In vitro Regeneration of First-generation Inbred Progenies of Cucurbita pepo L. Double Haploids

Using seeds of inbred progenies of Cucurbita pepo L. DH12-11409 double haplolds as experimental materials, the effects of different hormone combinations, explant types and seedling ages on adventitious bud regeneration and rooting of C. pepo L. were investigated. According to the results, inoculating cotyledonary nodes of yellow-green cotyledons from 5-d-old C. pepo L. double haploids to MS ＋ 30 g/L Suc ＋ 8 g/L Agar ＋ 2.0 mg/L 6-BA ＋ 0.1 mg/L NAA led to the best results with the induction frequency of 90.0% and differentiation coefficient of 8.5. MS medium with addition of 0.05 or 0.1 mg/L NAA led to the highest rooting rate. Regenerated seedlings with 5 - 6 true leaves exhibited the highest survival rate of 90.0%, which was the optimal period for domestication and transplanting of regenerated seedlings. This study laid a solid foundation for high-efficiency utilization of heterosis of C. pepo L.

Tribological study of two ammonium chloride-decanoic acid deep eutectic solvents(DESs)as high-performance lubricants

Deep eutectic solvents(DESs)are acknowledged as a novel class of functional liquid.DESs share similar physical properties with ionic liquids(ILs)and have the potential to be a novel class of lubricants.In this study,two DESs,namely tetrabutylammonium chloride-decanoic acid DES(C4-DES)and methyl tricaprylmethylammonium chloride-decanoic acid DES(C8-DES),were synthesized,and their physico-chemical properties and tribological performances were evaluated.Post-analysis of the rubbing surfaces used multiple techniques to gain insights into the lubrication mechanisms.Results show that the coefficient of friction(COF)and wear were reduced by approximately 29%and 91%for the C4-DES,and 36%and 94%for the C8-DES,compared to an ester base oil.The friction reduction behavior of the DESs is attributed to the monolayer adsorption of the polar group in the decanoic acid(DEAC),whose effectiveness is affected by the component of the ammonium salts in the DESs and the operating temperatures.In addition to the adsorbed film,worn surface analysis revealed that an ultra-thin tribochemical film with a thickness of 3–7 nm was formed on the surfaces lubricated with the C8-DES.The composition of the film was studied,and the lubrication mechanisms of the two DESs were discussed.

Hippocampal sclerosis in women with temporal lobe epilepsy:seizure and pregnancy outcomes

Background Temporal lobe epilepsy with hippocampal sclerosis(TLE-HS)is typically resistant to pharmacological interventions;however,achieving seizure freedom is possible through surgery.Our objective was to focus on the pregnancy and seizure outcomes during pregnancy of women with TLE-HS,and aim to identify predictors of seizure control.Methods The West China Registry of Pregnancy of Women with Epilepsy(WCPR_EPi)was a monocentric prospective cohort study of women with epilepsy(WWE).We screened women with TLE-HS in this database.Their clinical profile,anti-seizure medication(ASM)use,and pregnancy outcomes were extracted from the records of the registry(2010–2023).Results Out of 2320 WWE followed up,47 pregnancies in women with TLE-HS were identified and analyzed.Seizure exacerbation occurred in 40.4%of pregnancies,and seizure freedom was present in 34.0%of these during pregnancy.Factors associated with seizure exacerbation during pregnancy was ASM non-adherence(odds ratio[OR]=7.00,95%confidence interval[CI]1.43–34.07,P=0.016).The surgery group showed a significantly higher seizure freedom rate(OR=6.87,95%CI 1.02–46.23,P=0.016)and lower rate of induced labor(0.0%vs 26.5%,P=0.047)compared to the medically-treated group alone.Caesarean section was chosen in 77.1%of cases due to seizure concerns,with comparable in epilepsy-related(n=20)and obstetric causes(n=24).No major congenital malformations were reported.Conclusions Surgical treatment before pregnancy appears to offer a higher chance of seizure freedom compared to medication alone.Most of women with TLE-HS can deliver healthy offspring regardless of suboptimal seizure control and unwarranted concerns.