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The LEAFY floral regulator displays pioneer transcription factor properties 被引量:1
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作者 Xuelei Lai Romain Blanc-Mathieu +14 位作者 Loïc GrandVuillemin Ying Huang Arnaud Stigliani Jeremy Lucas emmanuel thevenon Jeanne Loue-Manifel Laura Turchi Hussein Daher Eugenia Brun-Hernandez Gilles Vachon David Latrasse Moussa Benhamed Renaud Dumas Chloe Zubieta François Parcy 《Molecular Plant》 SCIE CAS CSCD 2021年第5期829-837,共9页
Pioneer transcription factors(TFs)are a special category of TFs with the capacity to bind to closed chromatin regions in which DNA is wrapped around histones and may be highly methylated.Subsequently,pioneer TFs are a... Pioneer transcription factors(TFs)are a special category of TFs with the capacity to bind to closed chromatin regions in which DNA is wrapped around histones and may be highly methylated.Subsequently,pioneer TFs are able to modify the chromatin state to initiate gene expression.In plants,LEAFY(LFY)is a master floral regulator and has been suggested to act as a pioneer TF in Arabidopsis.Here,we demonstrate that LFY is able to bind both methylated and non-methylated DNA using a combination of in vitro genomewide binding experiments and structural modeling.Comparisons between regions bound by LFY in vivo and chromatin accessibility data suggest that a subset of LFY bound regions is occupied by nucleosomes.We confirm that LFY is able to bind nucleosomal DNA in vitro using reconstituted nucleosomes.Finally,we show that constitutive LFY expression in seedling tissues is sufficient to induce chromatin accessibility in the LFY direct target genes APETALA1 and AGAMOUS.Taken together,our study suggests that LFY possesses key pioneer TF features that contribute to launching the floral gene expression program. 展开更多
关键词 flower development pioneer transcription factor gene regulation chromatin remodeling
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