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Widely Untargeted Metabolome Profiling Provides Insight into Browning and Nutritional Quality Changes in Short-Term Stored Fresh-Cut Potato(Solanum tuberosum L.)Shreds
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作者 Liping Hong enming he +3 位作者 Wenhui Zhang Mingqiong Zheng Jingwen Wu Wenhua Wang 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第10期2785-2805,共21页
Potato(Solanum tuberosum L.)is susceptible to enzymatic browning after fresh processing,resulting in color change and potential alteration in the nutritional quality.In this study,a popular potato cultivar,Feiwuruita,... Potato(Solanum tuberosum L.)is susceptible to enzymatic browning after fresh processing,resulting in color change and potential alteration in the nutritional quality.In this study,a popular potato cultivar,Feiwuruita,was used to profile the metabolites involved in color and nutritional quality changes in fresh shreds stored at 0 and 4 h at 25°C(designated CK and CK4H,respectively).The shreds turned brown within 4 h of storage.In all,723 metabolites consisting 12 classes of compounds were detected in the samples,largely lipids,phenolic acids,alkaloids,amino acids and derivatives,flavonoids,organic acids,nucleotides and derivatives.Of these,163 metabolites accumulated differentially between CK and CK4H shreds.Polyphenolic compounds(phenolic acids and flavonoids)mostly increased in the shreds after 4 h storage.Conversely,the short-term storage drastically reduced lipid compounds(25 LysoPC and 19 LysoPE),while essential alkaloids and terpenoid compounds that are beneficial to human health increased in accumulation.The findings present global metabolome and nutritional composition changes in short-term stored shreds of Feiwuruita.This study provides important foundation for future studies on browning prevention/reduction and for better utilization of Feiwuruita. 展开更多
关键词 Enzymatic browning colorimetric values METABOLITES phenylpropanoid biosynthesis polyphenol oxidases activity
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麻疯树精细胞分离 被引量:2
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作者 朱云 陈美灵 +1 位作者 何恩铭 田惠桥 《植物学研究》 2012年第2期36-40,共5页
将麻疯树成熟花粉置于一定渗透剂溶液中,可使其破裂,释放出其中的一对精细胞。释放的精细胞在20~30 min后用FDA染色仍可显示具有活性的荧光。用显微操作仪将释放的精细胞收集,得到一定数量具有活性的精细胞群体。研究了不同渗透剂浓度... 将麻疯树成熟花粉置于一定渗透剂溶液中,可使其破裂,释放出其中的一对精细胞。释放的精细胞在20~30 min后用FDA染色仍可显示具有活性的荧光。用显微操作仪将释放的精细胞收集,得到一定数量具有活性的精细胞群体。研究了不同渗透剂浓度下释放的精细胞状态,比较了不同渗透剂的精细胞释放率及保持精细胞活性的最适浓度,以7.5%的葡萄糖溶液分离麻疯树精细胞的最佳浓度。麻疯树精细胞的成功分离为其离体受精实验奠定了一定基础,也为研究麻疯树精细胞发育的分子生物学实验提供了材料。 展开更多
关键词 麻疯树 精细胞 分离
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