for positive B73 maize callus induction. The absence of one or the other will lead to the failure of B73 maize callus production. The well known CM4C callus induction composition lacks NAA. Our findings will advance genetic studies of maize mutants generated from B73 genotype background.展开更多
Our study aimed at assessing the effects of 3 Plants Growth Promoting Rhizobacteria (PGPR) either singly or in combination on maize growth under laboratory and greenhouse conditions. Seeds were inoculated with single ...Our study aimed at assessing the effects of 3 Plants Growth Promoting Rhizobacteria (PGPR) either singly or in combination on maize growth under laboratory and greenhouse conditions. Seeds were inoculated with single and combined solution of 108 CFU/ml of Rhizobacteria. Seeds were not inoculated for the control variant. The highest germination percentage was obtained with the combination of Pseudomonas fluorescens and Pseudomonas putida. This combination also recorded the best vigor index, plants circumferences number of leaves and the leaf area. The maximal heights of plants were observed with seeds treated with Azospirillum lipoferum with an increase of 37.32%. The highest rates of underground dry matter were recorded with A. lipoferum, with an increase of more than 56% comparative to control, while the combination P. fluorescens and P. putida increased the aerial dry matter of 59.11%. Finally, the highest value of the aerial biomass was obtained with the plants treated with the combination of P. fluorescens and P. putida and the highest underground biomass was obtained with plants treated only with A. lipoferum. These results suggest that specific combinations of PGPR can be considered as efficient alternative biofertilizers to promote maize seed germination, biomass and crop yield.展开更多
文摘The wild type maize genotype, B73, is not amenable for callus production and an efficient protocol for B73 maize callus induction has never been reported up-to-date. Scientific efforts in producing B73 maize callus using all known callus inducible media have been unsatisfactory. Here we developed and described an efficient protocol for callus induction from B73 maize seedlings. The protocol is based on well known callus inducible media CM4C where we have sequentially subtracted some chemical compounds and added some new compounds mediating cell proliferations. This newly described protocol was able to induce callus production in a wide range of crop species including rice and soybean. We found that cell proliferation factors, NAA (auxin analog) and 2,4 D (auxin influx carrier) were not only very crucial but required for positive B73 maize callus induction. The absence of one or the other will lead to the failure of B73 maize callus production. The well known CM4C callus induction composition lacks NAA. Our findings will advance genetic studies of maize mutants generated from B73 genotype background.
基金thank the International Foundation of Science(IFS)which granted NOUMAVO Pacôme(Research grant No.C/5252-1).
文摘Our study aimed at assessing the effects of 3 Plants Growth Promoting Rhizobacteria (PGPR) either singly or in combination on maize growth under laboratory and greenhouse conditions. Seeds were inoculated with single and combined solution of 108 CFU/ml of Rhizobacteria. Seeds were not inoculated for the control variant. The highest germination percentage was obtained with the combination of Pseudomonas fluorescens and Pseudomonas putida. This combination also recorded the best vigor index, plants circumferences number of leaves and the leaf area. The maximal heights of plants were observed with seeds treated with Azospirillum lipoferum with an increase of 37.32%. The highest rates of underground dry matter were recorded with A. lipoferum, with an increase of more than 56% comparative to control, while the combination P. fluorescens and P. putida increased the aerial dry matter of 59.11%. Finally, the highest value of the aerial biomass was obtained with the plants treated with the combination of P. fluorescens and P. putida and the highest underground biomass was obtained with plants treated only with A. lipoferum. These results suggest that specific combinations of PGPR can be considered as efficient alternative biofertilizers to promote maize seed germination, biomass and crop yield.
