Objective:To explore the cytotoxicity effects of Caspian cobra(Naja oxiana or N.oxiana) venom on hepatocytes and mitochondria obtained from the liver of HCC rats.Methods:In this study,HCC was induced by diethylnitrosa...Objective:To explore the cytotoxicity effects of Caspian cobra(Naja oxiana or N.oxiana) venom on hepatocytes and mitochondria obtained from the liver of HCC rats.Methods:In this study,HCC was induced by diethylnitrosamine(DEN),as an initiator,and 2-acetylaminofluorene(2-AAF),as a promoter.Rat liver hepatocytes and mitochondria for evaluation of the selective cytotoxic effect of N.oxiana venom were isolated and mitochondria and cellular parameters related to apoptosis signaling were then determined.Results:Our results showed a raise in mitochondrial reactive oxygen species(ROS)level,swelling in mitochondria,mitochondrial membrane potential(Djm) collapse and release of cytochrome c after exposure of mitochondria only isolated from the HCC group with the crude venom of the N.oxiana(12.5,25,and 50 mg/m L).This crude venom also induced caspase-3 activation(P < 0.001) in the hepatocytes obtained only from the HCC rat liver.Conclusions:Based on the over all results,we suggested that N.oxiana may be considered as a promising complementary therapeutic agent for the treatment of HCC.展开更多
基金supported by Shahid Beheshti University of Medical Sciences,Deputy of Research(Grant No.1394-1-95-11835,2015)
文摘Objective:To explore the cytotoxicity effects of Caspian cobra(Naja oxiana or N.oxiana) venom on hepatocytes and mitochondria obtained from the liver of HCC rats.Methods:In this study,HCC was induced by diethylnitrosamine(DEN),as an initiator,and 2-acetylaminofluorene(2-AAF),as a promoter.Rat liver hepatocytes and mitochondria for evaluation of the selective cytotoxic effect of N.oxiana venom were isolated and mitochondria and cellular parameters related to apoptosis signaling were then determined.Results:Our results showed a raise in mitochondrial reactive oxygen species(ROS)level,swelling in mitochondria,mitochondrial membrane potential(Djm) collapse and release of cytochrome c after exposure of mitochondria only isolated from the HCC group with the crude venom of the N.oxiana(12.5,25,and 50 mg/m L).This crude venom also induced caspase-3 activation(P < 0.001) in the hepatocytes obtained only from the HCC rat liver.Conclusions:Based on the over all results,we suggested that N.oxiana may be considered as a promising complementary therapeutic agent for the treatment of HCC.
